1887

Abstract

Several members of the family are important pathogens of cultivated plant species of the family Gramineae. In this study, we explored RNA-silencing suppressors (RSSs) encoded by two cereal-infecting luteoviruses: barley yellow dwarf virus and wheat yellow dwarf virus (BYDV and WYDV, respectively). The P0 protein of WYDV-GPV (P0) and the P6 protein of BYDV-GAV (P6) displayed RSS activities when expressed in agro-infiltrated leaves of , by their local ability to inhibit post-transcriptional gene silencing of GFP. Analysis of GFP, mRNA and GFP-specific small interfering RNA indicated that both P0 and P6 are suppressors of silencing that can restrain not only local but also systemic gene silencing. This is the first report of RSS activity of the P6 protein in a member of the genus .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.042135-0
2012-08-01
2020-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/93/8/1825.html?itemId=/content/journal/jgv/10.1099/vir.0.042135-0&mimeType=html&fmt=ahah

References

  1. Alvarado V., Scholthof H. B.. ( 2009;). Plant responses against invasive nucleic acids: RNA silencing and its suppression by plant viral pathogens. . Semin Cell Dev Biol 20:, 1032–1040. [CrossRef][PubMed]
    [Google Scholar]
  2. Brigneti G., Voinnet O., Li W.-X., Ji L.-H., Ding S.-W., Baulcombe D. C.. ( 1998;). Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. . EMBO J 17:, 6739–6746. [CrossRef][PubMed]
    [Google Scholar]
  3. Burgyán J., Havelda Z.. ( 2011;). Viral suppressors of RNA silencing. . Trends Plant Sci 16:, 265–272. [CrossRef][PubMed]
    [Google Scholar]
  4. Csorba T., Pantaleo V., Burgyán J.. ( 2009;). RNA silencing: an antiviral mechanism. . Adv Virus Res 75:, 35–71. [CrossRef][PubMed]
    [Google Scholar]
  5. Goodin M. M., Dietzgen R. G., Schichnes D., Ruzin S., Jackson A. O.. ( 2002;). pGD vectors: versatile tools for the expression of green and red fluorescent protein fusions in agroinfiltrated plant leaves. . Plant J 31:, 375–383. [CrossRef][PubMed]
    [Google Scholar]
  6. Goto K., Kobori T., Kosaka Y., Natsuaki T., Masuta C.. ( 2007;). Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities. . Plant Cell Physiol 48:, 1050–1060. [CrossRef][PubMed]
    [Google Scholar]
  7. Gray S., Gildow F. E.. ( 2003;). Luteovirus–aphid interactions. . Annu Rev Phytopathol 41:, 539–566. [CrossRef][PubMed]
    [Google Scholar]
  8. Hamilton A. J., Baulcombe D. C.. ( 1999;). A species of small antisense RNA in posttranscriptional gene silencing in plants. . Science 286:, 950–952. [CrossRef][PubMed]
    [Google Scholar]
  9. Hamilton C. M., Frary A., Lewis C., Tanksley S. D.. ( 1996;). Stable transfer of intact high molecular weight DNA into plant chromosomes. . Proc Natl Acad Sci U S A 93:, 9975–9979. [CrossRef][PubMed]
    [Google Scholar]
  10. Hamilton A., Voinnet O., Chappell L., Baulcombe D. C.. ( 2002;). Two classes of short interfering RNA in RNA silencing. . EMBO J 21:, 4671–4679. [CrossRef][PubMed]
    [Google Scholar]
  11. Han Y. H., Xiang H. Y., Wang Q., Li Y. Y., Wu W. Q., Han C. G., Li D. W., Yu J. L.. ( 2010;). Ring structure amino acids affect the suppressor activity of melon aphid-borne yellows virus P0 protein. . Virology 406:, 21–27. [CrossRef][PubMed]
    [Google Scholar]
  12. Jin Z. B., Wang X. F., Chang S. J., Zhou G. H.. ( 2004;). The complete nucleotide sequence and its organization of the genome of barley yellow dwarf virus-GAV. . Sci China C Life Sci 47:, 175–182. [CrossRef][PubMed]
    [Google Scholar]
  13. Johansen L. K., Carrington J. C.. ( 2001;). Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system. . Plant Physiol 126:, 930–938. [CrossRef][PubMed]
    [Google Scholar]
  14. King A. M. Q., Adams M. J., Carstens E. B., Lefkowitz E. J.. ( 2011;). Virus Taxonomy: Classification and Nomenclature of Viruses. Ninth Report of the International Committee on Taxonomy of Viruses. Amsterdam:: Elsevier Academic Press;.
    [Google Scholar]
  15. Kozlowska-Makulska A., Guilley H., Szyndel M. S., Beuve M., Lemaire O., Herrbach E., Bouzoubaa S.. ( 2010;). P0 proteins of European beet-infecting poleroviruses display variable RNA silencing suppression activity. . J Gen Virol 91:, 1082–1091. [CrossRef][PubMed]
