1887

Abstract

and have co-existed in Italian tomato crops since 2002 and have reached equilibrium, with plants hosting molecules of both species plus their recombinants being the most frequent case. Recombination events are studied in field samples, as well as in experimental co-infections, when recombinants were detected as early as 45 days following inoculation. In both conditions, recombination breakpoints were essentially absent in regions corresponding to ORFs V2, CP and C4, whereas density was highest in the 3′-terminal portion of ORF C3, next to the region where the two transcription units co-terminate. The vast majority of breakpoints were mapped at antisense ORFs, supporting speculation that the rolling-circle replication mechanism, and the existence of sense and antisense ORFs on the circular genome, may result in clashes between replication and transcription complexes.

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2012-12-01
2019-09-16
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References

  1. Accotto G. P. , Bragaloni M. , Luison D. , Davino S. , Davino M. . ( 2003; ). First report of Tomato yellow leaf curl virus (TYLCV) in Italy. . Plant Pathol 52:, 799. [CrossRef]
    [Google Scholar]
  2. Baird H. A. , Galetto R. , Gao Y. , Simon-Loriere E. , Abreha M. , Archer J. , Fan J. , Robertson D. L. , Arts E. J. , Negroni M. . ( 2006; ). Sequence determinants of breakpoint location during HIV-1 intersubtype recombination. . Nucleic Acids Res 34:, 5203–5216. [CrossRef] [PubMed]
    [Google Scholar]
  3. Davino S. , Napoli C. , Davino M. , Accotto G. P. . ( 2006; ). Spread of Tomato yellow leaf curl virus in Sicily: partial displacement of another geminivirus originally present. . Eur J Plant Pathol 114:, 293–299. [CrossRef]
    [Google Scholar]
  4. Davino S. , Davino M. , Accotto G. P. . ( 2008; ). A single-tube PCR assay for detecting viruses and their recombinants that cause tomato yellow leaf curl disease in the Mediterranean basin. . J Virol Methods 147:, 93–98. [CrossRef] [PubMed]
    [Google Scholar]
  5. Davino S. , Napoli C. , Dellacroce C. , Miozzi L. , Noris E. , Davino M. , Accotto G. P. . ( 2009; ). Two new natural begomovirus recombinants associated with the tomato yellow leaf curl disease co-exist with parental viruses in tomato epidemics in Italy. . Virus Res 143:, 15–23. [CrossRef] [PubMed]
    [Google Scholar]
  6. Fauquet C. M. , Sawyer S. , Idris A. M. , Brown J. K. . ( 2005; ). Sequence analysis and classification of apparent recombinant begomoviruses infecting tomato in the Nile and Mediterranean basins. . Phytopathology 95:, 549–555. [CrossRef] [PubMed]
    [Google Scholar]
  7. García-Andrés S. , Monci F. , Navas-Castillo J. , Moriones E. . ( 2006; ). Begomovirus genetic diversity in the native plant reservoir Solanum nigrum: evidence for the presence of a new virus species of recombinant nature. . Virology 350:, 433–442. [CrossRef] [PubMed]
    [Google Scholar]
  8. García-Andrés S. , Accotto G. P. , Navas-Castillo J. , Moriones E. . ( 2007a; ). Founder effect, plant host, and recombination shape the emergent population of begomoviruses that cause the tomato yellow leaf curl disease in the Mediterranean basin. . Virology 359:, 302–312. [CrossRef] [PubMed]
    [Google Scholar]
  9. García-Andrés S. , Tomás D. M. , Sánchez-Campos S. , Navas-Castillo J. , Moriones E. . ( 2007b; ). Frequent occurrence of recombinants in mixed infections of tomato yellow leaf curl disease-associated begomoviruses. . Virology 365:, 210–219. [CrossRef] [PubMed]
