1887

Abstract

Amino acid position 123 in the E protein of Japanese encephalitis virus (JEV) determines viral growth properties and pathogenicity. The majority of JEV strains have a serine residue at this position (E); however, JEV with an asparagine residue (E) has also been isolated. To compare the growth properties and pathogenicity of E and E JEV, we produced recombinant JEV with a serine-to-asparagine substitution at position 123 (rJEV-Mie41-E) in the E-type strain Mie/41/2002 background. The growth rate of rJEV-Mie41-E was similar to that of Mie/41/2002 in mammalian and mosquito cell lines. Mouse challenge experiments showed that there was only a slight difference in neuroinvasiveness between the parent strain (Mie/41/2002) and rJEV-Mie41-E. Thus, our results indicate that the Ser-to-Asn substitution in the JEV E protein has weak impact on viral growth properties or on pathogenicity .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.044925-0
2013-01-01
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/94/1/90.html?itemId=/content/journal/jgv/10.1099/vir.0.044925-0&mimeType=html&fmt=ahah

References

  1. Aihara S. , Chunming R. , Yu Y. X. , Lee T. , Watanabe K. , Komiya T. , Sumiyoshi H. , Hashimoto H. , Nomoto A. . ( 1991; ). Identification of mutations that occurred on the genome of Japanese encephalitis virus during the attenuation process. . Virus Genes 5:, 95–109. [CrossRef] [PubMed]
    [Google Scholar]
  2. Arroyo J. , Guirakhoo F. , Fenner S. , Zhang Z. X. , Monath T. P. , Chambers T. J. . ( 2001; ). Molecular basis for attenuation of neurovirulence of a yellow fever virus/Japanese encephalitis virus chimera vaccine (ChimeriVax-JE). . J Virol 75:, 934–942. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cecilia D. , Gould E. A. . ( 1991; ). Nucleotide changes responsible for loss of neuroinvasiveness in Japanese encephalitis virus neutralization-resistant mutants. . Virology 181:, 70–77. [CrossRef] [PubMed]
    [Google Scholar]
  4. Chambers T. J. , Droll D. A. , Jiang X. , Wold W. S. , Nickells J. A. . ( 2007; ). JE Nakayama/JE SA14-14-2 virus structural region intertypic viruses: biological properties in the mouse model of neuroinvasive disease. . Virology 366:, 51–61. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chen L. K. , Liao C. L. , Lin C. G. , Lai S. C. , Liu C. I. , Ma S. H. , Huang Y. Y. , Lin Y. L. . ( 1996; ). Persistence of Japanese encephalitis virus is associated with abnormal expression of the nonstructural protein NS1 in host cells. . Virology 217:, 220–229. [CrossRef] [PubMed]
    [Google Scholar]
  6. Chen Y. Y. , Fan Y. C. , Tu W. C. , Chang R. Y. , Shih C. C. , Lu I. H. , Chien M. S. , Lee W. C. , Chen T. H. . & other authors ( 2011; ). Japanese encephalitis virus genotype replacement, Taiwan, 2009-2010. . Emerg Infect Dis 17:, 2354–2356. [CrossRef] [PubMed]
    [Google Scholar]
  7. Fulmali P. V. , Sapkal G. N. , Athawale S. , Gore M. M. , Mishra A. C. , Bondre V. P. . ( 2011; ). Introduction of Japanese encephalitis virus genotype I, India. . Emerg Infect Dis 17:, 319–321. [CrossRef] [PubMed]
    [Google Scholar]
  8. Gubler D. J. , Kuno G. , Markoff L. . ( 2007;). Flaviviruses. . In Fields Virology, , 5th edn., pp. 1153–1252. Edited by Knipe D. M. , Howley P. M. . . Philadelphila:: Lippincott Williams & Wilkins;.
