@article{mbs:/content/journal/jgv/10.1099/vir.0.045302-0, author = "Yan, Guolin and Zhang, Yusi and Ma, Ying and Yi, Jing and Liu, Bei and Xu, Zhuwei and Zhang, Yun and Zhang, Chunmei and Zhang, Fanglin and Xu, Zhikai and Yang, Angang and Zhuang, Ran and Jin, Boquan", title = "Identification of a novel B-cell epitope of Hantaan virus glycoprotein recognized by neutralizing 3D8 monoclonal antibody", journal= "Journal of General Virology", year = "2012", volume = "93", number = "12", pages = "2595-2600", doi = "https://doi.org/10.1099/vir.0.045302-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.045302-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Hantaan virus (HTNV), a member of the family Bunyaviridae, is a major agent causing haemorrhagic fever with renal syndrome, a high-mortality-rate disease threatening approximately 150 000 people around the world yearly. The 3D8 mAb displays a neutralizing activity to HTNV infection. In this study, the B-cell epitopes of HTNV glycoproteins (GPs) were finely mapped by peptide scanning. A new B-cell epitope 882GFLCPEFPGSFRKKC896 of HTNV, which locates on Gc, has been screened out from a set of 15-mer synthesized peptides covering the full-length of HTNV-GPs. It has been shown by the alanine-scanning technique that 885C, 893R, 894K, 895K and 896C are the key amino acids of the binding sites of the GPs. The implications of identifying a novel B-cell epitope for hantavirus immunology and vaccinology are discussed.", }