1887

Abstract

Airborne influenza virus infection of mice can be prevented by gaseous chlorine dioxide (ClO). This study demonstrated that ClO abolished the function of the haemagglutinin (HA) of influenza A virus (H1N1) in a concentration-, time- and temperature-dependent manner. The IC during a 2 min reaction with ClO at 25 °C was 13.7 µM, and the half-life time of HA with 100 µM ClO at 25 °C was 19.5 s. Peptides generated from a tryptic digest of ClO-treated virus were analysed by mass spectrometry. An HA fragment, NLLWLTGK was identified in which the tryptophan residue (W153) was 32 mass units greater than expected. The W153 residue of this peptide, which is derived from the central region of the receptor-binding site of HA, is highly conserved. It was shown that W153 was oxidized to -formylkynurenine in ClO-treated virus. It was concluded that the inactivation of influenza virus by ClO is caused by oxidation of W153 in HA, thereby abolishing its receptor-binding ability.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.044263-0
2012-12-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/93/12/2558.html?itemId=/content/journal/jgv/10.1099/vir.0.044263-0&mimeType=html&fmt=ahah

References

  1. Akamatsu A., Lee C., Morino H., Miura T., Ogata N., Shibata T.. ( 2012;). Six-month low level chlorine dioxide gas inhalation toxicity study with two-week recovery period in rats. . J Occup Med Toxicol 7:, 2. [CrossRef][PubMed]
    [Google Scholar]
  2. Biemann K.. ( 1988;). Contributions of mass spectrometry to peptide and protein structure. . Biomed Environ Mass Spectrom 16:, 99–111. [CrossRef][PubMed]
    [Google Scholar]
  3. Gamblin S. J., Skehel J. J.. ( 2010;). Influenza hemagglutinin and neuraminidase membrane glycoproteins. . J Biol Chem 285:, 28403–28409. [CrossRef][PubMed]
    [Google Scholar]
  4. Herrmann B.. ( 1978;). [Production of influenza virus recombinations]. . Arch Exp Veterinarmed 32:, 455–464 (in German).[PubMed]
    [Google Scholar]
  5. Kallin E., Lönn H., Norberg T.. ( 1986;). New derivatization and separation procedures for reducing oligosaccharides. . Glycoconj J 3:, 311–319. [CrossRef]
    [Google Scholar]
  6. Knossow M., Skehel J. J.. ( 2006;). Variation and infectivity neutralization in influenza. . Immunology 119:, 1–7. [CrossRef][PubMed]
    [Google Scholar]
  7. Lin T., Wang G., Li A., Zhang Q., Wu C., Zhang R., Cai Q., Song W., Yuen K.-Y.. ( 2009;). The hemagglutinin structure of an avian H1N1 influenza A virus. . Virology 392:, 73–81. [CrossRef][PubMed]
    [Google Scholar]
  8. Martín J., Wharton S. A., Lin Y. P., Takemoto D. K., Skehel J. J., Wiley D. C., Steinhauer D. A.. ( 1998;). Studies of the binding properties of influenza hemagglutinin receptor-site mutants. . Virology 241:, 101–111. [CrossRef][PubMed]
    [Google Scholar]
  9. Meisner J., Szretter K. J., Bradley K. C., Langley W. A., Li Z.-N., Lee B.-J., Thoennes S., Martin J., Skehel J. J.. & other authors ( 2008;). Infectivity studies of influenza virus hemagglutinin receptor binding site mutants in mice. . J Virol 82:, 5079–5083. [CrossRef][PubMed]
    [Google Scholar]
  10. Morino H., Matsubara A., Fukuda T., Shibata T.. ( 2007;). [Inhibition of hyphal growth of the fungus Alternaria alternata by chlorine dioxide gas at very low concentrations]. . Yakugaku Zasshi 127:, 773–777 (in Japanese). [CrossRef][PubMed]
    [Google Scholar]
