1887

Abstract

A Hobi-like pestivirus pair consisting of cytopathogenic (cp) and non-cytopathogenic (noncp) strains, Italy 83/10cp and Italy 83/10ncp, was isolated from the lung of a heifer that died of respiratory disease. The noncp and cp viruses were isolated on Madin–Darby bovine kidney cells and separated by plaque purification and end point dilution. Analysis of the nearly full-length genomes revealed that the two viruses were very closely related to each other and to the noncp Hobi-like strain Italy 1/10-1, which had been isolated a few weeks earlier from the same herd. One major difference between noncp and cp viruses concerned the presence of a cellular Jiv sequence in the 3′ domain of the NS2-encoding region of the cp strain. This is the first study, to our knowledge, reporting the isolation and molecular characterization of a Hobi-like virus pair.

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2012-09-01
2020-01-23
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References

  1. Avalos-Ramirez R., Orlich M., Thiel H. J., Becher P.. ( 2001;). Evidence for the presence of two novel pestivirus species. . Virology 286:, 456–465. [CrossRef][PubMed]
    [Google Scholar]
  2. Baule C., van Vuuren M., Lowings J. P., Belák S.. ( 1997;). Genetic heterogeneity of bovine viral diarrhoea viruses isolated in Southern Africa. . Virus Res 52:, 205–220. [CrossRef][PubMed]
    [Google Scholar]
  3. Becher P., Tautz N.. ( 2011;). RNA recombination in pestiviruses: cellular RNA sequences in viral genomes highlight the role of host factors for viral persistence and lethal disease. . RNA Biol 8:, 216–224. [CrossRef][PubMed]
    [Google Scholar]
  4. Becher P., Meyers G., Shannon A. D., Thiel H. J.. ( 1996;). Cytopathogenicity of border disease virus is correlated with integration of cellular sequences into the viral genome. . J Virol 70:, 2992–2998.[PubMed]
    [Google Scholar]
  5. Becher P., Orlich M., König M., Thiel H. J.. ( 1999;). Nonhomologous RNA recombination in bovine viral diarrhea virus: molecular characterization of a variety of subgenomic RNAs isolated during an outbreak of fatal mucosal disease. . J Virol 73:, 5646–5653.[PubMed]
    [Google Scholar]
  6. Becher P., Avalos Ramirez R., Orlich M., Cedillo Rosales S., König M., Schweizer M., Stalder H., Schirrmeier H., Thiel H. J.. ( 2003;). Genetic and antigenic characterization of novel pestivirus genotypes: implications for classification. . Virology 311:, 96–104. [CrossRef][PubMed]
    [Google Scholar]
  7. Boerner B., Weigelt W., Buhk H. J., Castrucci G., Ludwig H.. ( 1999;). A sensitive and specific PCR/Southern blot assay for detection of bovine herpesvirus 4 in calves infected experimentally. . J Virol Methods 83:, 169–180. [CrossRef][PubMed]
    [Google Scholar]
  8. Bolin S. R., McClurkin A. W., Cutlip R. C., Coria M. F.. ( 1985;). Severe clinical disease induced in cattle persistently infected with noncytopathic bovine viral diarrhea virus by superinfection with cytopathic bovine viral diarrhea virus. . Am J Vet Res 46:, 573–576.[PubMed]
    [Google Scholar]
  9. Brownlie J., Clarke M. C., Howard C. J.. ( 1984;). Experimental production of fatal mucosal disease in cattle. . Vet Rec 114:, 535–536. [CrossRef][PubMed]
    [Google Scholar]
  10. Buonavoglia D., Greco G., Quaranta V., Corrente M., Martella V., Decaro N.. ( 2008;). An oil-emulsion vaccine induces full-protection against Mycoplasma agalactiae infection in sheep. . New Microbiol 31:, 117–123.[PubMed]
    [Google Scholar]
  11. Buonavoglia D., Greco G., Corrente M., Greco M. F., D’Abramo M., Latronico F., Fasanella A., Decaro N.. ( 2010;). Long-term immunogenicity and protection against Mycoplasma agalactiae induced by an oil adjuvant vaccine in sheep. . Res Vet Sci 88:, 16–19. [CrossRef][PubMed]
