1887

Abstract

Porcine rotavirus B (RVB) has frequently been detected in diarrhoea of suckling and weaned pigs. Moreover, epidemiological studies using ELISA have demonstrated high antibody prevalence in sera from sows, indicating that RVB infections are widespread. Because it is difficult to propagate RVBs serially in cell culture, genetic analysis of RNA segments of porcine RVBs other than those encoding VP7 and NSP2 has been scarcely performed. We conducted sequence and phylogenetic analyses focusing on non-structural protein 1 (NSP1), using 15 porcine RVB strains isolated from diarrhoeic faeces collected around Japan. Sequence analysis showed that the porcine NSP1 gene contains two overlapping ORFs. Especially, peptide 2 of NSP1 retains highly conserved cysteine and histidine residues among RVBs. Comparison of NSP1 nucleotide and deduced amino acid sequences from porcine RVB strains demonstrated low identities to those from other RVB strains. Phylogenetic analysis of RVB NSP1 revealed the presence of murine, human, ovine, bovine and porcine clusters. Furthermore, the NSP1 genes of porcine RVBs were divided into three genotypes, suggesting the possibility that porcine species might be an original host of RVB infection. Of nine strains common to those used in our previous study, only one strain was classified into a different genotype from the others in the analysis of VP7, in contrast to the analysis of NSP1, where all belonged to the same cluster. This fact suggests the occurrence of gene reassortment among porcine RVBs. These findings should provide more beneficent information to understand the evolution and functions of RVBs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.036426-0
2011-12-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/92/12/2922.html?itemId=/content/journal/jgv/10.1099/vir.0.036426-0&mimeType=html&fmt=ahah

