%0 Journal Article %A Veldhuis Kroeze, Edwin J. B. %A van Amerongen, Geert %A Dijkshoorn, Marcel L. %A Simon, James H. %A de Waal, Leon %A Hartmann, Ieneke J. C. %A Krestin, Gabriel P. %A Kuiken, Thijs %A Osterhaus, Albert D. M. E. %A Stittelaar, Koert J. %T Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography %D 2011 %J Journal of General Virology, %V 92 %N 8 %P 1854-1858 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.032805-0 %I Microbiology Society, %X We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning in all infected lungs corresponded to areas of alveolar oedema at necropsy. These areas were most pronounced on day 3 and gradually decreased from days 4 to 7 post-infection. This pilot study shows that the non-invasive imaging procedure allows quantification and characterization of influenza-induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in pre-clinical pharmaceutical efficacy studies. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.032805-0