1887

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Volume 93, Issue 12

Incorporation of GP64 into nucleopolyhedrovirus enhances virus infectivity and Free

Abstract

The envelope fusion proteins of baculoviruses, glycoprotein GP64 from group I nucleopolyhedrovirus (NPV) or the F protein from group II NPV and granulovirus, are essential for baculovirus morphogenesis and infectivity. The F protein is considered the ancestral baculovirus envelope fusion protein, while GP64 is a more recent evolutionary introduction into baculoviruses and exhibits higher fusogenic activity than the F protein. Each of the fusion proteins is required by the respective virus to spread infection within larval tissues. A recombinant NPV (HearNPV) expressing GP64 from multiple nucleopolyhedrovirus, vHaBac, was constructed, which still retained the native F protein, and its infectivity was assayed and . Analyses by one-step growth curve to determine viral titre and by quantitative PCR to determine viral DNA copy number showed that vHaBac was more infectious than the control, vHaBac. The gene () was reintroduced into the recombinant viruses and bioassays showed that vHaBac accelerated the mortality of infected larvae compared with the vHaBac control, and the LC (median lethal concentration) of vHaBac- was reduced to approximately 20 % of that of vHaBac. Therefore, incorporation of GP64 into HearNPV budded virions improved virus infectivity both and . The construction of this bivalent virus with a more efficient fusion protein could improve the use of baculoviruses in different areas such as gene therapy and biocontrol.

  • Received:
  • Accepted:
  • Published Online:
© 2012 SGM
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2012-12-01
2023-03-20
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Incorporation of GP64 into Helicoverpa armigera nucleopolyhedrovirus enhances virus infectivity in vivo and in vitro
J. Gen. Virol. 93, 2705 (2012); https://doi.org/10.1099/vir.0.046458-0
/content/journal/jgv/10.1099/vir.0.046458-0
/content/journal/jgv/10.1099/vir.0.046458-0
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