References

  1. Alam M. M. , Kobayashi N. , Ishino M. , Ahmed M. S. , Ahmed M. U. , Paul S. K. , Muzumdar B. K. , Hussain Z. , Wang Y. H. , Naik T. N. . ( 2007; ). Genetic analysis of an ADRV-N-like novel rotavirus strain B219 detected in a sporadic case of adult diarrhea in Bangladesh. . Arch Virol 152:, 199–208. [CrossRef] [PubMed]
    [Google Scholar]
  2. Arnold M. M. , Patton J. T. . ( 2011; ). Diversity of interferon antagonist activities mediated by NSP1 proteins of different rotavirus strains. . J Virol 85:, 1970–1979. [CrossRef] [PubMed]
    [Google Scholar]
  3. Aung T. S. , Kobayashi N. , Nagashima S. , Ghosh S. , Aung M. S. , Oo K. Y. , Win N. . ( 2009; ). Detection of group B rotavirus in an adult with acute gastroenteritis in Yangon, Myanmar. . J Med Virol 81:, 1968–1974. [CrossRef] [PubMed]
    [Google Scholar]
  4. Barman P. , Ghosh S. , Das S. , Varghese V. , Chaudhuri S. , Sarkar S. , Krishnan T. , Bhattacharya S. K. , Chakrabarti A. et al. ( 2004; ). Sequencing and sequence analysis of VP7 and NSP5 genes reveal emergence of a new genotype of bovine group B rotaviruses in India. . J Clin Microbiol 42:, 2816–2818. [CrossRef] [PubMed]
    [Google Scholar]
  5. Barro M. , Patton J. T. . ( 2005; ). Rotavirus nonstructural protein 1 subverts innate immune response by inducing degradation of IFN regulatory factor 3. . Proc Natl Acad Sci U S A 102:, 4114–4119. [CrossRef] [PubMed]
    [Google Scholar]
  6. Barro M. , Patton J. T. . ( 2007; ). Rotavirus NSP1 inhibits expression of type I interferon by antagonizing the function of interferon regulatory factors IRF3, IRF5, and IRF7. . J Virol 81:, 4473–4481. [CrossRef] [PubMed]
    [Google Scholar]
  7. Brown D. W. , Beards G. M. , Chen G. M. , Flewett T. H. . ( 1987; ). Prevalence of antibody to group B (atypical) rotavirus in humans and animals. . J Clin Microbiol 25:, 316–319.[PubMed]
    [Google Scholar]
  8. Chang K. O. , Parwani A. V. , Smith D. , Saif L. J. . ( 1997; ). Detection of group B rotaviruses in fecal samples from diarrheic calves and adult cows and characterization of their VP7 genes. . J Clin Microbiol 35:, 2107–2110.[PubMed]
    [Google Scholar]
  9. Chen C. M. , Hung T. , Bridger J. C. , McCrae M. A. . ( 1985; ). Chinese adult rotavirus is a group B rotavirus. . Lancet 2:, 1123–1124. [CrossRef] [PubMed]
    [Google Scholar]
  10. Dunn S. J. , Cross T. L. , Greenberg H. B. . ( 1994; ). Comparison of the rotavirus nonstructural protein NSP1 (NS53) from different species by sequence analysis and northern blot hybridization. . Virology 203:, 178–183. [CrossRef] [PubMed]
    [Google Scholar]
  11. Eiden J. J. , Nataro J. , Vonderfecht S. , Petric M. . ( 1992; ). Molecular cloning, sequence analysis, in vitro expression, and immunoprecipitation of the major inner capsid protein of the IDIR strain of group B rotavirus (GBR). . Virology 188:, 580–589. [CrossRef] [PubMed]
    [Google Scholar]
  12. Ghosh S. , Varghese V. , Sinha M. , Kobayashi N. , Naik T. N. . ( 2007; ). Evidence for interstate transmission and increase in prevalence of bovine group B rotavirus strains with a novel VP7 genotype among diarrhoeic calves in Eastern and Northern states of India. . Epidemiol Infect 135:, 1324–1330. [CrossRef] [PubMed]
    [Google Scholar]
  13. Ghosh S. , Kobayashi N. , Nagashima S. , Chawla-Sarkar M. , Krishnan T. , Ganesh B. , Naik T. N. . ( 2010; ). Molecular characterization of the VP1, VP2, VP4, VP6, NSP1 and NSP2 genes of bovine group B rotaviruses: identification of a novel VP4 genotype. . Arch Virol 155:, 159–167. [CrossRef] [PubMed]
    [Google Scholar]
  14. Graff J. W. , Mitzel D. N. , Weisend C. M. , Flenniken M. L. , Hardy M. E. . ( 2002; ). Interferon regulatory factor 3 is a cellular partner of rotavirus NSP1. . J Virol 76:, 9545–9550. [CrossRef] [PubMed]
    [Google Scholar]
  15. Graff J. W. , Ewen J. , Ettayebi K. , Hardy M. E. . ( 2007; ). Zinc-binding domain of rotavirus NSP1 is required for proteasome-dependent degradation of IRF3 and autoregulatory NSP1 stability. . J Gen Virol 88:, 613–620. [CrossRef] [PubMed]
    [Google Scholar]
  16. Graff J. W. , Ettayebi K. , Hardy M. E. . ( 2009; ). Rotavirus NSP1 inhibits NFκB activation by inducing proteasome-dependent degradation of β-TrCP: a novel mechanism of IFN antagonism. . PLoS Pathog 5:, e1000280. [CrossRef] [PubMed]