    [Google Scholar]
  16. Kvarnheden A.. ( 2011;). Viruses of field crops – an overview. . In NJF Seminar 446: Risk Assessment/Risk Management, Forecasting Pests and Diseases of Field Crops in a Changing Climate – Control Strategies for Pests, Diseases And Weeds, pp. 53–58. Kristianstad, Sweden:: Nordic Association of Agricultural Scientists;. http://www.njf.nu/filebank/files/20120109$173226$fil$TWC10udR6xv5gUrzM337.pdf
    [Google Scholar]
  17. Liu S., Bugos R. C., Dharmasiri N., Su W. W.. ( 2001;). Green fluorescent protein as a secretory reporter and a tool for process optimization in transgenic plant cell cultures. . J Biotechnol 87:, 1–16. [CrossRef][PubMed]
    [Google Scholar]
  18. Liu F., Wang X. F., Liu Y., Xie J. J., Gray S. M., Zhou G. H., Gao B. D.. ( 2007a;). A Chinese isolate of barley yellow dwarf virus-PAV represents a third distinct species within the PAV serotype. . Arch Virol 152:, 1365–1373. [CrossRef][PubMed]
    [Google Scholar]
  19. Liu Y., Sun B., Wang X. F., Zheng C. L., Zhou G. H.. ( 2007b;). Three digoxigenin-labeled cDNA probes for specific detection of the natural population of barley yellow dwarf viruses in China by dot-blot hybridization. . J Virol Methods 145:, 22–29. [CrossRef][PubMed]
    [Google Scholar]
  20. Mangwende T., Wang M.-L., Borth W., Hu J., Moore P. H., Mirkov T. E., Albert H. H.. ( 2009;). The P0 gene of Sugarcane yellow leaf virus encodes an RNA silencing suppressor with unique activities. . Virology 384:, 38–50. [CrossRef][PubMed]
    [Google Scholar]
  21. Pfeffer S., Dunoyer P., Heim F., Richards K. E., Jonard G., Ziegler-Graff V.. ( 2002;). P0 of beet Western yellows virus is a suppressor of posttranscriptional gene silencing. . J Virol 76:, 6815–6824. [CrossRef][PubMed]
    [Google Scholar]
  22. Rochow W. F.. ( 1969;). Biological properties of four isolates of barley yellow dwarf virus. . Phytopathology 59:, 1580–1589.[PubMed]
    [Google Scholar]
  23. Rochow W. F., Muller I.. ( 1971;). A fifth variant of barley yellow dwarf virus in New York. . Plant Dis 55:, 874–879.
    [Google Scholar]
  24. Ruiz M. T., Voinnet O., Baulcombe D. C.. ( 1998;). Initiation and maintenance of virus-induced gene silencing. . Plant Cell 10:, 937–946. [CrossRef][PubMed]
    [Google Scholar]
  25. Senda M., Masuta C., Ohnishi S., Goto K., Kasai A., Sano T., Hong J.-S., MacFarlane S.. ( 2004;). Patterning of virus-infected Glycine max seed coat is associated with suppression of endogenous silencing of chalcone synthase genes. . Plant Cell 16:, 807–818. [CrossRef][PubMed]
    [Google Scholar]
  26. van Engelen F. A., Molthoff J. W., Conner A. J., Nap J. P., Pereira A., Stiekema W. J.. ( 1995;). pBINPLUS: an improved plant transformation vector based on pBIN19. . Transgenic Res 4:, 288–290. [CrossRef][PubMed]
    [Google Scholar]
  27. Wu B. L., Blanchard-Letort A., Liu Y., Zhou G. H., Wang X. F., Elena S. F.. ( 2011;). Dynamics of molecular evolution and phylogeography of Barley yellow dwarf virus-PAV. . PLoS ONE 6:, e16896. [CrossRef][PubMed]
    [Google Scholar]
  28. Wydro M., Kozubek E., Lehmann P.. ( 2006;). Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana. . Acta Biochim Pol 53:, 289–298.[PubMed]
    [Google Scholar]
  29. Xiong R. Y., Wu J. X., Zhou Y. J., Zhou X. P.. ( 2009;). Characterization and subcellular localization of an RNA silencing suppressor encoded by Rice stripe tenuivirus. . Virology 387:, 29–40. [CrossRef][PubMed]
    [Google Scholar]
  30. Zhang W. W., Cheng Z. M., Xu L., Wu M. S., Waterhouse P., Zhou G. H., Li S. F.. ( 2009;). The complete nucleotide sequence of the barley yellow dwarf GPV isolate from China shows that it is a new member of the genus Polerovirus. . Arch Virol 154:, 1125–1128. [CrossRef][PubMed]
    [Google Scholar]
  31. Zhou G. H., Zhang S. X., Qian Y. T.. ( 1987;). Identification and applications of four strains of wheat yellow dwarf virus. . Sci Agric 20:, 7–12.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.042135-0
Loading
/content/journal/jgv/10.1099/vir.0.042135-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error