    [Google Scholar]
  10. García-Arenal F. , Fraile A. , Malpica J. M. . ( 2001; ). Variability and genetic structure of plant virus populations. . Annu Rev Phytopathol 39:, 157–186.[PubMed] [CrossRef]
    [Google Scholar]
  11. Huson D. H. , Bryant D. . ( 2006; ). Application of phylogenetic networks in evolutionary studies. . Mol Biol Evol 23:, 254–267. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kheyr-Pour A. , Bendahmane M. , Matzeit V. , Accotto G. P. , Crespi S. , Gronenborn B. . ( 1991; ). Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted monopartite geminivirus. . Nucleic Acids Res 19:, 6763–6769. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lefeuvre P. , Lett J.-M. , Varsani A. , Martin D. P. . ( 2009; ). Widely conserved recombination patterns among single-stranded DNA viruses. . J Virol 83:, 2697–2707. [CrossRef] [PubMed]
    [Google Scholar]
  14. Martin D. P. , Biagini P. , Lefeuvre P. , Golden M. , Roumagnac P. , Varsani A. . ( 2011a; ). Recombination in eukaryotic single stranded DNA viruses. . Viruses 3:, 1699–1738. [CrossRef] [PubMed]
    [Google Scholar]
  15. Martin D. P. , Lefeuvre P. , Varsani A. , Hoareau M. , Semegni J.-Y. , Dijoux B. , Vincent C. , Reynaud B. , Lett J.-M. . ( 2011b; ). Complex recombination patterns arising during geminivirus coinfection preserve and demarcate biologically important intra-genome interaction networks. . PLoS Pathog 7:, 1–14. [CrossRef]
    [Google Scholar]
  16. Monci F. , Sánchez-Campos S. , Navas-Castillo J. , Moriones E. . ( 2002; ). A natural recombinant between the geminiviruses Tomato yellow leaf curl Sardinia virus and Tomato yellow leaf curl virus exhibits a novel pathogenic phenotype and is becoming prevalent in Spanish populations. . Virology 303:, 317–326. [CrossRef] [PubMed]
    [Google Scholar]
  17. Morilla G. , Krenz B. , Jeske H. , Bejarano E. R. , Wege C. . ( 2004; ). Tête à tête of Tomato yellow leaf curl virus and Tomato yellow leaf curl Sardinia virus in single nuclei. . J Virol 78:, 10715–10723. [CrossRef] [PubMed]
    [Google Scholar]
  18. Nagy P. D. . ( 2008; ). Recombination in plant RNA viruses. . In Plant Virus Evolution, pp. 133–156. Edited by Roossinck M. J. . . Berlin, Heidelberg:: Springer-Verlag;. [CrossRef]
    [Google Scholar]
  19. Noris E. , Hidalgo E. , Accotto G. P. , Moriones E. . ( 1994; ). High similarity among the tomato yellow leaf curl virus isolates from the west Mediterranean basin: the nucleotide sequence of an infectious clone from Spain. . Arch Virol 135:, 165–170. [CrossRef] [PubMed]
    [Google Scholar]
  20. Padidam M. , Sawyer S. , Fauquet C. M. . ( 1999; ). Possible emergence of new geminiviruses by frequent recombination. . Virology 265:, 218–225. [CrossRef] [PubMed]
    [Google Scholar]
  21. Sánchez-Campos S. , Díaz J. A. , Monci F. , Bejarano E. R. , Reina J. , Navas-Castillo J. , Aranda M. A. , Moriones E. . ( 2002; ). High genetic stability of the begomovirus Tomato yellow leaf curl Sardinia virus in southern Spain over an 8-year period. . Phytopathology 92:, 842–849. [CrossRef] [PubMed]
    [Google Scholar]
  22. Zhou X. P. , Liu Y. L. , Calvert L. , Munoz C. , Otim-Nape G. W. , Robinson D. J. , Harrison B. D. . ( 1997; ). Evidence that DNA-A of a geminivirus associated with severe cassava mosaic disease in Uganda has arisen by interspecific recombination. . J Gen Virol 78:, 2101–2111.[PubMed]
    [Google Scholar]
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