    [Google Scholar]
  17. Hua J. , Patton J. T. . ( 1994; ). The carboxyl-half of the rotavirus nonstructural protein NS53 (NSP1) is not required for virus replication. . Virology 198:, 567–576. [CrossRef] [PubMed]
    [Google Scholar]
  18. Hua J. , Mansell E. A. , Patton J. T. . ( 1993; ). Comparative analysis of the rotavirus NS53 gene: conservation of basic and cysteine-rich regions in the protein and possible stem-loop structures in the RNA. . Virology 196:, 372–378. [CrossRef] [PubMed]
    [Google Scholar]
  19. Hundley F. , Biryahwaho B. , Gow M. , Desselberger U. . ( 1985; ). Genome rearrangements of bovine rotavirus after serial passage at high multiplicity of infection. . Virology 143:, 88–103. [CrossRef] [PubMed]
    [Google Scholar]
  20. Hung T. , Chen G. M. , Wang C. G. , Yao H. L. , Fang Z. Y. , Chao T. X. , Chou Z. Y. , Ye W. , Chang X. J. et al. ( 1984; ). Waterborne outbreak of rotavirus diarrhoea in adults in China caused by a novel rotavirus. . Lancet 1:, 1139–1142.[PubMed]
    [Google Scholar]
  21. James V. L. A. , Lambden P. R. , Deng Y. , Caul E. O. , Clarke I. N. . ( 1999; ). Molecular characterization of human group C rotavirus genes 6, 7 and 9. . J Gen Virol 80:, 3181–3187.[PubMed]
    [Google Scholar]
  22. Janke B. H. , Nelson J. K. , Benfield D. A. , Nelson E. A. . ( 1990; ). Relative prevalence of typical and atypical strains among rotaviruses from diarrheic pigs in conventional swine herds. . J Vet Diagn Invest 2:, 308–311. [CrossRef] [PubMed]
    [Google Scholar]
  23. Jiang S. , Ji S. , Tang Q. , Cui X. , Yang H. , Kan B. , Gao S. . ( 2008; ). Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses. . J Gen Virol 89:, 2622–2629. [CrossRef] [PubMed]
    [Google Scholar]
  24. Kelkar S. D. , Zade J. K. . ( 2004; ). Group B rotaviruses similar to strain CAL-1, have been circulating in Western India since 1993. . Epidemiol Infect 132:, 745–749. [CrossRef] [PubMed]
    [Google Scholar]
  25. Kobayashi N. , Naik T. N. , Kusuhara Y. , Krishnan T. , Sen A. , Bhattacharya S. K. , Taniguchi K. , Alam M. M. , Urasawa T. , Urasawa S. . ( 2001; ). Sequence analysis of genes encoding structural and nonstructural proteins of a human group B rotavirus detected in Calcutta, India. . J Med Virol 64:, 583–588. [CrossRef] [PubMed]
    [Google Scholar]
  26. Kobayashi N. , Alam M. , Kojima K. , Mise K. , Ishino M. , Sumi A. . ( 2003; ). Genomic diversity and evolution of rotaviruses: an overview. . In Genomic Diversity and Molecular Epidemiology of Rotaviruses, pp. 75–90. Edited by Kobayashi N. . . Trivandrum, India:: Research Signpost;.
    [Google Scholar]
  27. Kojima K. , Taniguchi K. , Kobayashi N. . ( 1996; ). Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, bovine, feline, and equine rotavirus strains. . Arch Virol 141:, 1–12. [CrossRef] [PubMed]
    [Google Scholar]
  28. Krishnan T. , Sen A. , Choudhury J. S. , Das S. , Naik T. N. , Bhattacharya S. K. . ( 1999; ). Emergence of adult diarrhoea rotavirus in Calcutta, India. . Lancet 353:, 380–381. [CrossRef] [PubMed]
    [Google Scholar]
  29. Kuga K. , Miyazaki A. , Suzuki T. , Takagi M. , Hattori N. , Katsuda K. , Mase M. , Sugiyama M. , Tsunemitsu H. . ( 2009; ). Genetic diversity and classification of the outer capsid glycoprotein VP7 of porcine group B rotaviruses. . Arch Virol 154:, 1785–1795. [CrossRef] [PubMed]
    [Google Scholar]
  30. Lambden P. R. , Cooke S. J. , Caul E. O. , Clarke I. N. . ( 1992; ). Cloning of noncultivatable human rotavirus by single primer amplification. . J Virol 66:, 1817–1822.[PubMed]
    [Google Scholar]
  31. Maan S. , Rao S. , Maan N. S. , Anthony S. J. , Attoui H. , Samuel A. R. , Mertens P. P. . ( 2007; ). Rapid cDNA synthesis and sequencing techniques for the genetic study of bluetongue and other dsRNA viruses. . J Virol Methods 143:, 132–139. [CrossRef] [PubMed]
    [Google Scholar]
  32. Matthijnssens J. , Ciarlet M. , Heiman E. , Arijs I. , Delbeke T. , McDonald S. M. , Palombo E. A. , Iturriza-Gómara M. , Maes P. et al. ( 2008a; ). Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. . J Virol 82:, 3204–3219. [CrossRef] [PubMed]
    [Google Scholar]
  33. Matthijnssens J. , Ciarlet M. , Rahman M. , Attoui H. , Bányai K. , Estes M. K. , Gentsch J. R. , Iturriza-Gómara M. , Kirkwood C. D. et al. ( 2008b; ). Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. . Arch Virol 153:, 1621–1629. [CrossRef] [PubMed]
