Group A rotavirus (RVA) strain K8 (RVA/Human-tc/JPN/K8/1977/G1P[9]) was found to have Wa-like VP7 and NSP1 genes and AU-1-like VP4 and NSP5 genes. To determine the exact origin and overall genetic makeup of this unusual RVA strain, the remaining genes (VP1–VP3, VP6 and NSP2–NSP4) of K8 were analysed in this study. Strain K8 exhibited a G1-P[9]-I1-R3-C3-M3-A1-N1-T3-E3-H3 genotype constellation, not reported previously. The VP6 and NSP2 genes of strain K8 were related closely to those of common human Wa-like G1P[8] and/or G3P[8] strains, whilst its VP1–VP3, NSP3 and NSP4 genes were related more closely to those of AU-1-like RVAs and/or AU-1-like genes of multi-reassortant strains than to those of other RVAs. Therefore, strain K8 might have originated from intergenogroup-reassortment events involving acquisition of four Wa-like genes, possibly from G1P[8] RVAs, by an AU-1-like P[9] strain. Whole-genomic analysis of strain K8 has provided important insights into the complex genetic diversity of RVAs.
BonkoungouI. J., DamankaS., SanouI., TiendrébéogoF., CoulibalyS. O., BonF., HaukkaK., TraoréA. S., BarroN., ArmahG. E.2011; Genotype diversity of group A rotavirus strains in children with acute diarrhea in urban Burkina Faso, 2008–2010. J Med Virol 83:1485–1490 [View Article][PubMed]
CashmanO., CollinsP. J., LennonG., CryanB., MartellaV., FanningS., StainesA., O’SheaH.2012; Molecular characterization of group A rotaviruses detected in children with gastroenteritis in Ireland in 2006–2009. Epidemiol Infect 140:247–259 [View Article][PubMed]
EstesM. K., KapikianA. Z.2007; Rotaviruses and their replication. In Fields Virology, 5th edn. pp. 1917–1974 Edited by FieldsB. N., KnipeD. M., HowleyP. M., GriffinD. E., LambR. A., MartinM. A., RoizmanB., StrausS. E. Philadelphia, PA: Lippincott Williams & Wilkins;
GhoshS., KobayashiN.2011; Whole-genomic analysis of rotavirus strains: current status and future prospects. Future Microbiol 6:1049–1065 [View Article][PubMed]
GhoshS., AlamM. M., AhmedM. U., TalukdarR. I., PaulS. K., KobayashiN.2010a; Complete genome constellation of a caprine group A rotavirus strain reveals common evolution with ruminant and human rotavirus strains. J Gen Virol 91:2367–2373 [View Article][PubMed]
HeimanE. M., McDonaldS. M., BarroM., TaraporewalaZ. F., Bar-MagenT., PattonJ. T.2008; Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions. J Virol 82:11106–11116 [View Article][PubMed]
LeV. P., KimJ. Y., ChoS. L., NamS. W., LimI., LeeH. J., KimK., ChungS. I., SongW.other authors2008; Detection of unusual rotavirus genotypes G8P[8] and G12P[6] in South Korea. J Med Virol 80:175–182 [View Article][PubMed]
LeiteJ. P., AlfieriA. A., WoodsP. A., GlassR. I., GentschJ. R.1996; Rotavirus G and P types circulating in Brazil: characterization by RT-PCR, probe hybridization, and sequence analysis. Arch Virol 141:2365–2374 [View Article][PubMed]
MartellaV., PotgieterA. C., LorussoE., De GraziaS., GiammancoG. M., MatthijnssensJ., BányaiK., CiarletM., LavazzaA.other authors2011; A feline rotavirus G3P[9] carries traces of multiple reassortment events and resembles rare human G3P[9] rotaviruses. J Gen Virol 92:1214–1221 [View Article][PubMed]
MatthijnssensJ., CiarletM., HeimanE., ArijsI., DelbekeT., McDonaldS. M., PalomboE. A., Iturriza-GómaraM., MaesP.other authors2008a; Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. J Virol 82:3204–3219 [View Article][PubMed]
