1887

Abstract

Ankyrin-repeat (ANK) protein-interaction domains are common in cellular proteins but are relatively rare in viruses. Chordopoxviruses, however, encode a large number of ANK domain-containing ORFs of largely unknown function. Recently, a second protein-interaction domain, an F-box-like motif, was identified in several poxvirus ANK proteins. Cellular F-box proteins recruit substrates to the ubiquitination machinery of the cell, a putative function for ANK/poxviral F-box proteins. Using publicly available genome sequence data we examined all 328 predicted ANK proteins encoded by 27 chordopoxviruses that represented the eight vertebrate poxvirus genera whose members encode ANK proteins. Within these we identified 15 putative ANK protein orthologue groups within orthopoxviruses, five within parapoxviruses, 23 within avipoxviruses and seven across members of the genera , , , and . Sequence comparisons showed that members of each of these four clusters of orthologues were not closely related to members of any of the other clusters. Of these ORFs, 67 % encoded a C-terminal poxviral F-box-like motif, whose absence could largely be attributed to fragmentation of ORFs. Our findings suggest that the large family of poxvirus ANK proteins arose by extensive gene duplication and divergence that occurred independently in four major genus-based groups after the groups diverged from each other. It seems likely that the ancestor ANK proteins of poxviruses contained both the N-terminal ANK repeats and a C-terminal F-box-like domain, with the latter domain subsequently being lost in a small subset of these proteins.

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2011-11-01
2019-10-19
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vol. , part 11, pp. 2596 - 2607

The ANK proteins of chordopoxviruses form four major groups reflecting the inter-genus relationships of the subfamily

Phylogenetic relationship of 181 ANK proteins of orthopoxviruses

Phylogenetic relationship of 82 ANK proteins of avipoxviruses

F-box-like motifs identified in 219 of 328 examined chordopoxvirus ANK proteins

ANK protein ORF of chordopoxviruses used in this study

Seven putative ANK protein orthologue groups of LSG

Amino acid sequence identities of poxvirus ANK proteins within and across the LSG genera

Amino acid sequence identities of poxvirus ANK proteins within LSG orthologue groups

Parapoxvirus ANK proteins form seven orthologue groups

Orthopoxvirus (OPV) ANK protein orthologue groups [Single PDF file](3864 KB)



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