Skip to content
1887

Journal of General Virology header logo Journal of General Virology

Volume 93, Issue 12

Incorporation of GP64 into nucleopolyhedrovirus enhances virus infectivity and No Access

Abstract

The envelope fusion proteins of baculoviruses, glycoprotein GP64 from group I nucleopolyhedrovirus (NPV) or the F protein from group II NPV and granulovirus, are essential for baculovirus morphogenesis and infectivity. The F protein is considered the ancestral baculovirus envelope fusion protein, while GP64 is a more recent evolutionary introduction into baculoviruses and exhibits higher fusogenic activity than the F protein. Each of the fusion proteins is required by the respective virus to spread infection within larval tissues. A recombinant NPV (HearNPV) expressing GP64 from multiple nucleopolyhedrovirus, vHaBac, was constructed, which still retained the native F protein, and its infectivity was assayed and . Analyses by one-step growth curve to determine viral titre and by quantitative PCR to determine viral DNA copy number showed that vHaBac was more infectious than the control, vHaBac. The gene () was reintroduced into the recombinant viruses and bioassays showed that vHaBac accelerated the mortality of infected larvae compared with the vHaBac control, and the LC (median lethal concentration) of vHaBac- was reduced to approximately 20 % of that of vHaBac. Therefore, incorporation of GP64 into HearNPV budded virions improved virus infectivity both and . The construction of this bivalent virus with a more efficient fusion protein could improve the use of baculoviruses in different areas such as gene therapy and biocontrol.

  • Received:
  • Accepted:
  • Published Online:
© 2012 SGM
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.046458-0
2012-12-01
2025-05-22
Loading full text...

Full text loading...

