1887

Abstract

A baculovirus, named BomaNPV S2, was isolated from a diseased larva of the wild silkworm, . Notably, BomaNPV S2 exhibited a distinguishing feature in that its host range covered that of both nucleopolyhedrosis virus (BmNPV) and multiple nucleopolyhedrovirus (AcMNPV) in cultured cells. It could replicate in cells of (5 and N), (9) and (-5B1-4). However, occlusion-derived virions of BomaNPV S2 in cells contained only a single nucleocapsid, whereas they contained multiple nucleocapsids in -5B1-4 cells. The complete genome sequence of BomaNPV S2, including predicted ORFs, was determined and compared with the genome sequence of its close relatives. The comparison results showed that most of the BomaNPV S2 genome sequence was shared with BmNPV (BmNPV T3) or BomaNPV S1, but several regions seemed more similar to regions of AcMNPV. This observation might explain why BomaNPV S2 covers the host ranges of BmNPV and AcMNPV. Further recombinant virus infection experiments demonstrated that GP64 plays an important role in BomaNPV S2 host-range determination.

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2012-11-01
2020-01-24
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