1887

Abstract

We have performed a series of studies to investigate the role of CD4 T-cells in the immune response to foot-and-mouth disease virus (FMDV) post-vaccination. Virus neutralizing antibody titres (VNT) in cattle vaccinated with killed FMD commercial vaccine were significantly reduced and class switching delayed as a consequence of rigorous CD4 T-cell depletion. Further studies were performed to examine whether the magnitude of T-cell proliferative responses correlated with the antibody responses. FMD vaccination was found to induce T-cell proliferative responses, with CD4 T-cells responding specifically to the FMDV antigen. In addition, gamma interferon (IFN-γ) was detected in the supernatant of FMDV antigen-stimulated PBMC and purified CD4 T-cells from vaccinated cattle. Similarly, intracellular IFN-γ could be detected specifically in purified CD4 T-cells after restimulation. It was not possible to correlate proliferative responses or IFN-γ production of PBMC with VNT, probably as a consequence of the induction of T-independent and T-dependent antibody responses and antigen non-specific T-cell responses. However, our studies demonstrate the importance of stimulating CD4 T-cell responses for the induction of optimum antibody responses to FMD-killed vaccines.

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2013-01-01
2020-01-22
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References

  1. Becker Y.. ( 1994;). Need for cellular and humoral immune responses in bovines to ensure protection from foot-and-mouth disease virus (FMDV)–a point of view. . Virus Genes 8:, 199–214. [CrossRef][PubMed]
    [Google Scholar]
  2. Brown F.. ( 1995;). Antibody recognition and neutralization of foot-and-mouth disease virus. . Semin Virol 6:, 243–248. [CrossRef]
    [Google Scholar]
  3. Collen T.. ( 1994;). Foot-and-mouth disease (Aphthovirus): viral T cell epitopes. . In Cell-Mediated Immunity in Ruminants, pp. 173–212. Edited by Goddeevis I. M. B. M. L... Boca Raton:: CRC Press;.
    [Google Scholar]
  4. DiMarchi R., Brooke G., Gale C., Cracknell V., Doel T., Mowat N.. ( 1986;). Protection of cattle against foot-and-mouth disease by a synthetic peptide. . Science 232:, 639–641. [CrossRef][PubMed]
    [Google Scholar]
  5. Gerner W., Denyer M. S., Takamatsu H. H., Wileman T. E., Wiesmüller K. H., Pfaff E., Saalmüller A.. ( 2006;). Identification of novel foot-and-mouth disease virus specific T-cell epitopes in c/c and d/d haplotype miniature swine. . Virus Res 121:, 223–228. [CrossRef][PubMed]
    [Google Scholar]
  6. Gerner W., Carr B. V., Wiesmüller K. H., Pfaff E., Saalmüller A., Charleston B.. ( 2007;). Identification of a novel foot-and-mouth disease virus specific T-cell epitope with immunodominant characteristics in cattle with MHC serotype A31. . Vet Res 38:, 565–572. [CrossRef][PubMed]
    [Google Scholar]
  7. Glass E. J., Oliver R. A., Collen T., Doel T. R., Dimarchi R., Spooner R. L.. ( 1991;). MHC class II restricted recognition of FMDV peptides by bovine T cells. . Immunology 74:, 594–599.[PubMed]
    [Google Scholar]
  8. Guzman E., Taylor G., Charleston B., Skinner M. A., Ellis S. A.. ( 2008;). An MHC-restricted CD8+ T-cell response is induced in cattle by foot-and-mouth disease virus (FMDV) infection and also following vaccination with inactivated FMDV. . J Gen Virol 89:, 667–675. [CrossRef][PubMed]
    [Google Scholar]
  9. Guzman E., Taylor G., Charleston B., Ellis S. A.. ( 2010;). Induction of a cross-reactive CD8+ T cell response following foot-and-mouth disease virus vaccination. . J Virol 84:, 12375–12384. [CrossRef][PubMed]
    [Google Scholar]
  10. Haberthur K., Engelmann F., Park B., Barron A., Legasse A., Dewane J., Fischer M., Kerns A., Brown M., Messaoudi I.. ( 2011;). CD4 T cell immunity is critical for the control of simian varicella virus infection in a nonhuman primate model of VZV infection. . PLoS Pathog 7:, e1002367. [CrossRef][PubMed]
    [Google Scholar]
  11. Hope J. C., Kwong L. S., Entrican G., Wattegedera S., Vordermeier H. M., Sopp P., Howard C. J.. ( 2002;). Development of detection methods for ruminant interleukin (IL)-12. . J Immunol Methods 266:, 117–126. [CrossRef][PubMed]
    [Google Scholar]
  12. Juleff N., Windsor M., Reid E., Seago J., Zhang Z., Monaghan P., Morrison I. W., Charleston B.. ( 2008;). Foot-and-mouth disease virus persists in the light zone of germinal centres. . PLoS ONE 3:, e3434. [CrossRef][PubMed]
    [Google Scholar]
  13. Juleff N., Windsor M., Lefevre E. A., Gubbins S., Hamblin P., Reid E., McLaughlin K., Beverley P. C., Morrison I. W., Charleston B.. ( 2009;). Foot-and-mouth disease virus can induce a specific and rapid CD4+ T-cell-independent neutralizing and isotype class-switched antibody response in naïve cattle. . J Virol 83:, 3626–3636. [CrossRef][PubMed]
    [Google Scholar]
  14. McCullough K. C., De Simone F., Brocchi E., Capucci L., Crowther J. R., Kihm U.. ( 1992;). Protective immune response against foot-and-mouth disease. . J Virol 66:, 1835–1840.[PubMed]
    [Google Scholar]
  15. Naessens J., Scheerlinck J. P., De Buysscher E. V., Kennedy D., Sileghem M.. ( 1998;). Effective in vivo depletion of T cell subpopulations and loss of memory cells in cattle using mouse monoclonal antibodies. . Vet Immunol Immunopathol 64:, 219–234. [CrossRef][PubMed]
    [Google Scholar]
  16. Nutt S. L., Tarlinton D. M.. ( 2011;). Germinal center B and follicular helper T cells: siblings, cousins or just good friends?. Nat Immunol 12:, 472–477. [CrossRef][PubMed]
    [Google Scholar]
  17. Paton D. J., Sumption K. J., Charleston B.. ( 2009;). Options for control of foot-and-mouth disease: knowledge, capability and policy. . Philos Trans R Soc Lond B Biol Sci 364:, 2657–2667. [CrossRef][PubMed]
    [Google Scholar]
  18. Pulendran B., Ahmed R.. ( 2011;). Immunological mechanisms of vaccination. . Nat Immunol 12:, 509–517. [CrossRef][PubMed]
    [Google Scholar]
  19. Stockinger B., Bourgeois C., Kassiotis G.. ( 2006;). CD4+ memory T cells: functional differentiation and homeostasis. . Immunol Rev 211:, 39–48. [CrossRef][PubMed]
    [Google Scholar]
  20. Zhu J., Paul W. E.. ( 2008;). CD4 T cells: fates, functions, and faults. . Blood 112:, 1557–1569. [CrossRef][PubMed]
    [Google Scholar]
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