    [Google Scholar]
  9. Hanna J. N. , Ritchie S. A. , Phillips D. A. , Shield J. , Bailey M. C. , Mackenzie J. S. , Poidinger M. , McCall B. J. , Mills P. J. . ( 1996; ). An outbreak of Japanese encephalitis in the Torres Strait, Australia, 1995. . Med J Aust 165:, 256–260.[PubMed]
    [Google Scholar]
  10. Hasegawa H. , Yoshida M. , Shiosaka T. , Fujita S. , Kobayashi Y. . ( 1992; ). Mutations in the envelope protein of Japanese encephalitis virus affect entry into cultured cells and virulence in mice. . Virology 191:, 158–165. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kolaskar A. S. , Kulkarni-Kale U. . ( 1999; ). Prediction of three-dimensional structure and mapping of conformational epitopes of envelope glycoprotein of Japanese encephalitis virus. . Virology 261:, 31–42. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kuwayama M. , Ito M. , Takao S. , Shimazu Y. , Fukuda S. , Miyazaki K. , Kurane I. , Takasaki T. . ( 2005; ). Japanese encephalitis virus in meningitis patients, Japan. . Emerg Infect Dis 11:, 471–473.[PubMed]
    [Google Scholar]
  13. Lee E. , Hall R. A. , Lobigs M. . ( 2004; ). Common E protein determinants for attenuation of glycosaminoglycan-binding variants of Japanese encephalitis and West Nile viruses. . J Virol 78:, 8271–8280. [CrossRef] [PubMed]
    [Google Scholar]
  14. Liang J. J. , Liao C. L. , Liao J. T. , Lee Y. L. , Lin Y. L. . ( 2009; ). A Japanese encephalitis virus vaccine candidate strain is attenuated by decreasing its interferon antagonistic ability. . Vaccine 27:, 2746–2754. [CrossRef] [PubMed]
    [Google Scholar]
  15. Lindenbach B. D. , Thiel H. J. , Rice C. M. . ( 2007; ). Flaviviridae: the viruses and their replication. . In Fields Virology, , 5th edn., pp. 1101–1151. Edited by Knipe D. M. , Howley P. M. . . Philadelphia:: Lippincott Williams & Wilkins;.
    [Google Scholar]
  16. Luca V. C. , AbiMansour J. , Nelson C. A. , Fremont D. H. . ( 2012; ). Crystal structure of the Japanese encephalitis virus envelope protein. . J Virol 86:, 2337–2346. [CrossRef] [PubMed]
    [Google Scholar]
  17. Ma S. P. , Yoshida Y. , Makino Y. , Tadano M. , Ono T. , Ogawa M. . ( 2003; ). Short report: a major genotype of Japanese encephalitis virus currently circulating in Japan. . Am J Trop Med Hyg 69:, 151–154.[PubMed]
    [Google Scholar]
  18. Monath T. P. , Arroyo J. , Levenbook I. , Zhang Z. X. , Catalan J. , Draper K. , Guirakhoo F. . ( 2002; ). Single mutation in the flavivirus envelope protein hinge region increases neurovirulence for mice and monkeys but decreases viscerotropism for monkeys: relevance to development and safety testing of live, attenuated vaccines. . J Virol 76:, 1932–1943. [CrossRef] [PubMed]
    [Google Scholar]
  19. Nam J. H. , Chung Y. J. , Ban S. J. , Kim E. J. , Park Y. K. , Cho H. W. . ( 1996; ). Envelope gene sequence variation among Japanese encephalitis viruses isolated in Korea. . Acta Virol 40:, 303–309.[PubMed]
    [Google Scholar]
  20. Nerome R. , Tajima S. , Takasaki T. , Yoshida T. , Kotaki A. , Lim C. K. , Ito M. , Sugiyama A. , Yamauchi A. . & other authors ( 2007; ). Molecular epidemiological analyses of Japanese encephalitis virus isolates from swine in Japan from 2002 to 2004. . J Gen Virol 88:, 2762–2768. [CrossRef] [PubMed]
    [Google Scholar]
