Characterization of naturally Epstein–Barr virus-infected gastric carcinoma cell line YCCEL1 Free

Abstract

Epstein–Barr virus (EBV) is a herpesvirus associated with lymphomas and carcinomas. While EBV-associated epithelial cell lines are good model systems to investigate the role of EBV in carcinoma, only a few cell lines are available as they are hard to acquire. A greater variety of naturally EBV-infected cell lines which are derived from tumour patients are needed to represent various features of EBVaGC. We characterized cell line YCCEL1, established from a Korean EBVaGC patient, to ascertain whether it can be used to study the roles of EBV in EBVaGC. The expression of EBV genes and cell surface markers was examined by hybridization, RT-PCR, Western blot analysis, immunofluorescence assay and Northern blot analysis. EBV episomal status was analysed by Southern blotting and real-time PCR. This cell line expressed EBV nuclear antigen 1 (EBNA1) and latent membrane protein 2A (LMP2A), but not EBNA2, LMP2B nor LMP1. The majority of the lytic proteins were not detected in YCCEL1 cells either before or after treatment with 12--tetradecanoylphorbol-13-acetate. YCCEL1 cells expressed BART microRNAs (miRNAs) at high level but did not express BHRF1 miRNAs. YCCEL1 cells expressed cytokeratin, but not CD21 and CD19, suggesting CD21-independent EBV infection. The latent EBV gene and EBV miRNA expression pattern of YCCEL1 cells closely resembled that of general EBVaGC cases. Our results support the value of YCCEL1 cells as a good model system to study the role of EBV in gastric carcinogenesis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.045237-0
2013-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/94/3/497.html?itemId=/content/journal/jgv/10.1099/vir.0.045237-0&mimeType=html&fmt=ahah

