1887

Abstract

Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potential pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of double-infection using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and Madin–Darby canine kidney cells were detected by confocal microscopy and flow cytometry.

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2012-08-01
2019-10-18
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References

  1. Chen L. M. , Davis C. T. , Zhou H. , Cox N. J. , Donis R. O. . ( 2008; ). Genetic compatibility and virulence of reassortants derived from contemporary avian H5N1 and human H3N2 influenza A viruses. . PLoS Pathog 4:, e1000072. [CrossRef] [PubMed]
    [Google Scholar]
  2. Garten R. J. , Davis C. T. , Russell C. A. , Shu B. , Lindstrom S. , Balish A. , Sessions W. M. , Xu X. , Skepner E. . & other authors ( 2009; ). Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans. . Science 325:, 197–201. [CrossRef] [PubMed]
    [Google Scholar]
  3. Greenbaum B. D. , Li O. T. , Poon L. L. , Levine A. J. , Rabadan R. . ( 2012; ). Viral reassortment as an information exchange between viral segments. . Proc Natl Acad Sci U S A 109:, 3341–3346. [CrossRef] [PubMed]
    [Google Scholar]
  4. Huang I. C. , Li W. , Sui J. , Marasco W. , Choe H. , Farzan M. . ( 2008; ). Influenza A virus neuraminidase limits viral superinfection. . J Virol 82:, 4834–4843. [CrossRef] [PubMed]
    [Google Scholar]
  5. Hutchinson E. C. , von Kirchbach J. C. , Gog J. R. , Digard P. . ( 2010; ). Genome packaging in influenza A virus. . J Gen Virol 91:, 313–328. [CrossRef] [PubMed]
    [Google Scholar]
  6. Ito T. , Couceiro J. N. , Kelm S. , Baum L. G. , Krauss S. , Castrucci M. R. , Donatelli I. , Kida H. , Paulson J. C. . & other authors ( 1998; ). Molecular basis for the generation in pigs of influenza A viruses with pandemic potential. . J Virol 72:, 7367–7373.[PubMed]
    [Google Scholar]
  7. Jackson S. , Van Hoeven N. , Chen L. M. , Maines T. R. , Cox N. J. , Katz J. M. , Donis R. O. . ( 2009; ). Reassortment between avian H5N1 and human H3N2 influenza viruses in ferrets: a public health risk assessment. . J Virol 83:, 8131–8140. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kida H. , Ito T. , Yasuda J. , Shimizu Y. , Itakura C. , Shortridge K. F. , Kawaoka Y. , Webster R. G. . ( 1994; ). Potential for transmission of avian influenza viruses to pigs. . J Gen Virol 75:, 2183–2188. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kimble J. B. , Sorrell E. , Shao H. , Martin P. L. , Perez D. R. . ( 2011; ). Compatibility of H9N2 avian influenza surface genes and 2009 pandemic H1N1 internal genes for transmission in the ferret model. . Proc Natl Acad Sci U S A 108:, 12084–12088. [CrossRef] [PubMed]
    [Google Scholar]
  10. Li C. , Hatta M. , Nidom C. A. , Muramoto Y. , Watanabe S. , Neumann G. , Kawaoka Y. . ( 2010; ). Reassortment between avian H5N1 and human H3N2 influenza viruses creates hybrid viruses with substantial virulence. . Proc Natl Acad Sci U S A 107:, 4687–4692. [CrossRef] [PubMed]
    [Google Scholar]
  11. Lubeck M. D. , Palese P. , Schulman J. L. . ( 1979; ). Nonrandom association of parental genes in influenza A virus recombinants. . Virology 95:, 269–274. [CrossRef] [PubMed]
    [Google Scholar]
  12. Ma W. , Lager K. M. , Vincent A. L. , Janke B. H. , Gramer M. R. , Richt J. A. . ( 2009; ). The role of swine in the generation of novel influenza viruses. . Zoonoses Public Health 56:, 326–337. [CrossRef] [PubMed]
    [Google Scholar]
  13. Novel Swine-Origin Influenza A (H1N1) Virus Investigation Team ( 2009; ). Emergence of a novel swine-origin influenza A (H1N1) virus in humans. . N Engl J Med 360:, 2605–2615. [CrossRef] [PubMed]
    [Google Scholar]