  21. Nga P. T. , del Carmen Parquet M. , Cuong V. D. , Ma S. P. , Hasebe F. , Inoue S. , Makino Y. , Takagi M. , Nam V. S. , Morita K. . ( 2004; ). Shift in Japanese encephalitis virus (JEV) genotype circulating in northern Vietnam: implications for frequent introductions of JEV from Southeast Asia to East Asia. . J Gen Virol 85:, 1625–1631. [CrossRef] [PubMed]
    [Google Scholar]
  22. Ni H. , Barrett A. D. . ( 1996; ). Molecular differences between wild-type Japanese encephalitis virus strains of high and low mouse neuroinvasiveness. . J Gen Virol 77:, 1449–1455. [CrossRef] [PubMed]
    [Google Scholar]
  23. Ni H. , Barrett A. D. . ( 1998; ). Attenuation of Japanese encephalitis virus by selection of its mouse brain membrane receptor preparation escape variants. . Virology 241:, 30–36. [CrossRef] [PubMed]
    [Google Scholar]
  24. Nitatpattana N. , Dubot-Pérès A. , Gouilh M. A. , Souris M. , Barbazan P. , Yoksan S. , de Lamballerie X. , Gonzalez J. P. . ( 2008; ). Change in Japanese encephalitis virus distribution, Thailand. . Emerg Infect Dis 14:, 1762–1765. [CrossRef] [PubMed]
    [Google Scholar]
  25. Nitayaphan S. , Grant J. A. , Chang G. J. , Trent D. W. . ( 1990; ). Nucleotide sequence of the virulent SA-14 strain of Japanese encephalitis virus and its attenuated vaccine derivative, SA-14-14-2. . Virology 177:, 541–552. [CrossRef] [PubMed]
    [Google Scholar]
  26. Nybakken G. E. , Nelson C. A. , Chen B. R. , Diamond M. S. , Fremont D. H. . ( 2006; ). Crystal structure of the West Nile virus envelope glycoprotein. . J Virol 80:, 11467–11474. [CrossRef] [PubMed]
    [Google Scholar]
  27. Pan X. L. , Liu H. , Wang H. Y. , Fu S. H. , Liu H. Z. , Zhang H. L. , Li M. H. , Gao X. Y. , Wang J. L. . & other authors ( 2011; ). Emergence of genotype I of Japanese encephalitis virus as the dominant genotype in Asia. . J Virol 85:, 9847–9853. [CrossRef] [PubMed]
    [Google Scholar]
  28. Pujhari S. K. , Prabhakar S. , Ratho R. K. , Modi M. , Sharma M. , Mishra B. . ( 2010; ). A novel mutation (S227T) in domain II of the envelope gene of Japanese encephalitis virus circulating in North India. . Epidemiol Infect 139:, 849–856.[PubMed] [CrossRef]
    [Google Scholar]
  29. Rey F. A. , Heinz F. X. , Mandl C. , Kunz C. , Harrison S. C. . ( 1995; ). The envelope glycoprotein from tick-borne encephalitis virus at 2 Å resolution. . Nature 375:, 291–298. [CrossRef] [PubMed]
    [Google Scholar]
  30. Solomon T. , Ni H. , Beasley D. W. , Ekkelenkamp M. , Cardosa M. J. , Barrett A. D. . ( 2003; ). Origin and evolution of Japanese encephalitis virus in southeast Asia. . J Virol 77:, 3091–3098. [CrossRef] [PubMed]
    [Google Scholar]
  31. Sumiyoshi H. , Tignor G. H. , Shope R. E. . ( 1995; ). Characterization of a highly attenuated Japanese encephalitis virus generated from molecularly cloned cDNA. . J Infect Dis 171:, 1144–1151. [CrossRef] [PubMed]
    [Google Scholar]
  32. Tajima S. , Nerome R. , Nukui Y. , Kato F. , Takasaki T. , Kurane I. . ( 2010; ). A single mutation in the Japanese encephalitis virus E protein (S123R) increases its growth rate in mouse neuroblastoma cells and its pathogenicity in mice. . Virology 396:, 298–304. [CrossRef] [PubMed]
    [Google Scholar]
