1887

Abstract

Group A rotavirus (RVA) strain K8 (RVA/Human-tc/JPN/K8/1977/G1P[9]) was found to have Wa-like VP7 and NSP1 genes and AU-1-like VP4 and NSP5 genes. To determine the exact origin and overall genetic makeup of this unusual RVA strain, the remaining genes (VP1–VP3, VP6 and NSP2–NSP4) of K8 were analysed in this study. Strain K8 exhibited a G1-P[9]-I1-R3-C3-M3-A1-N1-T3-E3-H3 genotype constellation, not reported previously. The VP6 and NSP2 genes of strain K8 were related closely to those of common human Wa-like G1P[8] and/or G3P[8] strains, whilst its VP1–VP3, NSP3 and NSP4 genes were related more closely to those of AU-1-like RVAs and/or AU-1-like genes of multi-reassortant strains than to those of other RVAs. Therefore, strain K8 might have originated from intergenogroup-reassortment events involving acquisition of four Wa-like genes, possibly from G1P[8] RVAs, by an AU-1-like P[9] strain. Whole-genomic analysis of strain K8 has provided important insights into the complex genetic diversity of RVAs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.043026-0
2012-08-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/93/8/1700.html?itemId=/content/journal/jgv/10.1099/vir.0.043026-0&mimeType=html&fmt=ahah

References

  1. Bonkoungou I. J., Damanka S., Sanou I., Tiendrébéogo F., Coulibaly S. O., Bon F., Haukka K., Traoré A. S., Barro N., Armah G. E.. ( 2011;). Genotype diversity of group A rotavirus strains in children with acute diarrhea in urban Burkina Faso, 2008–2010. . J Med Virol 83:, 1485–1490. [CrossRef][PubMed]
    [Google Scholar]
  2. Cashman O., Collins P. J., Lennon G., Cryan B., Martella V., Fanning S., Staines A., O’Shea H.. ( 2012;). Molecular characterization of group A rotaviruses detected in children with gastroenteritis in Ireland in 2006–2009. . Epidemiol Infect 140:, 247–259. [CrossRef][PubMed]
    [Google Scholar]
  3. Estes M. K., Kapikian A. Z.. ( 2007;). Rotaviruses and their replication. . In Fields Virology, , 5th edn., pp. 1917–1974. Edited by Fields B. N., Knipe D. M., Howley P. M., Griffin D. E., Lamb R. A., Martin M. A., Roizman B., Straus S. E... Philadelphia, PA:: Lippincott Williams & Wilkins;.
    [Google Scholar]
  4. Fang Z. Y., Yang H., Qi J., Zhang J., Sun L. W., Tang J. Y., Ma L., Du Z. Q., He A. H.. & other authors ( 2002;). Diversity of rotavirus strains among children with acute diarrhea in China: 1998–2000 surveillance study. . J Clin Microbiol 40:, 1875–1878. [CrossRef][PubMed]
    [Google Scholar]
  5. Ghosh S., Kobayashi N.. ( 2011;). Whole-genomic analysis of rotavirus strains: current status and future prospects. . Future Microbiol 6:, 1049–1065. [CrossRef][PubMed]
    [Google Scholar]
  6. Ghosh S., Alam M. M., Ahmed M. U., Talukdar R. I., Paul S. K., Kobayashi N.. ( 2010a;). Complete genome constellation of a caprine group A rotavirus strain reveals common evolution with ruminant and human rotavirus strains. . J Gen Virol 91:, 2367–2373. [CrossRef][PubMed]
    [Google Scholar]
  7. Ghosh S., Kobayashi N., Nagashima S., Chawla-Sarkar M., Krishnan T., Ganesh B., Naik T. N.. ( 2010b;). Full genomic analysis and possible origin of a porcine G12 rotavirus strain RU172. . Virus Genes 40:, 382–388. [CrossRef][PubMed]
    [Google Scholar]
  8. Ghosh S., Adachi N., Gatheru Z., Nyangao J., Yamamoto D., Ishino M., Urushibara N., Kobayashi N.. ( 2011;). Whole-genome analysis reveals the complex evolutionary dynamics of Kenyan G2P[4] human rotavirus strains. . J Gen Virol 92:, 2201–2208. [CrossRef][PubMed]
    [Google Scholar]
  9. Grassi T., Bagordo F., Cavallaro A., Guido M., Malaventura C., Gabutti G., De Donno A.. ( 2012;). Sequence analysis of human rotavirus strains: comparison of clinical isolates from Northern and Southern Italy. . Eur J Clin Microbiol Infect Dis 31:, 575–582. [CrossRef][PubMed]
    [Google Scholar]
  10. Heiman E. M., McDonald S. M., Barro M., Taraporewala Z. F., Bar-Magen T., Patton J. T.. ( 2008;). Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions. . J Virol 82:, 11106–11116. [CrossRef][PubMed]
    [Google Scholar]
  11. Kojima K., Taniguchi K., Kobayashi N.. ( 1996;). Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, bovine, feline, and equine rotavirus strains. . Arch Virol 141:, 1–12. [CrossRef][PubMed]
    [Google Scholar]
  12. Le V. P., Kim J. Y., Cho S. L., Nam S. W., Lim I., Lee H. J., Kim K., Chung S. I., Song W.. & other authors ( 2008;). Detection of unusual rotavirus genotypes G8P[8] and G12P[6] in South Korea. . J Med Virol 80:, 175–182. [CrossRef][PubMed]
    [Google Scholar]
  13. Leite J. P., Alfieri A. A., Woods P. A., Glass R. I., Gentsch J. R.. ( 1996;). Rotavirus G and P types circulating in Brazil: characterization by RT-PCR, probe hybridization, and sequence analysis. . Arch Virol 141:, 2365–2374. [CrossRef][PubMed]
    [Google Scholar]
  14. Martella V., Potgieter A. C., Lorusso E., De Grazia S., Giammanco G. M., Matthijnssens J., Bányai K., Ciarlet M., Lavazza A.. & other authors ( 2011;). A feline rotavirus G3P[9] carries traces of multiple reassortment events and resembles rare human G3P[9] rotaviruses. . J Gen Virol 92:, 1214–1221. [CrossRef][PubMed]
    [Google Scholar]
  15. Matthijnssens J., Ciarlet M., Heiman E., Arijs I., Delbeke T., McDonald S. M., Palombo E. A., Iturriza-Gómara M., Maes P.. & other authors ( 2008a;). Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. . J Virol 82:, 3204–3219. [CrossRef][PubMed]
    [Google Scholar]
  16. Matthijnssens J., Ciarlet M., Rahman M., Attoui H., Bányai K., Estes M. K., Gentsch J. R., Iturriza-Gómara M., Kirkwood C. D.. & other authors ( 2008b;). Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. . Arch Virol 153:, 1621–1629. [CrossRef][PubMed]
    [Google Scholar]
  17. Matthijnssens J., Bilcke J., Ciarlet M., Martella V., Bányai K., Rahman M., Zeller M., Beutels P., Van Damme P., Van Ranst M.. ( 2009;). Rotavirus disease and vaccination: impact on genotype diversity. . Future Microbiol 4:, 1303–1316. [CrossRef][PubMed]
    [Google Scholar]
  18. Matthijnssens J., Heylen E., Zeller M., Rahman M., Lemey P., Van Ranst M.. ( 2010a;). Phylodynamic analyses of rotavirus genotypes G9 and G12 underscore their potential for swift global spread. . Mol Biol Evol 27:, 2431–2436. [CrossRef][PubMed]
    [Google Scholar]
  19. Matthijnssens J., Taraporewala Z. F., Yang H., Rao S., Yuan L., Cao D., Hoshino Y., Mertens P. P., Carner G. R.. & other authors ( 2010b;). Simian rotaviruses possess divergent gene constellations that originated from interspecies transmission and reassortment. . J Virol 84:, 2013–2026. [CrossRef][PubMed]
    [Google Scholar]
  20. Matthijnssens J., Ciarlet M., McDonald S. M., Attoui H., Bányai K., Brister J. R., Buesa J., Esona M. D., Estes M. K.. & other authors ( 2011a;). Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). . Arch Virol 156:, 1397–1413. [CrossRef][PubMed]
    [Google Scholar]
  21. Matthijnssens J., De Grazia S., Piessens J., Heylen E., Zeller M., Giammanco G. M., Bányai K., Buonavoglia C., Ciarlet M.. & other authors ( 2011b;). Multiple reassortment and interspecies transmission events contribute to the diversity of feline, canine and feline/canine-like human group A rotavirus strains. . Infect Genet Evol 11:, 1396–1406. [CrossRef][PubMed]
    [Google Scholar]
  22. McDonald S. M., Matthijnssens J., McAllen J. K., Hine E., Overton L., Wang S., Lemey P., Zeller M., Van Ranst M.. & other authors ( 2009;). Evolutionary dynamics of human rotaviruses: balancing reassortment with preferred genome constellations. . PLoS Pathog 5:, e1000634. [CrossRef][PubMed]
    [Google Scholar]
  23. Nakagomi T., Nakagomi O.. ( 1989;). RNA–RNA hybridization identifies a human rotavirus that is genetically related to feline rotavirus. . J Virol 63:, 1431–1434.[PubMed]
    [Google Scholar]
  24. Nakagomi O., Nakagomi T., Akatani K., Ikegami N.. ( 1989;). Identification of rotavirus genogroups by RNA–RNA hybridization. . Mol Cell Probes 3:, 251–261. [CrossRef][PubMed]
    [Google Scholar]
  25. Nakagomi O., Kaga E., Nakagomi T.. ( 1992;). Human rotavirus strain with unique VP4 neutralization epitopes as a result of natural reassortment between members of the AU-1 and Wa genogroups. . Arch Virol 127:, 365–371. [CrossRef][PubMed]
    [Google Scholar]
  26. Rahman M., Matthijnssens J., Yang X., Delbeke T., Arijs I., Taniguchi K., Iturriza-Gómara M., Iftekharuddin N., Azim T., Van Ranst M.. ( 2007;). Evolutionary history and global spread of the emerging g12 human rotaviruses. . J Virol 81:, 2382–2390. [CrossRef][PubMed]
    [Google Scholar]
  27. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  28. Santos N., Hoshino Y.. ( 2005;). Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. . Rev Med Virol 15:, 29–56. [CrossRef][PubMed]
    [Google Scholar]
  29. Santos N., Soares C. C., Volotão E. M., Albuquerque M. C., Hoshino Y.. ( 2003;). Surveillance of rotavirus strains in Rio de Janeiro, Brazil, from 1997 to 1999. . J Clin Microbiol 41:, 3399–3402. [CrossRef][PubMed]
    [Google Scholar]
  30. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  31. Taniguchi K., Nishikawa K., Urasawa T., Urasawa S., Midthun K., Kapikian A. Z., Gorziglia M.. ( 1989;). Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus (strain K8) which has unique VP4 neutralization epitopes. . J Virol 63:, 4101–4106.[PubMed]
    [Google Scholar]
  32. Urasawa S., Urasawa T., Taniguchi K., Chiba S.. ( 1984;). Serotype determination of human rotavirus isolates and antibody prevalence in pediatric population in Hokkaido, Japan. . Arch Virol 81:, 1–12. [CrossRef][PubMed]
    [Google Scholar]
  33. Villena C., El-Senousy W. M., Abad F. X., Pintó R. M., Bosch A.. ( 2003;). Group A rotavirus in sewage samples from Barcelona and Cairo: emergence of unusual genotypes. . Appl Environ Microbiol 69:, 3919–3923. [CrossRef][PubMed]
    [Google Scholar]
  34. Wang Y. H., Kobayashi N., Nagashima S., Zhou X., Ghosh S., Peng J. S., Hu Q., Zhou D. J., Yang Z. Q.. ( 2010;). Full genomic analysis of a porcine-bovine reassortant G4P[6] rotavirus strain R479 isolated from an infant in China. . J Med Virol 82:, 1094–1102. [CrossRef][PubMed]
    [Google Scholar]
  35. Wu H., Taniguchi K., Urasawa T., Urasawa S.. ( 1998;). Serological and genomic characterization of human rotaviruses detected in China. . J Med Virol 55:, 168–176. [CrossRef][PubMed]
    [Google Scholar]
  36. Yamamoto D., Kawaguchiya M., Ghosh S., Ichikawa M., Numazaki K., Kobayashi N.. ( 2011;). Detection and full genomic analysis of G6P[9] human rotavirus in Japan. . Virus Genes 43:, 215–223. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.043026-0
Loading
/content/journal/jgv/10.1099/vir.0.043026-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error