RT Journal Article SR Electronic(1) A1 Yamada, Kentaro A1 Noguchi, Kazuko A1 Nonaka, Daichi A1 Morita, Muneshin A1 Yasuda, Aiko A1 Kawazato, Hiroaki A1 Nishizono, AkiraYR 2013 T1 Addition of a single N-glycan to street rabies virus glycoprotein enhances virus production JF Journal of General Virology, VO 94 IS 2 SP 270 OP 275 DO https://doi.org/10.1099/vir.0.047852-0 PB Microbiology Society, SN 1465-2099, AB Most street rabies virus G proteins have two N-glycosylation sites, i.e. Asn37 and Asn319, whereas additional sites are found in fixed (laboratory adapted) viruses. In this study, we performed a pseudotyped virus assay using G-deficient rabies virus and demonstrated that single-N-glycan additions to the G protein of street rabies virus strain 1088, which are found in adapted strains, enhanced virus production in neural and non-neural cell lines, while additions to Asn194 or Asn247 enhanced production greatly. Moreover, we found that N-glycan additions at Asn194 or Asn247 facilitated the production of cell-associated virus. In contrast, deletion of the sequon at Asn37 reduced viral production, while a deletion at Asn319 resulted in extensive loss of production. Furthermore, G proteins lacking an N-glycan at Asn319 failed to fold into their correct structure and lost their fusion activity, indicating that Asn319 N-glycosylation is important for the functional expression of street virus G proteins., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.047852-0