  11. Morino H., Fukuda T., Miura T., Lee C., Shibata T., Sanekata T.. ( 2009;). Inactivation of feline calicivirus, a norovirus surrogate, by chlorine dioxide gas. . Biocontrol Sci 14:, 147–153. [CrossRef][PubMed]
    [Google Scholar]
  12. Morino H., Fukuda T., Miura T., Shibata T.. ( 2011;). Effect of low-concentration chlorine dioxide gas against bacteria and viruses on a glass surface in wet environments. . Lett Appl Microbiol 53:, 628–634. [CrossRef][PubMed]
    [Google Scholar]
  13. Napolitano M. J., Green B. J., Nicoson J. S., Margerum D. W.. ( 2005;). Chlorine dioxide oxidations of tyrosine, N-acetyltyrosine, and dopa. . Chem Res Toxicol 18:, 501–508. [CrossRef][PubMed]
    [Google Scholar]
  14. Ogata N.. ( 2007;). Denaturation of protein by chlorine dioxide: oxidative modification of tryptophan and tyrosine residues. . Biochemistry 46:, 4898–4911. [CrossRef][PubMed]
    [Google Scholar]
  15. Ogata N., Shibata T.. ( 2008;). Protective effect of low-concentration chlorine dioxide gas against influenza A virus infection. . J Gen Virol 89:, 60–67. [CrossRef][PubMed]
    [Google Scholar]
  16. Ogata N., Shibata T.. ( 2009;). Effect of chlorine dioxide gas of extremely low concentration on absenteeism of schoolchildren. . Int J Med Med Sci 1:, 288–289.
    [Google Scholar]
  17. Sanekata T., Fukuda T., Miura T., Morino H., Lee C., Maeda K., Araki K., Otake T., Kawahata T., Shibata T.. ( 2010;). Evaluation of the antiviral activity of chlorine dioxide and sodium hypochlorite against feline calicivirus, human influenza virus, measles virus, canine distemper virus, human herpesvirus, human adenovirus, canine adenovirus and canine parvovirus. . Biocontrol Sci 15:, 45–49. [CrossRef][PubMed]
    [Google Scholar]
  18. Sauter N. K., Glick G. D., Crowther R. L., Park S. J., Eisen M. B., Skehel J. J., Knowles J. R., Wiley D. C.. ( 1992;). Crystallographic detection of a second ligand binding site in influenza virus hemagglutinin. . Proc Natl Acad Sci U S A 89:, 324–328. [CrossRef][PubMed]
    [Google Scholar]
  19. Skehel J. J., Wiley D. C.. ( 2000;). Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin. . Annu Rev Biochem 69:, 531–569. [CrossRef][PubMed]
    [Google Scholar]
  20. Stevens J., Corper A. L., Basler C. F., Taubenberger J. K., Palese P., Wilson I. A.. ( 2004;). Structure of the uncleaved human H1 hemagglutinin from the extinct 1918 influenza virus. . Science 303:, 1866–1870. [CrossRef][PubMed]
    [Google Scholar]
  21. Stevens J., Blixt O., Tumpey T. M., Taubenberger J. K., Paulson J. C., Wilson I. A.. ( 2006;). Structure and receptor specificity of the hemagglutinin from an H5N1 influenza virus. . Science 312:, 404–410. [CrossRef][PubMed]
    [Google Scholar]
  22. Stewart D. J., Napolitano M. J., Bakhmutova-Albert E. V., Margerum D. W.. ( 2008;). Kinetics and mechanisms of chlorine dioxide oxidation of tryptophan. . Inorg Chem 47:, 1639–1647. [CrossRef][PubMed]
    [Google Scholar]
  23. Xie Y., Gong J., Li M., Fang H., Xu W.. ( 2011;). The medicinal potential of influenza virus surface proteins: hemagglutinin and neuraminidase. . Curr Med Chem 18:, 1050–1066. [CrossRef][PubMed]
    [Google Scholar]
  24. Yang Z.-Y., Wei C.-J., Kong W.-P., Wu L., Xu L., Smith D. F., Nabel G. J.. ( 2007;). Immunization by avian H5 influenza hemagglutinin mutants with altered receptor binding specificity. . Science 317:, 825–828. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.044263-0
Loading
/content/journal/jgv/10.1099/vir.0.044263-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error