  14. Rabadan R. , Levine A. J. , Krasnitz M. . ( 2008; ). Non-random reassortment in human influenza A viruses. . Influenza Other Respir Viruses 2:, 9–22. [CrossRef] [PubMed]
    [Google Scholar]
  15. Rimmelzwaan G. F. , Nieuwkoop N. J. , de Mutsert G. , Boon A. C. , Kuiken T. , Fouchier R. A. , Osterhaus A. D. . ( 2007; ). Attachment of infectious influenza A viruses of various subtypes to live mammalian and avian cells as measured by flow cytometry. . Virus Res 129:, 175–181. [CrossRef] [PubMed]
    [Google Scholar]
  16. Rimmelzwaan G. F. , Verburgh R. J. , Nieuwkoop N. J. , Bestebroer T. M. , Fouchier R. A. , Osterhaus A. D. . ( 2011; ). Use of GFP-expressing influenza viruses for the detection of influenza virus A/H5N1 neutralizing antibodies. . Vaccine 29:, 3424–3430. [CrossRef] [PubMed]
    [Google Scholar]
  17. Schrauwen E. J. , Herfst S. , Chutinimitkul S. , Bestebroer T. M. , Rimmelzwaan G. F. , Osterhaus A. D. , Kuiken T. , Fouchier R. A. . ( 2011; ). Possible increased pathogenicity of pandemic (H1N1) 2009 influenza virus upon reassortment. . Emerg Infect Dis 17:, 200–208.[PubMed] [CrossRef]
    [Google Scholar]
  18. Shaner N. C. , Steinbach P. A. , Tsien R. Y. . ( 2005; ). A guide to choosing fluorescent proteins. . Nat Methods 2:, 905–909. [CrossRef] [PubMed]
    [Google Scholar]
  19. Shinya K. , Ebina M. , Yamada S. , Ono M. , Kasai N. , Kawaoka Y. . ( 2006; ). Avian flu: influenza virus receptors in the human airway. . Nature 440:, 435–436. [CrossRef] [PubMed]
    [Google Scholar]
  20. Sun Y. , Qin K. , Wang J. , Pu J. , Tang Q. , Hu Y. , Bi Y. , Zhao X. , Yang H. . & other authors ( 2011; ). High genetic compatibility and increased pathogenicity of reassortants derived from avian H9N2 and pandemic H1N1/2009 influenza viruses. . Proc Natl Acad Sci U S A 108:, 4164–4169. [CrossRef] [PubMed]
    [Google Scholar]
  21. Suzuki Y. , Ito T. , Suzuki T. , Holland R. E. Jr , Chambers T. M. , Kiso M. , Ishida H. , Kawaoka Y. . ( 2000; ). Sialic acid species as a determinant of the host range of influenza A viruses. . J Virol 74:, 11825–11831. [CrossRef] [PubMed]
    [Google Scholar]
  22. Ungchusak K. , Auewarakul P. , Dowell S. F. , Kitphati R. , Auwanit W. , Puthavathana P. , Uiprasertkul M. , Boonnak K. , Pittayawonganon C. . & other authors ( 2005; ). Probable person-to-person transmission of avian influenza A (H5N1). . N Engl J Med 352:, 333–340. [CrossRef] [PubMed]
    [Google Scholar]
  23. van Riel D. , den Bakker M. A. , Leijten L. M. , Chutinimitkul S. , Munster V. J. , de Wit E. , Rimmelzwaan G. F. , Fouchier R. A. , Osterhaus A. D. , Kuiken T. . ( 2010; ). Seasonal and pandemic human influenza viruses attach better to human upper respiratory tract epithelium than avian influenza viruses. . Am J Pathol 176:, 1614–1618. [CrossRef] [PubMed]
    [Google Scholar]
  24. Wang H. , Feng Z. , Shu Y. , Yu H. , Zhou L. , Zu R. , Huai Y. , Dong J. , Bao C. . & other authors ( 2008; ). Probable limited person-to-person transmission of highly pathogenic avian influenza A (H5N1) virus in China. . Lancet 371:, 1427–1434. [CrossRef] [PubMed]
    [Google Scholar]
  25. WHO Site accessed April 13 ( 2012; ). Cumulative Number of Confirmed Human Cases of Avian Influenza A/(H5N1) Reported to World Health Organisation. . Available at http://www.who.int/influenza/human_animal_interface/EN_GIP_20120412CumulativeNumberH5N1cases.pdf
  26. Writing Committee of the Second World Health Organization Consultation on Clinical Aspects of Human 195 Infection with Avian Influenza, A Virus ( 2008; ). Update on avian influenza A (H5N1) virus infection in humans. . N Engl J Med 358:, 261–273. [CrossRef] [PubMed]
    [Google Scholar]
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