References

  1. Blissard G. W. 1996; Baculovirus–insect cell interactions. Cytotechnology 20:73–93 [View Article][PubMed]
     [Google Scholar]
  2. Blissard G. W., Rohrmann G. F. 1989; Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 170:537–555 [View Article][PubMed]
     [Google Scholar]
  3. Blissard G. W., Wenz J. R. 1992; Baculovirus gp64 envelope glycoprotein is sufficient to mediate pH-dependent membrane fusion. J Virol 66:6829–6835[PubMed]
     [Google Scholar]
  4. Boyce F. M., Bucher N. L. 1996; Baculovirus-mediated gene transfer into mammalian cells. Proc Natl Acad Sci U S A 93:2348–2352 [View Article][PubMed]
     [Google Scholar]
  5. Hayakawa T., Rohrmann G. F., Hashimoto Y. 2000; Patterns of genome organization and content in lepidopteran baculoviruses. Virology 278:1–12 [View Article][PubMed]
     [Google Scholar]
  6. Hefferon K. L., Oomens A. G., Monsma S. A., Finnerty C. M., Blissard G. W. 1999; Host cell receptor binding by baculovirus GP64 and kinetics of virion entry. Virology 258:455–468 [View Article][PubMed]
     [Google Scholar]
  7. Herniou E. A., Luque T., Chen X., Vlak J. M., Winstanley D., Cory J. S., O’Reilly D. R. 2001; Use of whole genome sequence data to infer baculovirus phylogeny. J Virol 75:8117–8126 [View Article][PubMed]
     [Google Scholar]
  8. IJkel W. F. J., Westenberg M., Goldbach R. W., Blissard G. W., Vlak J. M., Zuidema D. 2000; A novel baculovirus envelope fusion protein with a proprotein convertase cleavage site. Virology 275:30–41 [View Article][PubMed]
     [Google Scholar]
  9. Kost T. A., Condreay J. P. 2002; Recombinant baculoviruses as mammalian cell gene-delivery vectors. Trends Biotechnol 20:173–180 [View Article][PubMed]
     [Google Scholar]
  10. Liang C., Song J., Chen X. 2005; The GP64 protein of Autographa californica multiple nucleopolyhedrovirus rescues Helicoverpa armigera nucleopolyhedrovirus transduction in mammalian cells. J Gen Virol 86:1629–1635 [View Article][PubMed]
     [Google Scholar]
  11. Long G., Westenberg M., Wang H., Vlak J. M., Hu Z. 2006; Function, oligomerization and N-linked glycosylation of the Helicoverpa armigera single nucleopolyhedrovirus envelope fusion protein. J Gen Virol 87:839–846 [View Article][PubMed]
     [Google Scholar]
  12. Lung O. Y., Cruz-Alvarez M., Blissard G. W. 2003; Ac23, an envelope fusion protein homolog in the baculovirus Autographa californica multicapsid nucleopolyhedrovirus, is a viral pathogenicity factor. J Virol 77:328–339 [View Article][PubMed]
     [Google Scholar]
  13. Oomens A. G., Blissard G. W. 1999; Requirement for GP64 to drive efficient budding of Autographa californica multicapsid nucleopolyhedrovirus. Virology 254:297–314 [View Article][PubMed]
     [Google Scholar]
  14. Pearson M. N., Rohrmann G. F. 2002; Transfer, incorporation, and substitution of envelope fusion proteins among members of the Baculoviridae, Orthomyxoviridae, and Metaviridae (insect retrovirus) families. J Virol 76:5301–5304 [View Article][PubMed]
     [Google Scholar]
  15. Pearson M. N., Groten C., Rohrmann G. F. 2000; Identification of the Lymantria dispar nucleopolyhedrovirus envelope fusion protein provides evidence for a phylogenetic division of the Baculoviridae. J Virol 74:6126–6131 [View Article][PubMed]
     [Google Scholar]
  16. Pearson M. N., Russell R. L., Rohrmann G. F. 2001; Characterization of a baculovirus-encoded protein that is associated with infected-cell membranes and budded virions. Virology 291:22–31 [View Article][PubMed]
     [Google Scholar]
  17. Song J., Wang R., Deng F., Wang H., Hu Z. 2008; Functional studies of per os infectivity factors of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus. J Gen Virol 89:2331–2338 [View Article][PubMed]
     [Google Scholar]
  18. Summers M. D. 1971; Electron microscopic observations on granulosis virus entry, uncoating and replication processes during infection of the midgut cells of Trichoplusia ni. J Ultrastruct Res 35:606–625 [View Article][PubMed]
     [Google Scholar]
  19. Sun X., Zhang G., Zhang Z., Hu Z.-H., Vlak J. M., Arif B. M. 1998; In vivo cloning of Helicoverpa armigera single nucleocapsid nuclear polyhedrosis virus genotypes. Virol Sin 13:83–88
     [Google Scholar]
  20. Tani H., Nishijima M., Ushijima H., Miyamura T., Matsuura Y. 2001; Characterization of cell-surface determinants important for baculovirus infection. Virology 279:343–353 [View Article][PubMed]
     [Google Scholar]
  21. Volkman L. E., Goldsmith P. A. 1985; Mechanism of neutralization of budded Autographa californica nuclear polyhedrosis virus by a monoclonal antibody: inhibition of entry by adsorptive endocytosis. Virology 143:185–195 [View Article][PubMed]
     [Google Scholar]
  22. Wang H., Deng F., Pijlman G. P., Chen X., Sun X., Vlak J. M., Hu Z. 2003; Cloning of biologically active genomes from a Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus isolate by using a bacterial artificial chromosome. Virus Res 97:57–63 [View Article][PubMed]
     [Google Scholar]
  23. Wang S., Wang H., Deng F., Wu D., Long G., Yuan L., Hu Z. 2005; Construction of a recombinant HaSNPV expressing GP64. Virol Sin 20:79–83
     [Google Scholar]
  24. Wang M., Tan Y., Yin F., Deng F., Vlak J. M., Hu Z., Wang H. 2008a; The F-like protein Ac23 enhances the infectivity of the budded virus of gp64-null Autographa californica multinucleocapsid nucleopolyhedrovirus pseudotyped with baculovirus envelope fusion protein F. J Virol 82:9800–9804 [View Article][PubMed]
     [Google Scholar]
  25. Wang M., Tan Y., Yin F., Deng F., Vlak J. M., Hu Z., Wang H. 2008b; The F protein of Helicoverpa armigera single nucleopolyhedrovirus can be substituted functionally with its homologue from Spodoptera exigua multiple nucleopolyhedrovirus. J Gen Virol 89:791–798 [View Article][PubMed]
     [Google Scholar]
  26. Wang M., Yin F., Shen S., Tan Y., Deng F., Vlak J. M., Hu Z., Wang H. 2010; Partial functional rescue of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus infectivity by replacement of F protein with GP64 from Autographa californica multicapsid nucleopolyhedrovirus. J Virol 84:11505–11514 [View Article][PubMed]
     [Google Scholar]
  27. Westenberg M., Vlak J. M. 2008; GP64 of group I nucleopolyhedroviruses cannot readily rescue infectivity of group II f-null nucleopolyhedroviruses. J Gen Virol 89:424–431 [View Article][PubMed]
     [Google Scholar]
  28. Whitford M., Stewart S., Kuzio J., Faulkner P. 1989; Identification and sequence analysis of a gene encoding gp67, an abundant envelope glycoprotein of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol 63:1393–1399[PubMed]
     [Google Scholar]
/content/journal/jgv/10.1099/vir.0.046458-0
Loading
Incorporation of GP64 into Helicoverpa armigera nucleopolyhedrovirus enhances virus infectivity in vivo and in vitro
J. Gen. Virol. 93, 2705 (2012); https://doi.org/10.1099/vir.0.046458-0
/content/journal/jgv/10.1099/vir.0.046458-0
/content/journal/jgv/10.1099/vir.0.046458-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error