RT Journal Article SR Electronic(1) A1 Mishra, Charitra Kumar A1 Choi, Tae Jin A1 Kang, Sun ChulYR 2012 T1 Isolation and characterization of a bacteriophage F20 virulent to Enterobacter aerogenes JF Journal of General Virology, VO 93 IS 10 SP 2310 OP 2314 DO https://doi.org/10.1099/vir.0.043562-0 PB Microbiology Society, SN 1465-2099, AB An aquatic phage, designated F20, was characterized and its physico-chemical characteristics studied. F20 was specifically virulent to only two strains of Enterobacter aerogenes (ATCC 13048 and the multi-drug-resistant strain K113) among other species tested (n = 15). It was classified in the family Siphoviridae of T1-like viruses and contained a linear dsDNA genome estimated to be 51.5 kbp enclosed by an isometric capsid of 50±2 nm in diameter and a tail of 150±3 nm in length. F20 was able to survive in a broad pH range between 4 and 11, showed potential for future animal trials using oral solution and resisted chloroform and ethanol. It exhibited remarkable stability between room temperature and 70 °C for up to 150 min, and even up to 6 months at room temperature. Knowledge of this phage belonging to the widespread T1-like viruses might be helpful for adopting therapeutic strategies against E. aerogenes., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.043562-0