1887

Abstract

Infectious bursal disease virus (IBDV) causes an important disease in young chickens. Chicken heat-shock protein 90 (cHsp90) has been shown to be a functional component of the cellular receptor complex for IBDV infection. This study demonstrates the inhibitory effect of vector-expressed anti-α microRNA (miRNA) on IBDV infection. The reporter vectors pcHsp90α-EGFP and pcHsp90β-EGFP were constructed to facilitate effective miRNA selection. Two anti-α and one anti-β miRNA-expression vectors were constructed for a stable transfection study. Poly(A)-tailed RT-PCR detected sequence-specific miRNA transcription in transfected cells. Semiquantitative RT-PCR showed inhibition of transcription in transfected cells. A virus-titration assay showed that the anti-α miRNA, but not the anti-β miRNA, had inhibitory effects on IBDV infection. These results suggest that cHsp90α is a functional component of the cellular receptor complex for IBDV infection, and that anti-α miRNA could be used as an anti-IBDV reagent.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.039172-0
2012-04-01
2021-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/93/4/876.html?itemId=/content/journal/jgv/10.1099/vir.0.039172-0&mimeType=html&fmt=ahah

References

  1. Banerji U. 2009; Heat shock protein 90 as a drug target: some like it hot. Clin Cancer Res 15:9–14 [View Article][PubMed]
    [Google Scholar]
  2. Becht H. 1980; Infectious bursal disease virus. Curr Top Microbiol Immunol 90:107–121 [View Article][PubMed]
    [Google Scholar]
  3. Brown M. A., Zhu L., Schmidt C., Tucker P. W. 2007; Hsp90 – from signal transduction to cell transformation. Biochem Biophys Res Commun 363:241–246 [View Article][PubMed]
    [Google Scholar]
  4. Das R. M., Van Hateren N. J., Howell G. R., Farrell E. R., Bangs F. K., Porteous V. C., Manning E. M., McGrew M. J., Ohyama K., Sacco M. A. 2006; A robust system for RNA interference in the chicken using a modified microRNA operon. Dev Biol 294:554–563 [View Article][PubMed]
    [Google Scholar]
  5. Delgui L., Oña A., Gutiérrez S., Luque D., Navarro A., Castón J. R., Rodríguez J. F. 2009; The capsid protein of infectious bursal disease virus contains a functional α4β1 integrin ligand motif. Virology 386:360–372 [View Article][PubMed]
    [Google Scholar]
  6. Dobos P., Berthiaume L., Leong J. A., Kibenge F. S. B., Muller H., Nicholson S. L. 1995; Family Birnaviridae. In Virus Taxonomy: Classification and Nomenclature of Viruses. Sixth Report of the International Committee on Taxonomy of Viruses pp. 240–244 Edited by Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. New York: Springer;
    [Google Scholar]
  7. Fechner H., Pinkert S., Wang X., Sipo I., Suckau L., Kurreck J., Dörner A., Sollerbrant K., Zeichhardt H.other authors 2007; Coxsackievirus B3 and adenovirus infections of cardiac cells are efficiently inhibited by vector-mediated RNA interference targeting their common receptor. Gene Ther 14:960–971 [View Article][PubMed]
    [Google Scholar]
  8. Fire A., Xu S., Montgomery M. K., Kostas S. A., Driver S. E., Mello C. C. 1998; Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806–811 [View Article][PubMed]
    [Google Scholar]
  9. Gao Y. L., Liu W., Gao H. L., Qi X. L., Lin H., Wang X., Shen R. 2008; Effective inhibition of infectious bursal disease virus replication in vitro by DNA vector-based RNA interference. Antiviral Res 79:87–94 [View Article][PubMed]
    [Google Scholar]
  10. Goetz M. P., Toft D. O., Ames M. M., Erlichman C. 2003; The Hsp90 chaperone complex as a novel target for cancer therapy. Ann Oncol 14:1169–1176 [View Article][PubMed]