    [Google Scholar]
  12. Couvreur B., Letellier C., Collard A., Quenon P., Dehan P., Hamers C., Pastoret P. P., Kerkhofs P.. ( 2002;). Genetic and antigenic variability in bovine viral diarrhea virus (BVDV) isolates from Belgium. . Virus Res 85:, 17–28. [CrossRef][PubMed]
    [Google Scholar]
  13. Decaro N., Elia G., Campolo M., Desario C., Mari V., Radogna A., Colaianni M. L., Cirone F., Tempesta M., Buonavoglia C.. ( 2008;). Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay. . J Virol Methods 151:, 167–171. [CrossRef][PubMed]
    [Google Scholar]
  14. Decaro N., Lucente M. S., Mari V., Cirone F., Cordioli P., Camero M., Sciarretta R., Losurdo M., Lorusso E., Buonavoglia C.. ( 2011;). Atypical pestivirus and severe respiratory disease in calves, Europe. . Emerg Infect Dis 17:, 1549–1552.[PubMed]
    [Google Scholar]
  15. Decaro N., Lucente M. S., Mari V., Sciarretta R., Pinto P., Buonavoglia D., Martella V., Buonavoglia C.. ( 2012a;). Hobi-like pestivirus in aborted bovine fetuses. . J Clin Microbiol 50:, 509–512. [CrossRef][PubMed]
    [Google Scholar]
  16. Decaro N., Mari V., Lucente M. S., Sciarretta R., Moreno A., Armenise C., Losurdo M., Camero M., Lorusso E.. & other authors ( 2012b;). Experimental infection of cattle, sheep and pigs with ‘Hobi’-like pestivirus. . Vet Microbiol 155:, 165–171. [CrossRef][PubMed]
    [Google Scholar]
  17. Decaro N., Sciarretta R., Lucente M. S., Mari V., Amorisco F., Colaianni M. L., Cordioli P., Parisi A., Lelli R., Buonavoglia C.. ( 2012c;). A nested PCR approach for unambiguous typing of pestiviruses infecting cattle. . Mol Cell Probes 26:, 42–46. [CrossRef][PubMed]
    [Google Scholar]
  18. Donis R. O., Dubovi E. J.. ( 1987;). Differences in virus-induced polypeptides in cells infected by cytopathic and noncytopathic biotypes of bovine virus diarrhea-mucosal disease virus. . Virology 158:, 168–173. [CrossRef][PubMed]
    [Google Scholar]
  19. Flores E. F., Ridpath J. F., Weiblen R., Vogel F. S., Gil L. H.. ( 2002;). Phylogenetic analysis of Brazilian bovine viral diarrhea virus type 2 (BVDV-2) isolates: evidence for a subgenotype within BVDV-2. . Virus Res 87:, 51–60. [CrossRef][PubMed]
    [Google Scholar]
  20. Hall T. A.. ( 1999;). BioEdit: a user-friendly biological sequence alignment and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  21. Hu J., Toft D. O., Seeger C.. ( 1997;). Hepadnavirus assembly and reverse transcription require a multi-component chaperone complex which is incorporated into nucleocapsids. . EMBO J 16:, 59–68. [CrossRef][PubMed]
    [Google Scholar]
  22. Kümmerer B. M., Stoll D., Meyers G.. ( 1998;). Bovine viral diarrhea virus strain Oregon: a novel mechanism for processing of NS2-3 based on point mutations. . J Virol 72:, 4127–4138.[PubMed]
    [Google Scholar]
  23. Lackner T., Müller A., Pankraz A., Becher P., Thiel H. J., Gorbalenya A. E., Tautz N.. ( 2004;). Temporal modulation of an autoprotease is crucial for replication and pathogenicity of an RNA virus. . J Virol 78:, 10765–10775. [CrossRef][PubMed]
    [Google Scholar]
  24. Lackner T., Müller A., König M., Thiel H. J., Tautz N.. ( 2005;). Persistence of bovine viral diarrhea virus is determined by a cellular cofactor of a viral autoprotease. . J Virol 79:, 9746–9755. [CrossRef][PubMed]
    [Google Scholar]
  25. Lindenbach B. D., Thiel H.-J., Rice C. M.. ( 2007;). Flaviviridae: the viruses and their replication. . In Fields Virology, , 5th edn., vol. 1, pp. 1101–1152. Edited by Knipe D. M., Howley P. M... Philadelphia, PA:: Lippincott Williams & Wilkins;.
