RT Journal Article SR Electronic(1) A1 Ito, Yoshinori A1 Kawabe, Shinji A1 Kojima, Seiji A1 Nakamura, Fumihiko A1 Nishiyama, Yukihiro A1 Kaneko, Kenichiro A1 Kiuchi, Tetsuya A1 Ando, Hisami A1 Kimura, HiroshiYR 2011 T1 Identification of Epstein–Barr virus-infected CD27+ memory B-cells in liver or stem cell transplant patients JF Journal of General Virology, VO 92 IS 11 SP 2590 OP 2595 DO https://doi.org/10.1099/vir.0.033712-0 PB Microbiology Society, SN 1465-2099, AB To analyse the phenotype of Epstein–Barr virus (EBV)-infected lymphocytes in EBV-associated infections, cells from eight haematopoietic stem cell/liver transplantation recipients with elevated EBV viral loads were examined by a novel quantitative assay designed to identify EBV-infected cells by using a flow cytometric detection of fluorescent in situ hybridization (FISH) assay. By this assay, 0.05–0.78 % of peripheral blood lymphocytes tested positive for EBV, and the EBV-infected cells were CD20+ B-cells in all eight patients. Of the CD20+ EBV-infected lymphocytes, 48–83 % of cells tested IgD positive and 49–100 % of cells tested CD27 positive. Additionally, the number of EBV-infected cells assayed by using FISH was significantly correlated with the EBV-DNA load, as determined by real-time PCR (r 2 = 0.88, P<0.0001). The FISH assay enabled us to characterize EBV-infected cells and perform a quantitative analysis in patients with EBV infection after stem cell/liver transplantation., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.033712-0