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Journal of General Virology header logo Journal of General Virology

Volume 93, Issue 2

The 5′ leader sequence of mouse mammary tumor virus enhances expression of the envelope and reporter genes No Access

Abstract

Mouse mammary tumor virus (MMTV) is a complex betaretrovirus, which utilizes a Rev-like auxiliary protein Rem to export the unspliced viral RNA from the nucleus. MMTV mRNA appears to be exported via a distinct, Rem-independent, mechanism. Here, we analysed the effect of an extensively folded region coinciding with the 5′ leader sequence on gene expression. We found that the presence of the 5′ leader stimulates expression of the envelope protein. Enhanced Env production was accompanied by increased cytoplasmic levels of mRNA. The 5′ leader promotes nucleocytoplasmic translocation and increases stability of mRNA. The region responsible for this effect was mapped to the distal part of the 5′ leader. Furthermore, the 5′ leader inserted in the sense orientation into a heterologous luciferase expression construct increased luciferase activity.

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© 2012 SGM
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/content/journal/jgv/10.1099/vir.0.035196-0
2012-02-01
2025-04-26
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The 5′ leader sequence of mouse mammary tumor virus enhances expression of the envelope and reporter genes
J. Gen. Virol. 93, 308 (2012); https://doi.org/10.1099/vir.0.035196-0
/content/journal/jgv/10.1099/vir.0.035196-0
/content/journal/jgv/10.1099/vir.0.035196-0
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