    [Google Scholar]
  11. Haywood A. M. 1994; Virus receptors: binding, adhesion strengthening and changes in viral structure. J Virol 68:1–5[PubMed]
    [Google Scholar]
  12. Lin T. W., Lo C. W., Lai S. Y., Fan R. J., Lo C. J., Chou Y. M., Thiruvengadam R., Wang A. H., Wang M. Y. 2007; Chicken heat shock protein 90 is a component of the putative cellular receptor complex of infectious bursal disease virus. J Virol 81:8730–8741 [View Article][PubMed]
    [Google Scholar]
  13. Lukert P. D., Saif Y. M. 1997; Infectious bursal disease. In Disease of Poultry pp. 721–737 Edited by Calnek B. W., Barnes H. J., Beard C. W., McDougald I. R., Saif Y. M. Ames, IA: Iowa State University Press;
    [Google Scholar]
  14. Luo J., Zhang H., Teng M., Fan J. M., You L. M., Xiao Z. J., Yi M. L., Zhi Y. B., Li X. W., Zhang G. P. 2010; Surface IgM on DT40 cells may be a component of the putative receptor complex responsible for the binding of infectious bursal disease virus. Avian Pathol 39:359–365 [View Article][PubMed]
    [Google Scholar]
  15. Martineau H. M., Pyrah I. 2007; Review of the application of RNA interference technology in the pharmaceutical industry. Toxicol Pathol 35:327–336 [View Article]
    [Google Scholar]
  16. Meister G., Tuschl T. 2004; Mechanisms of gene silencing by double-stranded RNA. Nature 431:343–349 [View Article][PubMed]
    [Google Scholar]
  17. Müller H., Islam M. R., Raue R. 2003; Research on infectious bursal disease – the past, the present and the future. Vet Microbiol 97:153–165 [View Article][PubMed]
    [Google Scholar]
  18. Nieper H., Müller H. 1996; Susceptibility of chicken lymphoid cells to infectious bursal disease virus does not correlate with the presence of specific binding sites. J Gen Virol 77:1229–1237 [View Article][PubMed]
    [Google Scholar]
  19. Sajjanar B. K., Mishra A., Sonawane A., Patel C. L., Saxena A., Dash B. B., Rai A., Raut A. A. 2011; Small interfering RNAs inhibit infectious bursal disease virus replication in Vero cells. Acta Virol 55:75–79 [View Article][PubMed]
    [Google Scholar]
  20. Setiyono A., Hayashi T. I, Yamaguchi T., Fukushi H., Hirai K. 2001; Detection of cell membrane proteins that interact with virulent infectious bursal disease virus. J Vet Med Sci 63:219–221 [View Article][PubMed]
    [Google Scholar]
  21. Sharma J. M., Kim I. J., Rautenschlein S., Yeh H. Y. 2000; Infectious bursal disease virus of chickens: pathogenesis and immunosuppression. Dev Comp Immunol 24:223–235 [View Article][PubMed]
    [Google Scholar]
  22. Van Den Berg T. P. 2000; Acute infectious bursal disease in poultry: a review. Avian Pathol 29:175–194 [View Article][PubMed]
    [Google Scholar]
  23. Wang Y., Sun H., Shen P., Zhang X., Xia X. 2009; Effective inhibition of infectious bursal disease virus replication by recombinant avian adeno-associated virus-delivered microRNAs. J Gen Virol 90:1417–1422 [View Article][PubMed]
    [Google Scholar]
  24. Wang Y., Sun H., Shen P., Zhang X., Xia X., Xia B. 2010; Effective inhibition of replication of infectious bursal disease virus by miRNAs delivered by vectors and targeting the VP2 gene. J Virol Methods 165:127–132 [View Article][PubMed]
    [Google Scholar]
  25. Zhou J., Rossi J. J. 2011; Progress in RNAi-based antiviral therapeutics. Methods Mol Biol 721:67–75 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.039172-0
Loading
/content/journal/jgv/10.1099/vir.0.039172-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error