1887

Abstract

This study is the first report of experimental infection and transmission of Menangle virus (MenPV) in pigs. Isolated in 1997 from piglets that were stillborn at a large commercial piggery in New South Wales, Australia, MenPV is a recently identified paramyxovirus of bat origin that causes severe reproductive disease in pigs and an influenza-like illness, with a rash, in humans. Although successfully eradicated from the infected piggery, the virus was only isolated from affected fetuses and stillborn piglets during the period of reproductive disease, and thus the mode of transmission between pigs was not established. To investigate the pathogenesis of MenPV, we undertook time-course studies in 6-week-old pigs following intranasal administration of a low-passage, non-plaque-purified isolate from the lung of an infected stillborn piglet. Viraemia was of short duration and low titre, as determined by real-time RT-PCR and virus isolation. Following an incubation period of 2–3 days, virus was shed in nasal and oral secretions, faeces and urine, typically for less than 1 week. Cessation of shedding correlated with the development of neutralizing antibodies in sera. Secondary lymphoid organs and intestine were identified, using quantitative real-time RT-PCR, as major sites of viral replication and dissemination, and this was confirmed by positive immunolabelling of viral antigen within various lymphoid tissues and intestinal epithelium. These data provide new insights into the pathogenesis of MenPV in weaned pigs, and will facilitate future control and eradication programmes should it ever re-emerge in the pig population.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.038448-0
2012-05-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/93/5/1007.html?itemId=/content/journal/jgv/10.1099/vir.0.038448-0&mimeType=html&fmt=ahah