MatthijnssensJ., CiarletM., RahmanM., AttouiH., BányaiK., EstesM. K., GentschJ. R., Iturriza-GómaraM., KirkwoodC. D.other authors2008b; Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. Arch Virol 153:1621–1629 [View Article][PubMed]
MatthijnssensJ., HeylenE., ZellerM., RahmanM., LemeyP., Van RanstM.2010a; Phylodynamic analyses of rotavirus genotypes G9 and G12 underscore their potential for swift global spread. Mol Biol Evol 27:2431–2436 [View Article][PubMed]
MatthijnssensJ., CiarletM., McDonaldS. M., AttouiH., BányaiK., BristerJ. R., BuesaJ., EsonaM. D., EstesM. K.other authors2011a; Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). Arch Virol 156:1397–1413 [View Article][PubMed]
MatthijnssensJ., De GraziaS., PiessensJ., HeylenE., ZellerM., GiammancoG. M., BányaiK., BuonavogliaC., CiarletM.other authors2011b; Multiple reassortment and interspecies transmission events contribute to the diversity of feline, canine and feline/canine-like human group A rotavirus strains. Infect Genet Evol 11:1396–1406 [View Article][PubMed]
McDonaldS. M., MatthijnssensJ., McAllenJ. K., HineE., OvertonL., WangS., LemeyP., ZellerM., Van RanstM.other authors2009; Evolutionary dynamics of human rotaviruses: balancing reassortment with preferred genome constellations. PLoS Pathog 5:e1000634 [View Article][PubMed]
NakagomiT., NakagomiO.1989; RNA–RNA hybridization identifies a human rotavirus that is genetically related to feline rotavirus. J Virol 63:1431–1434[PubMed]
NakagomiO., KagaE., NakagomiT.1992; Human rotavirus strain with unique VP4 neutralization epitopes as a result of natural reassortment between members of the AU-1 and Wa genogroups. Arch Virol 127:365–371 [View Article][PubMed]
RahmanM., MatthijnssensJ., YangX., DelbekeT., ArijsI., TaniguchiK., Iturriza-GómaraM., IftekharuddinN., AzimT., Van RanstM.2007; Evolutionary history and global spread of the emerging g12 human rotaviruses. J Virol 81:2382–2390 [View Article][PubMed]
SantosN., HoshinoY.2005; Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol 15:29–56 [View Article][PubMed]
SantosN., SoaresC. C., VolotãoE. M., AlbuquerqueM. C., HoshinoY.2003; Surveillance of rotavirus strains in Rio de Janeiro, Brazil, from 1997 to 1999. J Clin Microbiol 41:3399–3402 [View Article][PubMed]
TaniguchiK., NishikawaK., UrasawaT., UrasawaS., MidthunK., KapikianA. Z., GorzigliaM.1989; Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus (strain K8) which has unique VP4 neutralization epitopes. J Virol 63:4101–4106[PubMed]
UrasawaS., UrasawaT., TaniguchiK., ChibaS.1984; Serotype determination of human rotavirus isolates and antibody prevalence in pediatric population in Hokkaido, Japan. Arch Virol 81:1–12 [View Article][PubMed]
VillenaC., El-SenousyW. M., AbadF. X., PintóR. M., BoschA.2003; Group A rotavirus in sewage samples from Barcelona and Cairo: emergence of unusual genotypes. Appl Environ Microbiol 69:3919–3923 [View Article][PubMed]
WuH., TaniguchiK., UrasawaT., UrasawaS.1998; Serological and genomic characterization of human rotaviruses detected in China. J Med Virol 55:168–176 [View Article][PubMed]
YamamotoD., KawaguchiyaM., GhoshS., IchikawaM., NumazakiK., KobayashiN.2011; Detection and full genomic analysis of G6P[9] human rotavirus in Japan. Virus Genes 43:215–223 [View Article][PubMed]