1887

Abstract

An aquatic phage, designated F20, was characterized and its physico-chemical characteristics studied. F20 was specifically virulent to only two strains of (ATCC 13048 and the multi-drug-resistant strain K113) among other species tested ( = 15). It was classified in the family of T1-like viruses and contained a linear dsDNA genome estimated to be 51.5 kbp enclosed by an isometric capsid of 50±2 nm in diameter and a tail of 150±3 nm in length. F20 was able to survive in a broad pH range between 4 and 11, showed potential for future animal trials using oral solution and resisted chloroform and ethanol. It exhibited remarkable stability between room temperature and 70 °C for up to 150 min, and even up to 6 months at room temperature. Knowledge of this phage belonging to the widespread T1-like viruses might be helpful for adopting therapeutic strategies against .

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2012-10-01
2020-07-07
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References

  1. Adams M. H. 1969 Discovery of Bacteriophages, and Study of Bacterial Virus, Bacteriophages New York: Interscience;
    [Google Scholar]
  2. Alekshun M. N., Levy S. B. 2007; Molecular mechanisms of antibacterial multidrug resistance. Cell 128:1037–1050 [CrossRef][PubMed]
    [Google Scholar]
  3. Bornet C., Davin-Regli A., Bosi C., Pages J. M., Bollet C. 2000; Imipenem resistance of Enterobacter aerogenes mediated by outer membrane permeability. J Clin Microbiol 38:1048–1052[PubMed]
    [Google Scholar]
  4. CDC 1999; National Nosocomial Infections Surveillance (NNIS) System report, data summary from January 1990–May 1999, issued June 1999. Am J Infect Control 27:520–532 [CrossRef][PubMed]
    [Google Scholar]
  5. Duckworth D. H. 1976; “Who discovered bacteriophage?”. Bacteriol Rev 40:793–802[PubMed]
    [Google Scholar]
  6. Durmaz E., Klaenhammer T. R. 2000; Genetic analysis of chromosomal regions of Lactococcus lactis acquired by recombinant lytic phages. Appl Environ Microbiol 66:895–903 [CrossRef][PubMed]
    [Google Scholar]
  7. Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. 2005 Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses Amsterdam: Elsevier Academic Press;
    [Google Scholar]
  8. Jamalludeen N., Johnson R. P., Friendship R., Kropinski A. M., Lingohr E. J., Gyles C. L. 2007; Isolation and characterization of nine bacteriophages that lyse O149 enterotoxigenic Escherichia coli . Vet Microbiol 124:47–57 [CrossRef][PubMed]
    [Google Scholar]
  9. Lin L., Hong W., Ji X., Han J., Huang L., Wei Y. 2010; Isolation and characterization of an extremely long tail Thermus bacteriophage from Tengchong hot springs in China. J Basic Microbiol 50:452–456 [CrossRef][PubMed]
    [Google Scholar]
  10. Loessner M. J., Neugirg E., Zink R., Scherer S. 1993; Isolation, classification and molecular characterization of bacteriophages for Enterobacter species. J Gen Microbiol 139:2627–2633 [CrossRef][PubMed]
    [Google Scholar]
  11. Lu Z., Breidt F. Jr, Fleming H. P., Altermann E., Klaenhammer T. R. 2003; Isolation and characterization of a Lactobacillus plantarum bacteriophage, ΦJL-1, from a cucumber fermentation. Int J Food Microbiol 84:225–235 [CrossRef][PubMed]
    [Google Scholar]
  12. Marcos M., Iñurrieta A., Soriano A., Martínez J. A., Almela M., Marco F., Mensa J. 2008; Effect of antimicrobial therapy on mortality in 377 episodes of Enterobacter spp. bacteraemia. J Antimicrob Chemother 62:397–403 [CrossRef][PubMed]
    [Google Scholar]
  13. Piagnerelli M., Kennes B., Brogniez Y., Deplano A., Govaerts D. 2000; Outbreak of nosocomial multidrug-resistant Enterobacter aerogenes in a geriatric unit: failure of isolation contact, analysis of risk factors, and use of pulsed-field gel electrophoresis. Infect Control Hosp Epidemiol 21:651–653 [CrossRef][PubMed]
    [Google Scholar]
  14. Roberts M. D. 2001; T1-like viruses. In The Springer Index of Viruses pp. 1–10 Edited by Tidona C. A., Darai G. Heidelberg, Germany: Springer-Verlag Heidelberg;
    [Google Scholar]
  15. Roberts R. J., Vincze T., Posfai J., Macelis D. 2005; REBASE–restriction enzymes and DNA methyltransferases. Nucleic Acids Res 33:Database issueD230–D232 [CrossRef][PubMed]
    [Google Scholar]
  16. SCIC 1999; Guidelines for the Management of Patients with Vancomycin-Resistant Enterococci (VRE) Colonisation/Infection, Department of Human Services, MELBOURNE VIC. Standing Committee on Infection Control. http://docs.health.vic.gov.au/docs/doc/9098A54040CFBBBCCA2578CE00088FED/$FILE/guidelines_vre.pdf
  17. Sulakvelidze A., Alavidze Z., Morris J. G. Jr 2001; Bacteriophage therapy. Antimicrob Agents Chemother 45:649–659 [CrossRef][PubMed]
    [Google Scholar]
  18. Summers W. C. 2001; Bacteriophage therapy. Annu Rev Microbiol 55:437–451 [CrossRef][PubMed]
    [Google Scholar]
  19. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef][PubMed]
    [Google Scholar]
  20. US Food and Drug Administration 2006; Food additives permitted for direct addition to food for human consumption; bacteriophage preparation. Fed Regist 71:47729–47732
    [Google Scholar]
  21. Verthe K., Possemiers S., Boon N., Vaneechoutte M., Verstraete W. . ( 2004; Stability and activity of an Enterobacter aerogenes-specific bacteriophage under simulated gastro-intestinal conditions. Appl Microbiol Biotechnol 65:465–472 [CrossRef]
    [Google Scholar]
  22. Wang S., Kong J., Gao C., Guo T., Liu X. 2010; Isolation and characterization of a novel virulent phage (phiLdb) of Lactobacillus delbrueckii . Int J Food Microbiol 137:22–27 [CrossRef][PubMed]
    [Google Scholar]
  23. Wright G. D. 2007; The antibiotic resistome: the nexus of chemical and genetic diversity. Nat Rev Microbiol 5:175–186 [CrossRef][PubMed]
    [Google Scholar]
  24. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744 [CrossRef][PubMed]
    [Google Scholar]
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