1887

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Volume 93, Issue 11

Respiratory syncytial virus modifies microRNAs regulating host genes that affect virus replication Open Access

Abstract

Respiratory syncytial virus (RSV) causes substantial morbidity and life-threatening lower respiratory tract disease in infants, young children and the elderly. Understanding the host response to RSV infection is critical for developing disease-intervention approaches. The role of microRNAs (miRNAs) in post-transcriptional regulation of host genes responding to RSV infection is not well understood. In this study, it was shown that RSV infection of a human alveolar epithelial cell line (A549) induced five miRNAs (let-7f, miR-24, miR-337-3p, miR-26b and miR-520a-5p) and repressed two miRNAs (miR-198 and miR-595), and showed that RSV G protein triggered let-7f expression. Luciferase–untranslated region reporters and miRNA mimics and inhibitors validated the predicted targets, which included cell-cycle genes (, and ), a chemokine gene () and the suppressor of cytokine signalling 3 gene (). Modulating let-7 family miRNA levels with miRNA mimics and inhibitors affected RSV replication, indicating that RSV modulates host miRNA expression to affect the outcome of the antiviral host response, and this was mediated in part through RSV G protein expression.

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© 2012 SGM
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2012-11-01
2024-04-19
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Respiratory syncytial virus modifies microRNAs regulating host genes that affect virus replication
J. Gen. Virol. 93, 2346 (2012); https://doi.org/10.1099/vir.0.044255-0
/content/journal/jgv/10.1099/vir.0.044255-0
/content/journal/jgv/10.1099/vir.0.044255-0
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