1887

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Volume 94, Issue 1

Cell-dependent production of polyhedra and virion occlusion of multiple nucleopolyhedrovirus mutants and Free

Abstract

Members of the family are insect-specific dsDNA viruses that have been used for biological control of insect pests in agriculture and forestry, as well as in research and pharmaceutical protein expression in insect cells and larvae. multiple nucleopolyhedrovirus (AcMNPV) is the type species of the family . During infection of AcMNPV in permissive cells, mutants are positively selected, leading to the formation of the few polyhedra (FP) phenotype with reduced yield of polyhedra and reduced virion occlusion efficiency, which leads to decreased oral infectivity for insects. Here we report that polyhedra of AcMNPV mutants produced from different insect cell lines and insects have differences in larval infectivity, and that these variations are due to different virion occlusion efficiencies in these cell lines and insects. Polyhedra of AcMNPV mutants produced from cells (21 and 9, derived from ) and larvae had poorer virion occlusion efficiency than those from Hi5 cells (derived from ) and larvae, based on immunoblots, DNA isolation and larval oral infection analysis. AcMNPV mutants formed clusters of FP and many polyhedra (MP) in the fat body cells of both and larvae. Transmission electron microscopy revealed that the nature of virion occlusion of AcMNPV mutants was dependent on the different cells of the fat body tissue. Taken together, these results indicate that the FP phenotype and virion occlusion efficiency of mutants are influenced by the host insect cells.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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2013-01-01
2024-04-25
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Cell-dependent production of polyhedra and virion occlusion of Autographa californica multiple nucleopolyhedrovirus fp25k mutants in vitro and in vivo
J. Gen. Virol. 94, 177 (2013); https://doi.org/10.1099/vir.0.045591-0
/content/journal/jgv/10.1099/vir.0.045591-0
/content/journal/jgv/10.1099/vir.0.045591-0
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