1887

Abstract

Although equine infectious anemia (EIA) was described more than 150 years ago, complete genomic sequences have only been obtained from two field strains of EIA virus (EIAV), EIAV and EIAV. In 2011, EIA was detected within the distinctive feral Misaki horse population that inhabits the Toi-Cape area of southern Japan. Complete proviral sequences comprising a novel field strain were amplified directly from peripheral blood of one of these EIAV-infected horses and characterized by nucleotide sequencing. The complete provirus of Miyazaki2011-A strain is 8208 bp in length with an overall genomic organization typical of EIAV. However, this field isolate possesses just 77.2 and 78.7 % nucleotide sequence identity with the EIAV and EIAV strains, respectively, while similarity plot analysis suggested all three strains arose independently. Furthermore, phylogenetic studies using sequences obtained from all EIAV-infected Misaki horses against known viral strains strongly suggests these Japanese isolates comprise a separate monophyletic group.

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2013-02-01
2019-09-23
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References

  1. Cappelli K. , Capomaccio S. , Cook F. R. , Felicetti M. , Marenzoni M. L. , Coppola G. , Verini-Supplizi A. , Coletti M. , Passamonti F. . ( 2011; ). Molecular detection, epidemiology, and genetic characterization of novel European field isolates of equine infectious anemia virus. . J Clin Microbiol 49:, 27–33. [CrossRef] [PubMed]
    [Google Scholar]
  2. Cheevers W. P. , McGuire T. C. . ( 1985; ). Equine infectious anemia virus: immunopathogenesis and persistence. . Rev Infect Dis 7:, 83–88. [CrossRef] [PubMed]
    [Google Scholar]
  3. Clabough D. L. , Gebhard D. , Flaherty M. T. , Whetter L. E. , Perry S. T. , Coggins L. , Fuller F. J. . ( 1991; ). Immune-mediated thrombocytopenia in horses infected with equine infectious anemia virus. . J Virol 65:, 6242–6251.[PubMed]
    [Google Scholar]
  4. Coggins L. , Norcross N. L. , Nusbaum S. R. . ( 1972; ). Diagnosis of equine infectious anemia by immunodiffusion test. . Am J Vet Res 33:, 11–18.[PubMed]
    [Google Scholar]
  5. Cook R. F. , Cook S. J. , Bolin P. S. , Howe L. J. , Zhou W. , Montelaro R. C. , Issel C. J. . ( 2005; ). Genetic immunization with codon-optimized equine infectious anemia virus (EIAV) surface unit (SU) envelope protein gene sequences stimulates immune responses in ponies. . Vet Microbiol 108:, 23–37. [CrossRef] [PubMed]
    [Google Scholar]
  6. Craigo J. K. , Leroux C. , Howe L. , Steckbeck J. D. , Cook S. J. , Issel C. J. , Montelaro R. C. . ( 2002; ). Transient immune suppression of inapparent carriers infected with a principal neutralizing domain-deficient equine infectious anaemia virus induces neutralizing antibodies and lowers steady-state virus replication. . J Gen Virol 83:, 1353–1359.[PubMed]
    [Google Scholar]
  7. Craigo J. K. , Barnes S. , Zhang B. , Cook S. J. , Howe L. , Issel C. J. , Montelaro R. C. . ( 2009; ). An EIAV field isolate reveals much higher levels of subtype variability than currently reported for the equine lentivirus family. . Retrovirology 6:, 95. [CrossRef] [PubMed]
    [Google Scholar]
  8. Dong J. B. , Zhu W. , Cook F. R. , Goto Y. , Horii Y. , Haga T. . ( 2012; ). Development of a nested PCR assay to detect equine infectious anemia proviral DNA from peripheral blood of naturally infected horses. . Arch Virol 10:, 10–12.[PubMed]
    [Google Scholar]
  9. Hammond S. A. , Li F. , McKeon B. M. Sr , Cook S. J. , Issel C. J. , Montelaro R. C. . ( 2000; ). Immune responses and viral replication in long-term inapparent carrier ponies inoculated with equine infectious anemia virus. . J Virol 74:, 5968–5981. [CrossRef] [PubMed]
    [Google Scholar]
  10. Harrold S. M. , Cook S. J. , Cook R. F. , Rushlow K. E. , Issel C. J. , Montelaro R. C. . ( 2000; ). Tissue sites of persistent infection and active replication of equine infectious anemia virus during acute disease and asymptomatic infection in experimentally infected equids. . J Virol 74:, 3112–3121. [CrossRef] [PubMed]
    [Google Scholar]
  11. Issel C. J. , Coggins L. . ( 1979; ). Equine infectious anemia: current knowledge. . J Am Vet Med Assoc 174:, 727–733.[PubMed]
    [Google Scholar]