References

  1. Bowden T. R. , Boyle D. B. . ( 2005; ). Completion of the full-length genome sequence of Menangle virus: characterisation of the polymerase gene and genomic 5′ trailer region. . Arch Virol 150:, 2125–2137. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bowden T. R. , Westenberg M. , Wang L. F. , Eaton B. T. , Boyle D. B. . ( 2001; ). Molecular characterization of Menangle virus, a novel paramyxovirus which infects pigs, fruit bats, and humans. . Virology 283:, 358–373. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bowden T. R. , Babiuk S. L. , Parkyn G. R. , Copps J. S. , Boyle D. B. . ( 2008; ). Capripoxvirus tissue tropism and shedding: a quantitative study in experimentally infected sheep and goats. . Virology 371:, 380–393. [CrossRef] [PubMed]
    [Google Scholar]
  4. Casteleyn C. , Breugelmans S. , Simoens P. , Van den Broeck W. . ( 2011; ). The tonsils revisited: review of the anatomical localization and histological characteristics of the tonsils of domestic and laboratory animals. . Clin Dev Immunol 2011:, 472460. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chant K. , Chan R. , Smith M. , Dwyer D. E. , Kirkland P. . The NSW Expert Group ( 1998; ). Probable human infection with a newly described virus in the family Paramyxoviridae . . Emerg Infect Dis 4:, 273–275. [CrossRef] [PubMed]
    [Google Scholar]
  6. de Swart R. L. , Ludlow M. , de Witte L. , Yanagi Y. , van Amerongen G. , McQuaid S. , Yüksel S. , Geijtenbeek T. B. H. , Duprex W. P. , Osterhaus A. D. M. E. . ( 2007; ). Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques. . PLoS Pathog 3:, e178. [CrossRef] [PubMed]
    [Google Scholar]
  7. Fleischer B. , Kreth H. W. . ( 1982; ). Mumps virus replication in human lymphoid cell lines and in peripheral blood lymphocytes: preference for T cells. . Infect Immun 35:, 25–31.[PubMed]
    [Google Scholar]
  8. Juozapaitis M. , Serva A. , Kucinskaite I. , Zvirbliene A. , Slibinskas R. , Staniulis J. , Sasnauskas K. , Shiell B. J. , Bowden T. R. , Michalski W. P. . ( 2007; ). Generation of Menangle virus nucleocapsid-like particles in yeast Saccharomyces cerevisiae . . J Biotechnol 130:, 441–447. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kirkland P. D. , Love R. J. , Philbey A. W. , Ross A. D. , Davis R. J. , Hart K. G. . ( 2001; ). Epidemiology and control of Menangle virus in pigs. . Aust Vet J 79:, 199–206. [CrossRef] [PubMed]
    [Google Scholar]
  10. Lin C. Y. , Chen W. P. , Chiang H. . ( 1990; ). Mumps associated with nephritis. . Child Nephrol Urol 10:, 68–71.[PubMed]
    [Google Scholar]
  11. Love R. J. , Philbey A. W. , Kirkland P. D. , Ross A. D. , Davis R. J. , Morrissey C. , Daniels P. W. . ( 2001; ). Reproductive disease and congenital malformations caused by Menangle virus in pigs. . Aust Vet J 79:, 192–198. [CrossRef] [PubMed]
    [Google Scholar]
  12. Middleton D. J. , Westbury H. A. , Morrissy C. J. , van der Heide B. M. , Russell G. M. , Braun M. A. , Hyatt A. D. . ( 2002; ). Experimental Nipah virus infection in pigs and cats. . J Comp Pathol 126:, 124–136. [CrossRef] [PubMed]
    [Google Scholar]
  13. Petraityte R. , Tamosiunas P. L. , Juozapaitis M. , Zvirbliene A. , Sasnauskas K. , Shiell B. , Russell G. , Bingham J. , Michalski W. P. . ( 2009; ). Generation of Tioman virus nucleocapsid-like particles in yeast Saccharomyces cerevisiae . . Virus Res 145:, 92–96. [CrossRef] [PubMed]
    [Google Scholar]
  14. Philbey A. W. , Kirkland P. D. , Ross A. D. , Davis R. J. , Gleeson A. B. , Love R. J. , Daniels P. W. , Gould A. R. , Hyatt A. D. . ( 1998; ). An apparently new virus (family Paramyxoviridae) infectious for pigs, humans, and fruit bats. . Emerg Infect Dis 4:, 269–271. [CrossRef] [PubMed]
    [Google Scholar]
  15. Philbey A. W. , Ross A. D. , Kirkland P. D. , Love R. J. . ( 2007; ). Skeletal and neurological malformations in pigs congenitally infected with Menangle virus. . Aust Vet J 85:, 134–140. [CrossRef] [PubMed]
    [Google Scholar]
  16. Philbey A. W. , Kirkland P. D. , Ross A. D. , Field H. E. , Srivastava M. , Davis R. J. , Love R. J. . ( 2008; ). Infection with Menangle virus in flying foxes (Pteropus spp.) in Australia. . Aust Vet J 86:, 449–454. [CrossRef] [PubMed]
    [Google Scholar]
  17. Utz J. P. , Szwed C. F. . ( 1962; ). Mumps III. Comparison of methods for detection of viruria. . Proc Soc Exp Biol Med 110:, 841–844.[PubMed] [CrossRef]
    [Google Scholar]
  18. Utz J. P. , Houk V. N. , Alling D. W. . ( 1964; ). Clinical and laboratory studies of mumps – viruria and abnormal renal function. . N Engl J Med 270:, 1283–1286. [CrossRef] [PubMed]
    [Google Scholar]
  19. Villegas P. . ( 1998; ). Titration of biological suspensions. . In A Laboratory Manual for the Isolation and Identification of Avian Pathogens, , 4th edn., pp. 248–254. Edited by Swayne D. E. , Glisson J. R. , Jackwood M. W. , Pearson J. E. , Reed W. M. . . Pennsylvania:: American Association of Avian Pathologists;.
    [Google Scholar]
  20. von Messling V. , Milosevic D. , Cattaneo R. . ( 2004; ). Tropism illuminated: lymphocyte-based pathways blazed by lethal morbillivirus through the host immune system. . Proc Natl Acad Sci U S A 101:, 14216–14221. [CrossRef] [PubMed]
    [Google Scholar]
  21. von Messling V. , Svitek N. , Cattaneo R. . ( 2006; ). Receptor (SLAM [CD150]) recognition and the V protein sustain swift lymphocyte-based invasion of mucosal tissue and lymphatic organs by a morbillivirus. . J Virol 80:, 6084–6092. [CrossRef] [PubMed]
    [Google Scholar]
  22. Yaiw K. C. , Bingham J. , Crameri G. , Mungall B. , Hyatt A. , Yu M. , Eaton B. , Shamala D. , Wang L. F. , Wong K. T. . ( 2008; ). Tioman virus, a paramyxovirus of bat origin, causes mild disease in pigs and has a predilection for lymphoid tissues. . J Virol 82:, 565–568. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.038448-0
Loading
/content/journal/jgv/10.1099/vir.0.038448-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error