@article{mbs:/content/journal/jgv/10.1099/vir.0.047571-0, author = "Loughran, G. and Libbey, J. E. and Uddowla, S. and Scallan, M. F. and Ryan, M. D. and Fujinami, R. S. and Rieder, E. and Atkins, J. F.", title = "Theiler’s murine encephalomyelitis virus contrasts with encephalomyocarditis and foot-and-mouth disease viruses in its functional utilization of the StopGo non-standard translation mechanism", journal= "Journal of General Virology", year = "2013", volume = "94", number = "2", pages = "348-353", doi = "https://doi.org/10.1099/vir.0.047571-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.047571-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The picornaviruses’ genome consists of a positive-sense ssRNA. Like many picornaviruses, cardioviruses synthesize two distinct polyprotein precursors from adjacent but non-overlapping genome segments. Both the [L-1ABCD-2A] and the [2BC-3ABCD] polyproteins are proteolytically processed to yield mature capsid and non-structural proteins, respectively. An unusual translational event, known as ‘StopGo’ or ‘Stop-Carry on’, is responsible for the release of the [L-1ABCD-2A] polyprotein from the ribosome and synthesis of the N-terminal amino acid of the [2BC-3ABCD] polyprotein. A common feature of these viruses is the presence of a highly conserved signature sequence for StopGo: –D(V/I)ExNPG↓P–, where –D(V/I)ExNPG are the last 7 aa of 2A, and the last P- is the first amino acid of 2B. Here, we report that, in contrast to encephalomyocarditis virus and foot-and-mouth disease virus, a functional StopGo does not appear to be essential for Theiler’s murine encephalomyelitis virus viability when tested in vitro and in vivo.", }