    [Google Scholar]
  34. Pesavento J. B. , Crawford S. E. , Estes M. K. , Prasad B. V. . ( 2006; ). Rotavirus proteins: structure and assembly. . Curr Top Microbiol Immunol 309:, 189–219. [CrossRef] [PubMed]
    [Google Scholar]
  35. Petric M. , Mayur K. , Vonderfecht S. , Eiden J. J. . ( 1991; ). Comparison of group B rotavirus genes 9 and 11. . J Gen Virol 72:, 2801–2804. [CrossRef] [PubMed]
    [Google Scholar]
  36. Potgieter A. C. , Steele A. D. , van Dijk A. A. . ( 2002; ). Cloning of complete genome sets of six dsRNA viruses using an improved cloning method for large dsRNA genes. . J Gen Virol 83:, 2215–2223.[PubMed]
    [Google Scholar]
  37. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  38. Sanekata T. , Kuwamoto Y. , Akamatsu S. , Sakon N. , Oseto M. , Taniguchi K. , Nakata S. , Estes M. K. . ( 1996; ). Isolation of group B porcine rotavirus in cell culture. . J Clin Microbiol 34:, 759–761.[PubMed]
    [Google Scholar]
  39. Sanekata T. , Ahmed M. U. , Kader A. , Taniguchi K. , Kobayashi N. . ( 2003; ). Human group B rotavirus infections cause severe diarrhea in children and adults in Bangladesh. . J Clin Microbiol 41:, 2187–2190. [CrossRef] [PubMed]
    [Google Scholar]
  40. Shen S. , McKee T. A. , Wang Z. D. , Desselberger U. , Liu D. X. . ( 1999; ). Sequence analysis and in vitro expression of genes 6 and 11 of an ovine group B rotavirus isolate, KB63: evidence for a non-defective, C-terminally truncated NSP1 and a phosphorylated NSP5. . J Gen Virol 80:, 2077–2085.[PubMed]
    [Google Scholar]
  41. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739.[CrossRef]
    [Google Scholar]
  42. Taniguchi K. , Kojima K. , Urasawa S. . ( 1996; ). Nondefective rotavirus mutants with an NSP1 gene which has a deletion of 500 nucleotides, including a cysteine-rich zinc finger motif-encoding region (nucleotides 156 to 248), or which has a nonsense codon at nucleotides 153 to 155. . J Virol 70:, 4125–4130.[PubMed]
    [Google Scholar]
  43. Theil K. W. , Saif L. J. , Moorhead P. D. , Whitmoyer R. E. . ( 1985; ). Porcine rotavirus-like virus (group B rotavirus): characterization and pathogenicity for gnotobiotic pigs. . J Clin Microbiol 21:, 340–345.[PubMed]
    [Google Scholar]
  44. Tsunemitsu H. , Jiang B. , Yamashita Y. , Oseto M. , Ushijima H. , Saif L. . ( 1992; ). Evidence of serologic diversity within group C rotaviruses. . J Clin Microbiol 30:, 3009–3012.
    [Google Scholar]
  45. Tsunemitsu H. , Morita D. , Takaku H. , Nishimori T. , Imai K. , Saif L. J. . ( 1999; ). First detection of bovine group B rotavirus in Japan and sequence of its VP7 gene. . Arch Virol 144:, 805–815. [CrossRef] [PubMed]
    [Google Scholar]
  46. Tsunemitsu H. , Kamiyama M. , Kawashima K. , Katsuda K. , Kohmoto M. , Saif L. J. , Shouji T. , Onodera T. . ( 2005; ). Molecular characterization of the major capsid protein VP6 of bovine group B rotavirus and its use in seroepidemiology. . J Gen Virol 86:, 2569–2575. [CrossRef] [PubMed]
    [Google Scholar]
  47. Wakuda M. , Pongsuwanna Y. , Taniguchi K. . ( 2005; ). Complete nucleotide sequences of two RNA segments of human picobirnavirus. . J Virol Methods 126:, 165–169. [CrossRef] [PubMed]
    [Google Scholar]
  48. Yamamoto D. , Ghosh S. , Ganesh B. , Krishnan T. , Chawla-Sarkar M. , Alam M. M. , Aung T. S. , Kobayashi N. . ( 2010; ). Analysis of genetic diversity and molecular evolution of human group B rotaviruses based on whole genome segments. . J Gen Virol 91:, 1772–1781. [CrossRef] [PubMed]
    [Google Scholar]
  49. Yang H. , Makeyev E. V. , Kang Z. , Ji S. , Bamford D. H. , van Dijk A. A. . ( 2004; ). Cloning and sequence analysis of dsRNA segments 5, 6 and 7 of a novel non-group A, B, C adult rotavirus that caused an outbreak of gastroenteritis in China. . Virus Res 106:, 15–26. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.036426-0
Loading
/content/journal/jgv/10.1099/vir.0.036426-0
Loading

Data & Media loading...

Supplements

vol. , part 12, pp.2922–2929

Alignment of predicted amino acid sequences of NSP1 peptides 1 and 2 from representative RVB strains

Origin of 15 porcine RVBs in faecal samples from suckling and weaned pigs

[ Single PDF file] (647 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error