1887

Abstract

Although G2P[4] rotaviruses are common causes of acute childhood diarrhoea in Africa, to date there are no reports on whole genomic analysis of African G2P[4] strains. In this study, the nearly complete genome sequences of two Kenyan G2P[4] strains, AK26 and D205, detected in 1982 and 1989, respectively, were analysed. Strain D205 exhibited a DS-1-like genotype constellation, whilst strain AK26 appeared to be an intergenogroup reassortant with a Wa-like NSP2 genotype on the DS-1-like genotype constellation. The VP2-4, VP6-7, NSP1, NSP3 and NSP5 genes of strain AK26 and the VP2, VP4, VP7 and NSP1–5 genes of strain D205 were closely related to those of the prototype or other human G2P[4] strains. In contrast, their remaining genes were distantly related, and, except for NSP2 of AK26, appeared to originate from or share a common origin with rotavirus genes of artiodactyl (ruminant and camelid) origin. These observations highlight the complex evolutionary dynamics of African G2P[4] rotaviruses.

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2011-09-01
2019-10-18
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vol. , part 8, pp. 2201–2208

Comparison of the deduced amino acid sequences of the VP7 genes of strains AK26 and D205 with those of other old and recent African G2 strains, prototype G2P[4] strain DS-1, recent G2P[4] strains MMC6, MMC88 and LB2772, and the G2 component of rotavirus vaccine, RotaTeq.

Primers used for amplification of different genes of group A rotavirus strains AK26 and D205

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