Bats constitute a reservoir of zoonotic infections and some bat paramyxoviruses are capable of cross-species transmission, often with fatal consequences. Determining the level of viral diversity in reservoir populations is fundamental to understanding and predicting viral emergence. This is particularly relevant for RNA viruses where the adaptive mutations required for cross-species transmission can be present in the reservoir host. We report the use of non-invasively collected, pooled, neat urine samples as a robust sample type for investigating paramyxoviruses in bat populations. Using consensus PCR assays we have detected a high incidence and genetic diversity of novel paramyxoviruses in an urban fruit bat population over a short period of time. This may suggest a similarly unique relationship between bats and the members of the family Paramyxoviridae as proposed for some other viral families. Additionally, the high rate of bat–human contact at the study site calls for the zoonotic potential of the detected viruses to be investigated further.
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ChantK.,
ChanR.,
SmithM.,
DwyerD. E.,
KirklandP. D.The NSW Expert Group1998; Probable human infection with a newly described virus in the family Paramyxoviridae. Emerg Infect Dis 4:273–275 [View Article][PubMed]
ChuaK. B.2003; A novel approach for collecting samples from fruit bats for isolation of infectious agents. Microbes Infect 5:487–490 [View Article][PubMed]
DelmasO.,
HolmesE. C.,
TalbiC.,
LarrousF.,
DacheuxL.,
BouchierC.,
BourhyH.2008; Genomic diversity and evolution of the lyssaviruses. PLoS ONE 3:e2057 [View Article][PubMed]
GouilhM. A.,
PuechmailleS. J.,
GonzalezJ. P.,
TeelingE.,
KittayapongP.,
ManuguerraJ. C.2011; SARS-Coronavirus ancestor’s foot-prints in South-East Asian bat colonies and the refuge theory. Infect Genet Evol 11:1690–1702 [View Article]
HaymanD. T.,
Suu-IreR.,
BreedA. C.,
McEachernJ. A.,
WangL.,
WoodJ. L.,
CunninghamA. A.2008; Evidence of henipavirus infection in West African fruit bats. PLoS ONE 3:e2739 [View Article][PubMed]
KaminsA. O.,
RestifO.,
Ntiamoa-BaiduY.,
Suu-IreR.,
HaymanD. T. S.,
CunninghamA. A.,
WoodJ. L. N.,
RowcliffeJ. M.2011; Uncovering the fruit bat bushmeat commodity chain and the true extent of fruit bat hunting in Ghana, West Africa. Biol Conserv 144:3000–3008[CrossRef]
LauS. K.,
WooP. C.,
WongB. H.,
WongA. Y.,
TsoiH. W.,
WangM.,
LeeP.,
XuH.,
PoonR. W.,
GuoR.2010; Identification and complete genome analysis of three novel paramyxoviruses, tuhoko virus 1, 2 and 3, in fruit bats from China. Virology 404:106–116 [View Article][PubMed]
MiddletonD. J.,
MorrissyC. J.,
van der HeideB. M.,
RussellG. M.,
BraunM. A.,
WestburyH. A.,
HalpinK.,
DanielsP. W.2007; Experimental nipah virus infection in pteropid bats (Pteropus poliocephalus). J Comp Pathol 136:266–272 [View Article][PubMed]
ThompsonJ. D.,
HigginsD. G.,
GibsonT. J.1994; clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]