1887

Abstract

Barley stripe mosaic virus North Dakota 18 (ND18), Beijing (BJ), Xinjiang (XJ), Type (TY) and CV21 strains are unable to infect the Bd3-1 inbred line, which harbours a resistance gene designated , but the Norwich (NW) strain is virulent on Bd3-1. Analysis of ND18 and NW genomic RNA reassortants and RNAβ mutants demonstrates that two amino acids within the helicase motif of the triple gene block 1 (TGB1) movement protein have major effects on their Bd3-1 phenotypes. Resistance to ND18 correlates with an arginine residue at TGB1 position 390 (R) and a threonine at position 392 (T), whereas the virulent NW strain contains lysines (K) at both positions. ND18 TGB1 R390K (TGB1) and TGB1 single substitutions, and an TGB1 double mutation resulted in systemic infections of Bd3-1. Reciprocal TGB1 substitutions into TGB1 (TGB1 and TGB1) failed to affect virulence, implying that K and K compensate for each other. In contrast, an TGB1 double mutant exhibited limited vascular movement in Bd3-1, but developed prominent necrotic streaks that spread from secondary leaf veins. This phenotype, combined with the appearance of necrotic spots in certain ND18 mutants, and necrosis and rapid wilting of Bd3-1 plants after BJ strain (TGB1) inoculations, show that Bd3-1 resistance is elicited by the TGB1 protein and suggest that it involves a hypersensitive response.

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2012-12-01
2020-01-27
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