Characterization of the bacteriophage vB_SenS-Ent1 Free

Abstract

The bacteriophage vB_SenS-Ent1 (Ent1) is a member of the family of tailed bacteriophages and infects a broad range of serovars of the enteric pathogen . The virion particle is composed of an icosahedral head 64 nm in diameter and a flexible, non-contractile tail of 116 × 8.5 nm possessing terminal fibres. The adsorption rate constant at 37 °C is 6.73 × 10 ml min. Latent and eclipse periods are 25 and 20 min, respectively, and the burst size is 35 progeny particles per cell after 35 min at 37 °C. Sequencing revealed a circularly permuted, 42 391 bp dsDNA genome containing 58 ORFs organized into four major transcriptional units. Comparisons with the genome sequences of other bacteriophages revealed a high level of nucleotide sequence identity and shared orthologous proteins with the phages SETP3, SE2 and KS7 (SS3e) and the phages K1G, K1H, K1ind1 and K1ind3.

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2012-09-01
2024-03-29
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