    [Google Scholar]
  26. Liu L., Xia H., Belák S., Baule C.. ( 2008;). A TaqMan real-time RT-PCR assay for selective detection of atypical bovine pestiviruses in clinical samples and biological products. . J Virol Methods 154:, 82–85. [CrossRef][PubMed]
    [Google Scholar]
  27. Liu L., Kampa J., Belák S., Baule C.. ( 2009a;). Virus recovery and full-length sequence analysis of atypical bovine pestivirus Th/04_KhonKaen. . Vet Microbiol 138:, 62–68. [CrossRef][PubMed]
    [Google Scholar]
  28. Liu L., Xia H., Baule C., Belák S.. ( 2009b;). Maximum likelihood and Bayesian analyses of a combined nucleotide sequence dataset for genetic characterization of a novel pestivirus, SVA/cont-08. . Arch Virol 154:, 1111–1116. [CrossRef][PubMed]
    [Google Scholar]
  29. Liu L., Xia H., Wahlberg N., Belák S., Baule C.. ( 2009c;). Phylogeny, classification and evolutionary insights into pestiviruses. . Virology 385:, 351–357. [CrossRef][PubMed]
    [Google Scholar]
  30. Lyon M., Leroux C., Greenland T., Chastang J., Patet J., Mornex J. F.. ( 1997;). Presence of a unique parainfluenza virus 3 strain identified by RT-PCR in visna-maedi virus infected sheep. . Vet Microbiol 57:, 95–104. [CrossRef][PubMed]
    [Google Scholar]
  31. Meyers G., Thiel H. J.. ( 1996;). Molecular characterization of pestiviruses. . Adv Virus Res 47:, 53–118. [CrossRef][PubMed]
    [Google Scholar]
  32. Meyers G., Stoll D., Gunn M.. ( 1998;). Insertion of a sequence encoding light chain 3 of microtubule-associated proteins 1A and 1B in a pestivirus genome: connection with virus cytopathogenicity and induction of lethal disease in cattle. . J Virol 72:, 4139–4148.[PubMed]
    [Google Scholar]
  33. Neill J. D., Ridpath J. F.. ( 2001;). Recombination with a cellular mRNA encoding a novel DnaJ protein results in biotype conversion in genotype 2 bovine viral diarrhea viruses. . Virus Res 79:, 59–69. [CrossRef][PubMed]
    [Google Scholar]
  34. Peletto S., Zuccon F., Pitti M., Gobbi E., Marco L. D., Caramelli M., Masoero L., Acutis P. L.. ( 2012;). Detection and phylogenetic analysis of an atypical pestivirus, strain IZSPLV_To. . Res Vet Sci 92:, 147–150. [CrossRef][PubMed]
    [Google Scholar]
  35. Pellerin C., van den Hurk J., Lecomte J., Tijssen P.. ( 1994;). Identification of a new group of bovine viral diarrhea virus strains associated with severe outbreaks and high mortalities. . Virology 203:, 260–268. [CrossRef][PubMed]
    [Google Scholar]
  36. Peterhans E., Bachofen C., Stalder H., Schweizer M.. ( 2010;). Cytopathic bovine viral diarrhea viruses (BVDV): emerging pestiviruses doomed to extinction. . Vet Res 41:, 44. [CrossRef][PubMed]
    [Google Scholar]
  37. Pocock D. H., Howard C. J., Clarke M. C., Brownlie J.. ( 1987;). Variation in the intracellular polypeptide profiles from different isolates of bovine virus diarrhoea virus. . Arch Virol 94:, 43–53. [CrossRef][PubMed]
    [Google Scholar]
  38. Ridpath J. F., Neill J. D.. ( 2000;). Detection and characterization of genetic recombination in cytopathic type 2 bovine viral diarrhea viruses. . J Virol 74:, 8771–8774. [CrossRef][PubMed]
    [Google Scholar]
  39. Ridpath J. F., Lewis T. L., Bolin S. R., Berry E. S.. ( 1991;). Antigenic and genomic comparison between non-cytopathic and cytopathic bovine viral diarrhoea viruses isolated from cattle that had spontaneous mucosal disease. . J Gen Virol 72:, 725–729. [CrossRef][PubMed]
    [Google Scholar]
  40. Ridpath J. F., Bolin S. R., Dubovi E. J.. ( 1994;). Segregation of bovine viral diarrhea virus into genotypes. . Virology 205:, 66–74. [CrossRef][PubMed]
    [Google Scholar]
  41. Rinck G., Birghan C., Harada T., Meyers G., Thiel H. J., Tautz N.. ( 2001;). A cellular J-domain protein modulates polyprotein processing and cytopathogenicity of a pestivirus. . J Virol 75:, 9470–9482. [CrossRef][PubMed]
    [Google Scholar]
  42. Schirrmeier H., Strebelow G., Depner K., Hoffmann B., Beer M.. ( 2004;). Genetic and antigenic characterization of an atypical pestivirus isolate, a putative member of a novel pestivirus species. . J Gen Virol 85:, 3647–3652. [CrossRef][PubMed]
    [Google Scholar]
  43. Simmonds P., Becher P., Collet M. S., Gould E. A., Heinz F. X., Meyers G., Monath T., Pletnev A., Rice C. M.. & other authors ( 2011;). Family Flaviviridae. Virus Taxonomy, Ninth Report of the International Committee on Taxonomy of Viruses, pp. 1003–1020. Edited by King A. M. Q., Adams M. J., Carstens E. B., Lefkowitz E. J... Amsterdam:: Elsevier Academic Press;.
