1887

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Volume 93, Issue 5

Release of filamentous and spherical influenza A virus is not restricted by tetherin Free

Abstract

The cellular protein tetherin is thought to act as a ‘leash’ that anchors many enveloped viruses to the plasma membrane and prevents their release. We found that replication of multiple strains of influenza A virus was generally insensitive to alteration of tetherin levels, as assessed by output titre or scanning electron microscopy of cell-associated virions. This included human, swine, avian and equine isolates, strains that form filamentous or spherical particles and viruses that lack the M2 or NS1 proteins. Levels of cell-surface tetherin were not reduced by influenza infection, but tetherin and the viral haemagglutinin co-localized on the plasma membrane. However, tetherin could not be detected in filamentous virions, suggesting that influenza may possess a mechanism to exclude it from virions. Overall, if influenza does encode a specific antagonist of tetherin, it is not M2 or NS1 and we find no evidence for a role in host range specificity.

  • Received:
  • Accepted:
  • Published Online:
© 2012 SGM
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2012-05-01
2024-04-19
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Release of filamentous and spherical influenza A virus is not restricted by tetherin
J. Gen. Virol. 93, 963 (2012); https://doi.org/10.1099/vir.0.038778-0
/content/journal/jgv/10.1099/vir.0.038778-0
/content/journal/jgv/10.1099/vir.0.038778-0
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