Two suppressors of RNA silencing encoded by cereal-infecting members of the family Free

Abstract

Several members of the family are important pathogens of cultivated plant species of the family Gramineae. In this study, we explored RNA-silencing suppressors (RSSs) encoded by two cereal-infecting luteoviruses: barley yellow dwarf virus and wheat yellow dwarf virus (BYDV and WYDV, respectively). The P0 protein of WYDV-GPV (P0) and the P6 protein of BYDV-GAV (P6) displayed RSS activities when expressed in agro-infiltrated leaves of , by their local ability to inhibit post-transcriptional gene silencing of GFP. Analysis of GFP, mRNA and GFP-specific small interfering RNA indicated that both P0 and P6 are suppressors of silencing that can restrain not only local but also systemic gene silencing. This is the first report of RSS activity of the P6 protein in a member of the genus .

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2012-08-01
2024-03-29
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