References

  1. Andersson M. 1975; Amounts of Epstein-Barr virus DNA in somatic cell hybrids between Burkitt lymphoma-derived cell lines. J Virol 16:1345–1347[PubMed]
    [Google Scholar]
  2. Bernasconi M., Berger C., Sigrist J. A., Bonanomi A., Sobek J., Niggli F. K., Nadal D. 2006; Quantitative profiling of housekeeping and Epstein-Barr virus gene transcription in Burkitt lymphoma cell lines using an oligonucleotide microarray. Virol J 3:43 [View Article][PubMed]
    [Google Scholar]
  3. Chesnokova L. S., Nishimura S. L., Hutt-Fletcher L. M. 2009; Fusion of epithelial cells by Epstein-Barr virus proteins is triggered by binding of viral glycoproteins gHgL to integrins αvβ6 or αvβ8. Proc Natl Acad Sci U S A 106:20464–20469 [View Article][PubMed]
    [Google Scholar]
  4. Chou S. P., Tsai C. H., Li L. Y., Liu M. Y., Chen J. Y. 2004; Characterization of monoclonal antibody to the Epstein-Barr virus BHRF1 protein, a homologue of Bcl-2. Hybrid Hybridomics 23:29–37 [View Article][PubMed]
    [Google Scholar]
  5. Danve C., Decaussin G., Busson P., Ooka T. 2001; Growth transformation of primary epithelial cells with a NPC-derived Epstein-Barr virus strain. Virology 288:223–235 [View Article][PubMed]
    [Google Scholar]
  6. Fukayama M., Chong J. M., Kaizaki Y. 1998; Epstein-Barr virus and gastric carcinoma. Gastric Cancer 1:104–114 [View Article][PubMed]
    [Google Scholar]
  7. Gargouri B., Van Pelt J., El Feki A. F., Attia H., Lassoued S. 2009; Induction of Epstein-Barr virus (EBV) lytic cycle in vitro causes oxidative stress in lymphoblastoid B cell lines. Mol Cell Biochem 324:55–63 [View Article][PubMed]
    [Google Scholar]
  8. Hammerschmidt W., Sugden B. 1989; Genetic analysis of immortalizing functions of Epstein-Barr virus in human B lymphocytes. Nature 340:393–397 [View Article][PubMed]
    [Google Scholar]
  9. Iwasaki Y., Chong J. M., Hayashi Y., Ikeno R., Arai K., Kitamura M., Koike M., Hirai K., Fukayama M. 1998; Establishment and characterization of a human Epstein-Barr virus-associated gastric carcinoma in SCID mice. J Virol 72:8321–8326[PubMed]
    [Google Scholar]
  10. Kim N., Chae H. S., Oh S. T., Kang J. H., Park C. H., Park W. S., Takada K., Lee J. M., Lee W. K., Lee S. K. 2007; Expression of viral microRNAs in Epstein-Barr virus-associated gastric carcinoma. J Virol 81:1033–1036 [View Article][PubMed]
    [Google Scholar]
  11. Kim N., Song Y. J., Lee S. K. 2011; The role of promoter methylation in Epstein-Barr virus (EBV) microRNA expression in EBV-infected B cell lines. Exp Mol Med 43:401–410 [View Article][PubMed]
    [Google Scholar]
  12. Klein G., Lindahl T., Jondal M., Leibold W., Menézes J., Nilsson K., Sundström C. 1974; Continuous lymphoid cell lines with characteristics of B cells (bone-marrow-derived), lacking the Epstein-Barr virus genome and derived from three human lymphomas. Proc Natl Acad Sci U S A 71:3283–3286 [View Article][PubMed]
    [Google Scholar]
  13. Middeldorp J. M., Brink A. A., van den Brule A. J., Meijer C. J. 2003; Pathogenic roles for Epstein-Barr virus (EBV) gene products in EBV-associated proliferative disorders. Crit Rev Oncol Hematol 45:1–36 [View Article][PubMed]
    [Google Scholar]
  14. Miller G., Lipman M. 1973; Release of infectious Epstein-Barr virus by transformed marmoset leukocytes. Proc Natl Acad Sci U S A 70:190–194 [View Article][PubMed]
    [Google Scholar]
  15. Miller G., Enders J. F., Lisco H., Kohn H. I. 1969; Establishment of lines from normal human blood leukocytes by co-cultivation with a leukocyte line derived from a leukemic child. Proc Soc Exp Biol Med 132:247–252[PubMed] [CrossRef]
    [Google Scholar]
  16. Nemerow G. R., Mold C., Schwend V. K., Tollefson V., Cooper N. R. 1987; Identification of gp350 as the viral glycoprotein mediating attachment of Epstein-Barr virus (EBV) to the EBV/C3d receptor of B cells: sequence homology of gp350 and C3 complement fragment C3d. J Virol 61:1416–1420[PubMed]
    [Google Scholar]
  17. Oh S. T., Seo J. S., Moon U. Y., Kang K. H., Shin D. J., Yoon S. K., Kim W. H., Park J. G., Lee S. K. 2004; A naturally derived gastric cancer cell line shows latency I Epstein-Barr virus infection closely resembling EBV-associated gastric cancer. Virology 320:330–336 [View Article][PubMed]
    [Google Scholar]
  18. Park J. G., Yang H. K., Kim W. H., Chung J. K., Kang M. S., Lee J. H., Oh J. H., Park H. S., Yeo K. S. other authors 1997; Establishment and characterization of human gastric carcinoma cell lines. Int J Cancer 70:443–449 [View Article][PubMed]
    [Google Scholar]
  19. Raab-Traub N., Flynn K. 1986; The structure of the termini of the Epstein-Barr virus as a marker of clonal cellular proliferation. Cell 47:883–889 [View Article][PubMed]
    [Google Scholar]
  20. Sample J., Young L., Martin B., Chatman T., Kieff E., Rickinson A., Kieff E. 1990; Epstein-Barr virus types 1 and 2 differ in their EBNA-3A, EBNA-3B, and EBNA-3C genes. J Virol 64:4084–4092[PubMed]
    [Google Scholar]
  21. Satoh T., Fukuda M., Sairenji T. 2002; Distinct patterns of mitogen-activated protein kinase phosphorylation and Epstein-Barr virus gene expression in Burkitt’s lymphoma cell lines versus B lymphoblastoid cell lines. Virus Genes 25:15–21 [View Article][PubMed]
    [Google Scholar]
  22. Shannon-Lowe C. D., Neuhierl B., Baldwin G., Rickinson A. B., Delecluse H. J. 2006; Resting B cells as a transfer vehicle for Epstein-Barr virus infection of epithelial cells. Proc Natl Acad Sci U S A 103:7065–7070 [View Article][PubMed]
    [Google Scholar]
  23. Shimizu N., Yoshiyama H., Takada K. 1996; Clonal propagation of Epstein-Barr virus (EBV) recombinants in EBV-negative Akata cells. J Virol 70:7260–7263[PubMed]
    [Google Scholar]
  24. Sugiura M., Imai S., Tokunaga M., Koizumi S., Uchizawa M., Okamoto K., Osato T. 1996; Transcriptional analysis of Epstein-Barr virus gene expression in EBV-positive gastric carcinoma: unique viral latency in the tumour cells. Br J Cancer 74:625–631 [View Article][PubMed]
    [Google Scholar]
  25. Tajima M., Komuro M., Okinaga K. 1998; Establishment of Epstein-Barr virus-positive human gastric epithelial cell lines. Jpn J Cancer Res 89:262–268 [View Article][PubMed]
    [Google Scholar]
  26. Takada K. 2000; Epstein-Barr virus and gastric carcinoma. Mol Pathol 53:255–261 [View Article][PubMed]
    [Google Scholar]
  27. Takasaka N., Tajima M., Okinaga K., Satoh Y., Hoshikawa Y., Katsumoto T., Kurata T., Sairenji T. 1998; Productive infection of Epstein-Barr virus (EBV) in EBV-genome-positive epithelial cell lines (GT38 and GT39) derived from gastric tissues. Virology 247:152–159 [View Article][PubMed]
    [Google Scholar]
  28. Teramoto N., Maeda A., Kobayashi K., Hayashi K., Oka T., Takahashi K., Takada K., Klein G., Akagi T. 2000; Epstein-Barr virus infection to Epstein-Barr virus-negative nasopharyngeal carcinoma cell line TW03 enhances its tumorigenicity. Lab Invest 80:303–312 [View Article][PubMed]
    [Google Scholar]
  29. Yoshiyama H., Shimizu N., Takada K. 1995; Persistent Epstein-Barr virus infection in a human T-cell line: unique program of latent virus expression. EMBO J 14:3706–3711[PubMed]
    [Google Scholar]
  30. Yoshiyama H., Imai S., Shimizu N., Takada K. 1997; Epstein-Barr virus infection of human gastric carcinoma cells: implication of the existence of a new virus receptor different from CD21. J Virol 71:5688–5691[PubMed]
    [Google Scholar]
  31. Young L. S., Rickinson A. B. 2004; Epstein-Barr virus: 40 years on. Nat Rev Cancer 4:757–768 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.045237-0
Loading
/content/journal/jgv/10.1099/vir.0.045237-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed