1887

Abstract

Sindbis virus (SINV) is an arthropod-borne alphavirus, which causes rash-arthritis, particularly in Finland. SINV is transmitted by mosquitoes in Finland but thus far no virus has been isolated from mosquitoes. In this study, we report the isolation of the first SINV strain from mosquitoes in Finland and its full-length protein-coding sequence. We furthermore describe the full-length coding sequence of six SINV strains previously isolated from humans in Finland and from a mosquito in Russia. The strain isolated from mosquitoes (Ilomantsi-2005M) was very closely related to all the other Northern European SINV strains. We found 9 aa positions, of which five in the nsP3 protein C terminus, to be distinctive signatures for the Northern European strains that may be associated with vector or host species adaptation. Phylogenetic analyses further indicate that SINV has a local circulation in endemic regions in Northern Europe and no novel strains are frequently being introduced.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.042853-0
2012-09-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/93/9/1984.html?itemId=/content/journal/jgv/10.1099/vir.0.042853-0&mimeType=html&fmt=ahah

References

  1. Bandelt H. J., Forster P., Röhl A.. ( 1999;). Median-joining networks for inferring intraspecific phylogenies. . Mol Biol Evol 16:, 37–48. [CrossRef][PubMed]
    [Google Scholar]
  2. Brummer-Korvenkontio M., Vapalahti O., Kuusisto P., Saikku P., Manni T., Koskela P., Nygren T., Brummer-Korvenkontio H., Vaheri A.. ( 2002;). Epidemiology of Sindbis virus infections in Finland 1981-96: possible factors explaining a peculiar disease pattern. . Epidemiol Infect 129:, 335–345. [CrossRef][PubMed]
    [Google Scholar]
  3. I., Fata-Hartley C., Sawicki S. G., Sawicki D. L.. ( 2003;). Functional analysis of nsP3 phosphoprotein mutants of Sindbis virus. . J Virol 77:, 13106–13116. [CrossRef][PubMed]
    [Google Scholar]
  4. Edgar R. C.. ( 2004;). muscle: a multiple sequence alignment method with reduced time and space complexity. . BMC Bioinformatics 5:, 113. [CrossRef][PubMed]
    [Google Scholar]
  5. Folmer O., Black M., Hoeh W., Lutz R., Vrijenhoek R.. ( 1994;). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. . Mol Mar Biol Biotechnol 3:, 294–299.[PubMed]
    [Google Scholar]
  6. Francy D. B., Jaenson T. G., Lundström J. O., Schildt E. B., Espmark A., Henriksson B., Niklasson B.. ( 1989;). Ecologic studies of mosquitoes and birds as hosts of Ockelbo virus in Sweden and isolation of Inkoo and Batai viruses from mosquitoes. . Am J Trop Med Hyg 41:, 355–363.[PubMed]
    [Google Scholar]
  7. Gould E. A., Coutard B., Malet H., Morin B., Jamal S., Weaver S., Gorbalenya A., Moureau G., Baronti C.. & other authors ( 2010;). Understanding the alphaviruses: recent research on important emerging pathogens and progress towards their control. . Antiviral Res 87:, 111–124. [CrossRef][PubMed]
    [Google Scholar]
  8. Hall T. A.. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  9. Hubálek Z.. ( 2008;). Mosquito-borne viruses in Europe. . Parasitol Res 103: (Suppl. 1), S29–S43. [CrossRef][PubMed]
    [Google Scholar]
  10. Jöst H., Bialonski A., Storch V., Günther S., Becker N., Schmidt-Chanasit J.. ( 2010;). Isolation and phylogenetic analysis of Sindbis viruses from mosquitoes in Germany. . J Clin Microbiol 48:, 1900–1903. [CrossRef][PubMed]
    [Google Scholar]
  11. Jöst H., Bürck-Kammerer S., Hütter G., Lattwein E., Lederer S., Litzba N., Bock-Hensley O., Emmerich P., Günther S.. & other authors ( 2011;). Medical importance of Sindbis virus in south-west Germany. . J Clin Virol 52:, 278–279. [CrossRef][PubMed]
    [Google Scholar]
  12. Korber B.. ( 2000;). HIV signature and sequence variation analysis. . In Computational Analysis of HIV Molecular Sequences, pp. 55–72. Edited by Rodrigo A., Learn G... Dordrecht, Netherlands:: Kluwer Academic Publishers;.
    [Google Scholar]
  13. Kurkela S., Manni T., Vaheri A., Vapalahti O.. ( 2004;). Causative agent of Pogosta disease isolated from blood and skin lesions. . Emerg Infect Dis 10:, 889–894. [CrossRef][PubMed]
    [Google Scholar]
  14. Kurkela S., Manni T., Myllynen J., Vaheri A., Vapalahti O.. ( 2005;). Clinical and laboratory manifestations of Sindbis virus infection: prospective study, Finland, 2002-2003. . J Infect Dis 191:, 1820–1829. [CrossRef][PubMed]
    [Google Scholar]
  15. Kurkela S., Helve T., Vaheri A., Vapalahti O.. ( 2008a;). Arthritis and arthralgia three years after Sindbis virus infection: clinical follow-up of a cohort of 49 patients. . Scand J Infect Dis 40:, 167–173. [CrossRef][PubMed]
    [Google Scholar]
  16. Kurkela S., Rätti O., Huhtamo E., Uzcátegui N. Y., Nuorti J. P., Laakkonen J., Manni T., Helle P., Vaheri A., Vapalahti O.. ( 2008b;). Sindbis virus infection in resident birds, migratory birds, and humans, Finland. . Emerg Infect Dis 14:, 41–47. [CrossRef][PubMed]
    [Google Scholar]
  17. L’vov D. K., Skvortsova T. M., GromashevskiIˇ V. L., Berezina L. K., Iakovlev V. I.. ( 1985;). [Isolation of the causative agent of Karelian fever from Aedes sp. mosquitoes]. . Vopr Virusol 30:, 311–313 (in Russian).[PubMed]
    [Google Scholar]
  18. Laine M., Luukkainen R., Jalava J., Ilonen J., Kuusistö P., Toivanen A.. ( 2000;). Prolonged arthritis associated with Sindbis-related (Pogosta) virus infection. . Rheumatology (Oxford) 39:, 1272–1274. [CrossRef][PubMed]
    [Google Scholar]
  19. Lastarza M. W., Grakoui A., Rice C. M.. ( 1994;). Deletion and duplication mutations in the C-terminal nonconserved region of Sindbis virus nsP3: effects on phosphorylation and on virus replication in vertebrate and invertebrate cells. . Virology 202:, 224–232. [CrossRef][PubMed]
    [Google Scholar]
  20. Li G. P., La Starza M. W., Hardy W. R., Strauss J. H., Rice C. M.. ( 1990;). Phosphorylation of Sindbis virus nsP3 in vivo and in vitro. . Virology 179:, 416–427. [CrossRef][PubMed]
    [Google Scholar]
  21. Lundström J. O., Pfeffer M.. ( 2010;). Phylogeographic structure and evolutionary history of Sindbis virus. . Vector Borne Zoonotic Dis 10:, 889–907. [CrossRef][PubMed]
    [Google Scholar]
  22. Lunt D. H., Zhang D. X., Szymura J. M., Hewitt G. M.. ( 1996;). The insect cytochrome oxidase I gene: evolutionary patterns and conserved primers for phylogenetic studies. . Insect Mol Biol 5:, 153–165. [CrossRef][PubMed]
    [Google Scholar]
  23. Malet H., Coutard B., Jamal S., Dutartre H., Papageorgiou N., Neuvonen M., Ahola T., Forrester N., Gould E. A.. & other authors ( 2009;). The crystal structures of Chikungunya and Venezuelan equine encephalitis virus nsP3 macro domains define a conserved adenosine binding pocket. . J Virol 83:, 6534–6545. [CrossRef][PubMed]
    [Google Scholar]
  24. Meissner J. D., Huang C. Y., Pfeffer M., Kinney R. M.. ( 1999;). Sequencing of prototype viruses in the Venezuelan equine encephalitis antigenic complex. . Virus Res 64:, 43–59. [CrossRef][PubMed]
    [Google Scholar]
  25. Niklasson B., Espmark A.. ( 1986;). Ockelbo disease: arthralgia 3-4 years after infection with a Sindbis virus related agent. . Lancet 327:, 1039–1040. [CrossRef][PubMed]
    [Google Scholar]
  26. Niklasson B., Espmark A., LeDuc J. W., Gargan T. P., Ennis W. A., Tesh R. B., Main A. J. Jr. ( 1984;). Association of a Sindbis-like virus with Ockelbo disease in Sweden. . Am J Trop Med Hyg 33:, 1212–1217.[PubMed]
    [Google Scholar]
  27. Oberste M. S., Parker M. D., Smith J. F.. ( 1996;). Complete sequence of Venezuelan equine encephalitis virus subtype IE reveals conserved and hypervariable domains within the C terminus of nsP3. . Virology 219:, 314–320. [CrossRef][PubMed]
    [Google Scholar]
  28. Powers A. M., Brault A. C., Shirako Y., Strauss E. G., Kang W., Strauss J. H., Weaver S. C.. ( 2001;). Evolutionary relationships and systematics of the alphaviruses. . J Virol 75:, 10118–10131. [CrossRef][PubMed]
    [Google Scholar]
  29. Powers A., Huang H., Roehrig J., Strauss E., Weaver S. C.. ( 2012;). Family - Togaviridae. . In Virus Taxonomy, pp. 1103–1110. Edited by King A. M. Q., Lefkowitz E., Adams M. J., Carstens E. B... San Diego:: Elsevier;.
    [Google Scholar]
  30. Reinert J. F.. ( 2000;). New classification for the composite genus Aedes (Diptera: Culicidae: Aedini), elevation of subgenus Ochlerotatus to generic rank, reclassification of the other subgenera, and notes on certain subgenera and species. . J Am Mosq Control Assoc 16:, 175–188.[PubMed]
    [Google Scholar]
  31. Sallum M. A. M., Schultz T. R., Foster P. G., Aronstein K., Wirtz R. A., Wilkerson R. C.. ( 2002;). Phylogeny of anophelinae (Diptera: Culicidae) based on nuclear ribosomal and mitochondrial DNA sequences. . Syst Entomol 27:, 361–382. [CrossRef]
    [Google Scholar]
  32. Sane J., Guedes S., Kurkela S., Lyytikäinen O., Vapalahti O.. ( 2010;). Epidemiological analysis of mosquito-borne Pogosta disease in Finland, 2009. . Euro Surveill 15:, 19462.[PubMed]
    [Google Scholar]
  33. Sane J., Guedes S., Ollgren J., Kurkela S., Klemets P., Vapalahti O., Kela E., Lyytikäinen O., Nuorti J. P.. ( 2011;). Epidemic Sindbis virus infection in Finland: a population-based case-control study of risk factors. . J Infect Dis 204:, 459–466. [CrossRef][PubMed]
    [Google Scholar]
  34. Skogh M., Espmark A.. ( 1982;). Ockelbo disease: epidemic arthritis-exanthema syndrome in Sweden caused by Sindbis-virus like agent. . Lancet 319:, 795–796. [CrossRef][PubMed]
    [Google Scholar]
  35. Stamatakis A., Hoover P., Rougemont J.. ( 2008;). A rapid bootstrap algorithm for the RAxML Web servers. . Syst Biol 57:, 758–771. [CrossRef][PubMed]
    [Google Scholar]
  36. Strauss J. H., Strauss E. G.. ( 1994;). The alphaviruses: gene expression, replication, and evolution. . Microbiol Rev 58:, 491–562.[PubMed]
    [Google Scholar]
  37. Strauss E. G., Rice C. M., Strauss J. H.. ( 1984;). Complete nucleotide sequence of the genomic RNA of Sindbis virus. . Virology 133:, 92–110. [CrossRef][PubMed]
    [Google Scholar]
  38. Turunen M., Kuusisto P., Uggeldahl P. E., Toivanen A.. ( 1998;). Pogosta disease: clinical observations during an outbreak in the province of North Karelia, Finland. . Br J Rheumatol 37:, 1177–1180. [CrossRef][PubMed]
    [Google Scholar]
  39. Utrio P.. ( 1977;). A checklist of Finnish mosquitoes (Diptera, Culicidae), including Aedes Annulipens, new to Finland. . Notulae Entomologicae 57:, 130–132.
    [Google Scholar]
  40. Vihinen H., Ahola T., Tuittila M., Merits A., Kääriäinen L.. ( 2001;). Elimination of phosphorylation sites of Semliki Forest virus replicase protein nsP3. . J Biol Chem 276:, 5745–5752. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.042853-0
Loading
/content/journal/jgv/10.1099/vir.0.042853-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error