1887

Abstract

We report the identification of a novel single-stranded, circular DNA virus isolated from bovine stool. The virus, named bovine stool-associated circular DNA virus (BoSCV), has a genome comprising 2600 bases of circular ssDNA, with two putative ORFs encoding replicase and capsid proteins, arranged inversely. The stem–loop structure was located between the 3′ ends of the two putative ORFs, as in chimpanzee stool-associated circular virus (ChimpSCV) and unlike other circular DNA viruses, including members of the families , and . BoSCV was also genetically similar to ChimpSCV, with approximately 30 % identity in the replicase and capsid proteins. A phylogenetic analysis based on the replicase protein showed that BoSCV and ChimpSCV are in the same clade. A field survey using BoSCV-specific PCRs targeting ORF1 detected BoSCV and BoSCV-like sequences in bovine and porcine stool samples. BoSCV appears to belong to a new genus of circular DNA viruses.

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/content/journal/jgv/10.1099/vir.0.037838-0
2012-03-01
2019-11-15
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References

  1. Bagge E., Persson M., Johansson K. E.. ( 2010;). Diversity of spore-forming bacteria in cattle manure, slaughterhouse waste and samples from biogas plants. . J Appl Microbiol 109:, 1549–1565.[PubMed]
    [Google Scholar]
  2. Blinkova O., Victoria J., Li Y., Keele B. F., Sanz C., Ndjango J.-B. N., Peeters M., Travis D., Lonsdorf E. V.. & other authors ( 2010;). Novel circular DNA viruses in stool samples of wild-living chimpanzees. . J Gen Virol 91:, 74–86. [CrossRef][PubMed]
    [Google Scholar]
  3. Colson P., Richet H., Desnues C., Balique F., Moal V., Grob J.-J., Berbis P., Lecoq H., Harlé J. R.. & other authors ( 2010;). Pepper mild mottle virus, a plant virus associated with specific immune responses, Fever, abdominal pains, and pruritus in humans. . PLoS One 5:, e10041. [CrossRef][PubMed]
    [Google Scholar]
  4. Delcher A. L., Harmon D., Kasif S., White O., Salzberg S. L.. ( 1999;). Improved microbial gene identification with glimmer. . Nucleic Acids Res 27:, 4636–4641. [CrossRef][PubMed]
    [Google Scholar]
  5. Ge X., Li J., Peng C., Wu L., Yang X., Wu Y., Zhang Y., Shi Z.. ( 2011;). Genetic diversity of novel circular ssDNA viruses in bats in China. . J Gen Virol 92:, 2646–2653. [CrossRef][PubMed]
    [Google Scholar]
  6. Hall T. A.. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.. Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  7. Huang X., Madan A.. ( 1999;). cap3: a DNA sequence assembly program. . Genome Res 9:, 868–877. [CrossRef][PubMed]
    [Google Scholar]
  8. Kim H. K., Moon H. J., Park S. J., Rho S. M., Han J. Y., Nguyen V. G., Park B. K.. ( 2011;). Identification of two cytopathogenic agents, Mycoplasma hyorhinis and mammalian orthoreovirus 3 based on modified particle associated nucleic acids PCR. . Korean J Vet Res 51:, 129–137.
    [Google Scholar]
  9. Letellier C., Kerkhofs P., Wellemans G., Vanopdenbosch E.. ( 1999;). Detection and genotyping of bovine diarrhea virus by reverse transcription-polymerase chain amplification of the 5′ untranslated region. . Vet Microbiol 64:, 155–167. [CrossRef][PubMed]
    [Google Scholar]
  10. Li L., Kapoor A., Slikas B., Bamidele O. S., Wang C., Shaukat S., Masroor M. A., Wilson M. L., Ndjango J.-B. N.. & other authors ( 2010;). Multiple diverse circoviruses infect farm animals and are commonly found in human and chimpanzee feces. . J Virol 84:, 1674–1682. [CrossRef][PubMed]
    [Google Scholar]
  11. Li L., Shan T., Soji O. B., Alam M. M., Kunz T. H., Zaidi S. Z., Delwart E.. ( 2011;). Possible cross-species transmission of circoviruses and cycloviruses among farm animals. . J Gen Virol 92:, 768–772. [CrossRef][PubMed]
    [Google Scholar]
  12. Lőrincz M., Cságola A., Farkas S. L., Székely C., Tuboly T.. ( 2011;). First detection and analysis of a fish circovirus. . J Gen Virol 92:, 1817–1821. [CrossRef][PubMed]
    [Google Scholar]
  13. Okamoto H.. ( 2009;). TT viruses in animals. . Curr Top Microbiol Immunol 331:, 35–52. [CrossRef][PubMed]
    [Google Scholar]
  14. Papadopoulos J. S., Agarwala R.. ( 2007;). cobalt: constraint-based alignment tool for multiple protein sequences. . Bioinformatics 23:, 1073–1079. [CrossRef][PubMed]
    [Google Scholar]
  15. Rosario K., Marinov M., Stainton D., Kraberger S., Wiltshire E. J., Collings D. A., Walters M., Martin D. P., Breitbart M., Varsani A.. ( 2011;). Dragonfly cyclovirus, a novel single-stranded DNA virus discovered in dragonflies (Odonata: Anisoptera). . J Gen Virol 92:, 1302–1308. [CrossRef][PubMed]
    [Google Scholar]
  16. Stang A., Korn K., Wildner O., Uberla K.. ( 2005;). Characterization of virus isolates by particle-associated nucleic acid PCR. . J Clin Microbiol 43:, 716–720. [CrossRef][PubMed]
    [Google Scholar]
  17. Takiuchi E., Stipp D. T., Alfieri A. F., Alfieri A. A.. ( 2006;). Improved detection of bovine coronavirus N gene in faeces of calves infected naturally by a semi-nested PCR assay and an internal control. . J Virol Methods 131:, 148–154. [CrossRef][PubMed]
    [Google Scholar]
  18. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  20. Todd D.. ( 2005;). Circoviridae. San Diego, CA:: Elsevier Academic Press;.
    [Google Scholar]
  21. Todd D., McNulty M. S., Adair B. M., Allan G. M.. ( 2001;). Animal circoviruses. . Adv Virus Res 57:, 1–70. [CrossRef][PubMed]
    [Google Scholar]
  22. Yang J. S., Song D. S., Kim S. Y., Lyoo K. S., Park B. K.. ( 2003;). Detection of porcine circovirus type 2 in feces of pigs with or without enteric disease by polymerase chain reaction. . J Vet Diagn Invest 15:, 369–373. [CrossRef][PubMed]
    [Google Scholar]
  23. Zhang T., Breitbart M., Lee W. H., Run J.-Q., Wei C. L., Soh S. W. L., Hibberd M. L., Liu E. T., Rohwer F., Ruan Y.. ( 2006;). RNA viral community in human feces: prevalence of plant pathogenic viruses. . PLoS Biol 4:, e3. [CrossRef][PubMed]
    [Google Scholar]
  24. Zuker M.. ( 2003;). Mfold web server for nucleic acid folding and hybridization prediction. . Nucleic Acids Res 31:, 3406–3415. [CrossRef][PubMed]
    [Google Scholar]
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