1887

Abstract

We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning in all infected lungs corresponded to areas of alveolar oedema at necropsy. These areas were most pronounced on day 3 and gradually decreased from days 4 to 7 post-infection. This pilot study shows that the non-invasive imaging procedure allows quantification and characterization of influenza-induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in pre-clinical pharmaceutical efficacy studies.

  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2011-08-01
2024-03-28
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References

  1. Baras B., de Waal L., Stittelaar K. J., Jacob V., Giannini S., Veldhuis Kroeze E. J., van den Brand J. M. A., van Amerongen G., Simon J. H. et al. 2011; Pandemic H1N1 vaccine requires the use of an adjuvant to protect against challenge in naïve ferrets. Vaccine 29:2120–2126 [View Article][PubMed]
    [Google Scholar]
  2. CDC 2009; Update: infections with a swine-origin influenza A (H1N1) virus–United States and other countries, April 28, 2009. MMWR Morb Mortal Wkly Rep 58:431–433[PubMed]
    [Google Scholar]
  3. Del Giudice G., Stittelaar K. J., van Amerongen G., Simon J., Osterhaus A. D., Stöhr K., Rappuoli R. 2009; Seasonal influenza vaccine provides priming for A/H1N1 immunization. Sci Transl Med 1:re1[PubMed] [CrossRef]
    [Google Scholar]
  4. Friesen R. H., Koudstaal W., Koldijk M. H., Weverling G. J., Brakenhoff J. P., Lenting P. J., Stittelaar K. J., Osterhaus A. D., Kompier R., Goudsmit J. 2010; New class of monoclonal antibodies against severe influenza: prophylactic and therapeutic efficacy in ferrets. PLoS ONE 5:e9106 [View Article][PubMed]
    [Google Scholar]
  5. Gill J. R., Sheng Z. M., Ely S. F., Guinee D. G., Beasley M. B., Suh J., Deshpande C., Mollura D. J., Morens D. M. et al. 2010; Pulmonary pathologic findings of fatal 2009 pandemic influenza A/H1N1 viral infections. Arch Pathol Lab Med 134:235–243[PubMed]
    [Google Scholar]
  6. Itoh Y., Shinya K., Kiso M., Watanabe T., Sakoda Y., Hatta M., Muramoto Y., Tamura D., Sakai-Tagawa Y. et al. 2009; In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses. Nature 460:1021–1025[PubMed]
    [Google Scholar]
  7. Kärber G. 1931; Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch Exp Path Pharmak 162:480–483 (in German) [View Article]
    [Google Scholar]
  8. Li P., Su D. J., Zhang J. F., Xia X. D., Sui H., Zhao D. H. 2010; Pneumonia in novel swine-origin influenza A (H1N1) virus infection: high-resolution CT findings. Eur J Radiol [View Article][PubMed]
    [Google Scholar]
  9. Marchiori E., Zanetti G., Hochhegger B., Rodrigues R. S., Fontes C. A., Nobre L. F., Mançano A. D., Meirelles G., Irion K. L. 2010; High-resolution computed tomography findings from adult patients with influenza A (H1N1) virus-associated pneumonia. Eur J Radiol 74:93–98 [View Article][PubMed]
    [Google Scholar]
  10. Mollura D. J., Asnis D. S., Crupi R. S., Conetta R., Feigin D. S., Bray M., Taubenberger J. K., Bluemke D. A. 2009; Imaging findings in a fatal case of pandemic swine-origin influenza A (H1N1). AJR Am J Roentgenol 193:1500–1503 [View Article][PubMed]
    [Google Scholar]
  11. Munster V. J., de Wit E., van den Brand J. M., Herfst S., Schrauwen E. J., Bestebroer T. M., van de Vijver D., Boucher C. A., Koopmans M. et al. 2009; Pathogenesis and transmission of swine-origin 2009 A(H1N1) influenza virus in ferrets. Science 325:481–483[PubMed]
    [Google Scholar]
  12. Osterhaus A. D. 2008; New respiratory viruses of humans. Pediatr Infect Dis J 27:Suppl.S71–S74 [View Article][PubMed]
    [Google Scholar]
  13. Perez-Padilla R., de la Rosa-Zamboni D., Ponce de Leon S., Hernandez M., Quiñones-Falconi F., Bautista E., Ramirez-Venegas A., Rojas-Serrano J., Ormsby C. E. et al. 2009; Pneumonia and respiratory failure from swine-origin influenza A (H1N1) in Mexico. N Engl J Med 361:680–689 [View Article][PubMed]
    [Google Scholar]
  14. van den Brand J. M., Stittelaar K. J., van Amerongen G., Rimmelzwaan G. F., Simon J., de Wit E., Munster V., Bestebroer T., Fouchier R. A. et al. 2010; Severity of pneumonia due to new H1N1 influenza virus in ferrets is intermediate between that due to seasonal H1N1 virus and highly pathogenic avian influenza H5N1 virus. J Infect Dis 201:993–999 [View Article][PubMed]
    [Google Scholar]
  15. van den Brand J. M., Kreijtz J. H., Bodewes R., Stittelaar K. J., van Amerongen G., Kuiken T., Simon J., Fouchier R. A., Del Giudice G. et al. 2011; Efficacy of vaccination with different combinations of MF59-adjuvanted and nonadjuvanted seasonal and pandemic influenza vaccines against pandemic H1N1 (2009) influenza virus infection in ferrets. J Virol 85:2851–2858 [View Article][PubMed]
    [Google Scholar]
  16. van Riel D., Munster V. J., de Wit E., Rimmelzwaan G. F., Fouchier R. A., Osterhaus A. D., Kuiken T. 2007; Human and avian influenza viruses target different cells in the lower respiratory tract of humans and other mammals. Am J Pathol 171:1215–1223 [View Article][PubMed]
    [Google Scholar]
  17. Zhou B., Li Y., Belser J. A., Pearce M. B., Schmolke M., Subba A. X., Shi Z., Zaki S. R., Blau D. M., García-Sastre A. 2010; NS-based live attenuated H1N1 pandemic vaccines protect mice and ferrets. Vaccine 28:8015–8025 [View Article][PubMed]
    [Google Scholar]
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