RT Journal Article SR Electronic(1) A1 Ma, Wenjun A1 Liu, Qinfang A1 Bawa, Bhupinder A1 Qiao, Chuanling A1 Qi, Wenbao A1 Shen, Huigang A1 Chen, Ying A1 Ma, Jingqun A1 Li, Xi A1 Webby, Richard J. A1 García-Sastre, Adolfo A1 Richt, Jürgen A.YR 2012 T1 The neuraminidase and matrix genes of the 2009 pandemic influenza H1N1 virus cooperate functionally to facilitate efficient replication and transmissibility in pigs JF Journal of General Virology, VO 93 IS 6 SP 1261 OP 1268 DO https://doi.org/10.1099/vir.0.040535-0 PB Microbiology Society, SN 1465-2099, AB The 2009 pandemic H1N1 virus (pH1N1) contains neuraminidase (NA) and matrix (M) genes from Eurasian avian-like swine influenza viruses (SIVs), with the remaining six genes from North American triple-reassortant SIVs. To characterize the role of the pH1N1 NA and M genes in pathogenesis and transmission, their impact was evaluated in the background of an H1N1 triple-reassortant (tr1930) SIV in which the HA (H3) and NA (N2) of influenza A/swine/Texas/4199-2/98 virus were replaced with those from the classical H1N1 A/swine/Iowa/15/30 (1930) virus. The laboratory-adapted 1930 virus did not shed nor transmit in pigs, but tr1930 was able to shed in infected pigs. The NA, M or both genes of the tr1930 virus were then substituted by those of pH1N1. The resulting virus with both NA and M from pH1N1 grew to significantly higher titre in cell cultures than the viruses with single NA or M from pH1N1. In a pig model, only the virus containing both NA and M from pH1N1 was transmitted to and infected sentinels, whereas the viruses with single NA or M from pH1N1 did not. These results demonstrate that the right combination of NA and M genes is critical for the replication and transmissibility of influenza viruses in pigs., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.040535-0