  12. Kobayashi K. , Kono Y. . ( 1967; ). Serial passages of equine infectious anemia virus in horse leukocyte culture. . Natl Inst Anim Health Q (Tokyo) 7:, 1–7.[PubMed]
    [Google Scholar]
  13. Kono Y. , Kobayashi K. , Fukunaga Y. . ( 1970; ). Immunization of horses against equine infectious anemia (EIA) with an attenuated EIA virus. . Natl Inst Anim Health Q (Tokyo) 10:, 113–122.[PubMed]
    [Google Scholar]
  14. Kono Y. , Kobayashi K. , Fukunaga Y. . ( 1971; ). Serological comparison among various strains of equine infectious anemia virus. . Arch Gesamte Virusforsch 34:, 202–208. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kono Y. , Hirasawa K. , Fukunaga Y. , Taniguchi T. . ( 1976; ). Recrudescence of equine infectious anemia by treatment with immunosuppressive drugs. . Natl Inst Anim Health Q (Tokyo) 16:, 8–15.[PubMed]
    [Google Scholar]
  16. Leroux C. , Cadoré J. L. , Montelaro R. C. . ( 2004; ). Equine infectious anemia virus (EIAV): what has HIV’s country cousin got to tell us?. Vet Res 35:, 485–512. [CrossRef] [PubMed]
    [Google Scholar]
  17. Lole K. S. , Bollinger R. C. , Paranjape R. S. , Gadkari D. , Kulkarni S. S. , Novak N. G. , Ingersoll R. , Sheppard H. W. , Ray S. C. . ( 1999; ). Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. . J Virol 73:, 152–160.[PubMed]
    [Google Scholar]
  18. MacLachlan N. J. , Dubovi E. J. . ( 2011; ). Equine Infectious Anemia Virus (Retroviridae). . In Fenners Veterinary Virology, , 4th edn., pp. 269–270. London:: Elsevier;.
    [Google Scholar]
  19. Nagarajan M. M. , Simard C. . ( 2007; ). Gag genetic heterogeneity of equine infectious anemia virus (EIAV) in naturally infected horses in Canada. . Virus Res 129:, 228–235. [CrossRef] [PubMed]
    [Google Scholar]
  20. Petropoulos C. J. . ( 1997; ). Retroviral taxonomy, protein structures, sequences, and genetic maps. . In Retroviruses, pp. 757. Edited by Coffin J. M. . . New York:: Cold Spring Harbor Laboratory Press;.
    [Google Scholar]
  21. Sa Filho D. J. , Sanabani S. , Diaz R. S. , Munerato P. , Brunstein A. , Fusuma E. , Sabino E. C. , Janini L. M. . ( 2005; ). Analysis of full-length human immunodeficiency virus type 1 genome reveals a variable spectrum of subtypes B and f recombinants in São Paulo, Brazil. . AIDS Res Hum Retroviruses 21:, 145–151. [CrossRef] [PubMed]
    [Google Scholar]
  22. Sellon D. C. , Fuller F. J. , McGuire T. C. . ( 1994; ). The immunopathogenesis of equine infectious anemia virus. . Virus Res 32:, 111–138. [CrossRef] [PubMed]
    [Google Scholar]
  23. Sherefa K. , Johansson B. , Salminen M. , Sönnerborg A. . ( 1998; ). Full-length sequence of human immunodeficiency virus type 1 subtype A, recombined with subtype C in the env V3 domain. . AIDS Res Hum Retroviruses 14:, 289–292. [CrossRef] [PubMed]
    [Google Scholar]
  24. Su L. , Graf M. , Zhang Y. , von Briesen H. , Xing H. , Köstler J. , Melzl H. , Wolf H. , Shao Y. , Wagner R. . ( 2000; ). Characterization of a virtually full-length human immunodeficiency virus type 1 genome of a prevalent intersubtype (C/B’) recombinant strain in China. . J Virol 74:, 11367–11376. [CrossRef] [PubMed]
    [Google Scholar]
  25. Tabuchi E. , Tanaka K. , Akiyama Y. , Katada M. , Yamamoto H. , Sakaki K. , Yoshino T. . ( 1965; ). Long-term reservation of equine infectious anemia virus in a horse. . Jap Vet Med Ass 18:, 437–442.[CrossRef]
    [Google Scholar]
  26. Tabuchi E. , Katada M. , Hirasawa M. , Fujino M. . ( 1967; ). An outbreak of equine infectious anemia in race horses - epizootical, clinical, and hematological observations. . Bull Nat Inst Anim Hlth 55:, 45–51.
    [Google Scholar]
  27. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  28. Tu Y. B. , Zhou T. , Yuan X. F. , Qiu H. J. , Xue F. , Sun C. Q. , Wang L. , Wu D. L. , Peng J. M. . & other authors ( 2007; ). Long terminal repeats are not the sole determinants of virulence for equine infectious anemia virus. . Arch Virol 152:, 209–218. [CrossRef] [PubMed]
    [Google Scholar]
  29. Zheng Y. H. , Sentsui H. , Kono Y. , Ikuta K. . ( 2000; ). Mutations occurring during serial passage of Japanese equine infectious anemia virus in primary horse macrophages. . Virus Res 68:, 93–98. [CrossRef] [PubMed]
    [Google Scholar]
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