  33. Tsai T. F. . ( 2000; ). New initiatives for the control of Japanese encephalitis by vaccination: minutes of a WHO/CVI meeting, Bangkok, Thailand, 13–15 October 1998. . Vaccine 18: (Suppl. 2), 1–25. [CrossRef] [PubMed]
    [Google Scholar]
  34. Uchil P. D. , Satchidanandam V. . ( 2001; ). Phylogenetic analysis of Japanese encephalitis virus: envelope gene based analysis reveals a fifth genotype, geographic clustering, and multiple introductions of the virus into the Indian subcontinent. . Am J Trop Med Hyg 65:, 242–251.[PubMed]
    [Google Scholar]
  35. Wang H. Y. , Takasaki T. , Fu S. H. , Sun X. H. , Zhang H. L. , Wang Z. X. , Hao Z. Y. , Zhang J. K. , Tang Q. . & other authors ( 2007; ). Molecular epidemiological analysis of Japanese encephalitis virus in China. . J Gen Virol 88:, 885–894. [CrossRef] [PubMed]
    [Google Scholar]
  36. Wu S. C. , Lin C. W. , Lee S. C. , Lian W. C. . ( 2003; ). Phenotypic and genotypic characterization of the neurovirulence and neuroinvasiveness of a large-plaque attenuated Japanese encephalitis virus isolate. . Microbes Infect 5:, 475–480. [CrossRef] [PubMed]
    [Google Scholar]
  37. Wu R. , Tian Y. , Deng J. , Yang K. , Liang W. , Guo R. , Duan Z. , Liu Z. , Zhou D. , Xu D. . ( 2011; ). Multiple amino acid variations in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice. . Arch Virol 156:, 685–688. [CrossRef] [PubMed]
    [Google Scholar]
  38. Yamaguchi Y. , Nukui Y. , Tajima S. , Nerome R. , Kato F. , Watanabe H. , Takasaki T. , Kurane I. . ( 2011; ). An amino acid substitution (V3I) in the Japanese encephalitis virus NS4A protein increases its virulence in mice, but not its growth rate in vitro. . J Gen Virol 92:, 1601–1606. [CrossRef] [PubMed]
    [Google Scholar]
  39. Yang D. K. , Kim B. H. , Kweon C. H. , Kwon J. H. , Lim S. I. , Han H. R. . ( 2004; ). Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea. . J Vet Sci 5:, 197–205.[PubMed]
    [Google Scholar]
  40. Yoshida Y. , Tabei Y. , Hasegawa M. , Nagashima M. , Morozumi S. . ( 2005; ). Genotypic analysis of Japanese encephalitis virus strains isolated from swine in Tokyo, Japan. . Jpn J Infect Dis 58:, 259–261.[PubMed]
    [Google Scholar]
  41. Yun S. M. , Cho J. E. , Ju Y. R. , Kim S. Y. , Ryou J. , Han M. G. , Choi W. Y. , Jeong Y. E. . ( 2010; ). Molecular epidemiology of Japanese encephalitis virus circulating in South Korea, 1983-2005. . Virol J 7:, 127. [CrossRef] [PubMed]
    [Google Scholar]
  42. Zhang J. S. , Zhao Q. M. , Zhang P. H. , Jia N. , Cao W. C. . ( 2009; ). Genomic sequence of a Japanese encephalitis virus isolate from southern China. . Arch Virol 154:, 1177–1180. [CrossRef] [PubMed]
    [Google Scholar]
  43. Zhang J. S. , Zhao Q. M. , Guo X. F. , Zuo S. Q. , Cheng J. X. , Jia N. , Wu C. , Dai P. F. , Zhao J. Y. . ( 2011; ). Isolation and genetic characteristics of human genotype 1 Japanese encephalitis virus, China, 2009. . PLoS ONE 6:, e16418. [CrossRef] [PubMed]
    [Google Scholar]
  44. Zhao Z. , Date T. , Li Y. , Kato T. , Miyamoto M. , Yasui K. , Wakita T. . ( 2005; ). Characterization of the E-138 (Glu/Lys) mutation in Japanese encephalitis virus by using a stable, full-length, infectious cDNA clone. . J Gen Virol 86:, 2209–2220. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.044925-0
Loading
/content/journal/jgv/10.1099/vir.0.044925-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error