    [Google Scholar]
  44. Ståhl K., Kampa J., Alenius S., Persson Wadman A., Baule C., Aiumlamai S., Belák S.. ( 2007;). Natural infection of cattle with an atypical ‘HoBi’-like pestivirus – implications for BVD control and for the safety of biological products. . Vet Res 38:, 517–523. [CrossRef][PubMed]
    [Google Scholar]
  45. Stalder H. P., Meier P., Pfaffen G., Wageck-Canal C., Rüfenacht J., Schaller P., Bachofen C., Marti S., Vogt H. R., Peterhans E.. ( 2005;). Genetic heterogeneity of pestiviruses of ruminants in Switzerland. . Prev Vet Med 72:, 37–41, discussion 215–219. [CrossRef][PubMed]
    [Google Scholar]
  46. Sullivan D. G., Akkina R. K.. ( 1995;). A nested polymerase chain reaction assay to differentiate pestiviruses. . Virus Res 38:, 231–239. [CrossRef][PubMed]
    [Google Scholar]
  47. Tajima M.. ( 2004;). Bovine viral diarrhea virus 1 is classified into different subgenotypes depending on the analyzed region within the viral genome. . Vet Microbiol 99:, 131–138. [CrossRef][PubMed]
    [Google Scholar]
  48. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  49. Tautz N., Meyers G., Thiel H.-J.. ( 1993;). Processing of poly-ubiquitin in the polyprotein of an RNA virus. . Virology 197:, 74–85. [CrossRef][PubMed]
    [Google Scholar]
  50. Tomita Y., Mizuno T., Díez J., Naito S., Ahlquist P., Ishikawa M.. ( 2003;). Mutation of host DnaJ homolog inhibits brome mosaic virus negative-strand RNA synthesis. . J Virol 77:, 2990–2997. [CrossRef][PubMed]
    [Google Scholar]
  51. Valarcher J. F., Bourhy H., Gelfi J., Schelcher F.. ( 1999;). Evaluation of a nested reverse transcription-PCR assay based on the nucleoprotein gene for diagnosis of spontaneous and experimental bovine respiratory syncytial virus infections. . J Clin Microbiol 37:, 1858–1862.[PubMed]
    [Google Scholar]
  52. Vilcek S.. ( 1993;). Detection of the bovine herpesvirus-1 (BHV-1) genome by PCR. . J Virol Methods 41:, 245–247. [CrossRef][PubMed]
    [Google Scholar]
  53. Vilcek S., Alenius S., Paton D. J., Mittelholzer C., Belák S.. ( 1999a;). Genetic clustering of bovine viral diarrhoea viruses in cattle farms: genetic identification and analysis of viruses directly from cattle sera. . Vet J 158:, 33–38. [CrossRef][PubMed]
    [Google Scholar]
  54. Vilcek S., Drew T. W., McGoldrick A., Paton D. J.. ( 1999b;). Genetic typing of bovine pestiviruses from England and Wales. . Vet Microbiol 69:, 227–237. [CrossRef][PubMed]
    [Google Scholar]
  55. Vilcek S., Paton D., Lowings P., Björklund H., Nettleton P., Belák S.. ( 1999c;). Genetic analysis of pestiviruses at the 3′ end of the genome. . Virus Genes 18:, 107–114. [CrossRef][PubMed]
    [Google Scholar]
  56. Vilcek S., Greiser-Wilke I., Nettleton P., Paton D. J.. ( 2000;). Cellular insertions in the NS2-3 genome region of cytopathic bovine viral diarrhoea virus (BVDV) isolates. . Vet Microbiol 77:, 129–136. [CrossRef][PubMed]
    [Google Scholar]
  57. Wolfmeyer A., Wolf G., Beer M., Strube W., Hehnen H. R., Schmeer N., Kaaden O. R.. ( 1997;). Genomic (5′UTR) and serological differences among German BVDV field isolates. . Arch Virol 142:, 2049–2057. [CrossRef][PubMed]
    [Google Scholar]
  58. Xia H., Vijayaraghavan B., Belák S., Liu L.. ( 2011;). Detection and identification of the atypical bovine pestiviruses in commercial foetal bovine serum batches. . PLoS ONE 6:, e28553. [CrossRef][PubMed]
    [Google Scholar]
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