RT Journal Article SR Electronic(1) A1 Muller, David A. A1 Landsberg, Michael J. A1 Bletchly, Cheryl A1 Rothnagel, Rosalba A1 Waddington, Lynne A1 Hankamer, Ben A1 Young, Paul R.YR 2012 T1 Structure of the dengue virus glycoprotein non-structural protein 1 by electron microscopy and single-particle analysis JF Journal of General Virology, VO 93 IS 4 SP 771 OP 779 DO https://doi.org/10.1099/vir.0.039321-0 PB Microbiology Society, SN 1465-2099, AB The flavivirus non-structural protein 1 (NS1) is a glycoprotein that is secreted as a soluble hexameric complex during the course of natural infection. Growing evidence indicates that this secreted form of NS1 (sNS1) plays a significant role in immune evasion and modulation during infection. Attempts to determine the crystal structure of NS1 have been unsuccessful to date and relatively little is known about the macromolecular organization of the sNS1 hexamer. Here, we have applied single-particle analysis to images of baculovirus-derived recombinant dengue 2 virus NS1 obtained by electron microscopy to determine its 3D structure to a resolution of 23 Å. This structure reveals a barrel-like organization of the three dimeric units that comprise the hexamer and provides further insights into the overall organization of oligomeric sNS1., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.039321-0