1887

Abstract

We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning in all infected lungs corresponded to areas of alveolar oedema at necropsy. These areas were most pronounced on day 3 and gradually decreased from days 4 to 7 post-infection. This pilot study shows that the non-invasive imaging procedure allows quantification and characterization of influenza-induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in pre-clinical pharmaceutical efficacy studies.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.032805-0
2011-08-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/92/8/1854.html?itemId=/content/journal/jgv/10.1099/vir.0.032805-0&mimeType=html&fmt=ahah

References

  1. Baras B. , de Waal L. , Stittelaar K. J. , Jacob V. , Giannini S. , Veldhuis Kroeze E. J. , van den Brand J. M. A. , van Amerongen G. , Simon J. H. et al. ( 2011; ). Pandemic H1N1 vaccine requires the use of an adjuvant to protect against challenge in naïve ferrets. . Vaccine 29:, 2120–2126. [CrossRef].[PubMed]
    [Google Scholar]
  2. CDC ( 2009; ). Update: infections with a swine-origin influenza A (H1N1) virus–United States and other countries, April 28, 2009. . MMWR Morb Mortal Wkly Rep 58:, 431–433.[PubMed]
    [Google Scholar]
  3. Del Giudice G. , Stittelaar K. J. , van Amerongen G. , Simon J. , Osterhaus A. D. , Stöhr K. , Rappuoli R. . ( 2009; ). Seasonal influenza vaccine provides priming for A/H1N1 immunization. . Sci Transl Med 1:, re1.[PubMed] [CrossRef]
    [Google Scholar]
  4. Friesen R. H. , Koudstaal W. , Koldijk M. H. , Weverling G. J. , Brakenhoff J. P. , Lenting P. J. , Stittelaar K. J. , Osterhaus A. D. , Kompier R. , Goudsmit J. . ( 2010; ). New class of monoclonal antibodies against severe influenza: prophylactic and therapeutic efficacy in ferrets. . PLoS ONE 5:, e9106. [CrossRef].[PubMed]
    [Google Scholar]
  5. Gill J. R. , Sheng Z. M. , Ely S. F. , Guinee D. G. , Beasley M. B. , Suh J. , Deshpande C. , Mollura D. J. , Morens D. M. et al. ( 2010; ). Pulmonary pathologic findings of fatal 2009 pandemic influenza A/H1N1 viral infections. . Arch Pathol Lab Med 134:, 235–243.[PubMed]
    [Google Scholar]
  6. Itoh Y. , Shinya K. , Kiso M. , Watanabe T. , Sakoda Y. , Hatta M. , Muramoto Y. , Tamura D. , Sakai-Tagawa Y. et al. ( 2009; ). In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses. . Nature 460:, 1021–1025.[PubMed]
    [Google Scholar]
  7. Kärber G. . ( 1931; ). Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. . Arch Exp Path Pharmak 162:, 480–483 (in German). [CrossRef]
    [Google Scholar]
  8. Li P. , Su D. J. , Zhang J. F. , Xia X. D. , Sui H. , Zhao D. H. . ( 2010; ). Pneumonia in novel swine-origin influenza A (H1N1) virus infection: high-resolution CT findings. . Eur J Radiol. [CrossRef].[PubMed]
    [Google Scholar]
  9. Marchiori E. , Zanetti G. , Hochhegger B. , Rodrigues R. S. , Fontes C. A. , Nobre L. F. , Mançano A. D. , Meirelles G. , Irion K. L. . ( 2010; ). High-resolution computed tomography findings from adult patients with influenza A (H1N1) virus-associated pneumonia. . Eur J Radiol 74:, 93–98. [CrossRef].[PubMed]
    [Google Scholar]
  10. Mollura D. J. , Asnis D. S. , Crupi R. S. , Conetta R. , Feigin D. S. , Bray M. , Taubenberger J. K. , Bluemke D. A. . ( 2009; ). Imaging findings in a fatal case of pandemic swine-origin influenza A (H1N1). . AJR Am J Roentgenol 193:, 1500–1503. [CrossRef].[PubMed]
    [Google Scholar]
  11. Munster V. J. , de Wit E. , van den Brand J. M. , Herfst S. , Schrauwen E. J. , Bestebroer T. M. , van de Vijver D. , Boucher C. A. , Koopmans M. et al. ( 2009; ). Pathogenesis and transmission of swine-origin 2009 A(H1N1) influenza virus in ferrets. . Science 325:, 481–483.[PubMed]
    [Google Scholar]
  12. Osterhaus A. D. . ( 2008; ). New respiratory viruses of humans. . Pediatr Infect Dis J 27: Suppl. S71–S74. [CrossRef].[PubMed]
    [Google Scholar]
  13. Perez-Padilla R. , de la Rosa-Zamboni D. , Ponce de Leon S. , Hernandez M. , Quiñones-Falconi F. , Bautista E. , Ramirez-Venegas A. , Rojas-Serrano J. , Ormsby C. E. et al. ( 2009; ). Pneumonia and respiratory failure from swine-origin influenza A (H1N1) in Mexico. . N Engl J Med 361:, 680–689. [CrossRef].[PubMed]
    [Google Scholar]
  14. van den Brand J. M. , Stittelaar K. J. , van Amerongen G. , Rimmelzwaan G. F. , Simon J. , de Wit E. , Munster V. , Bestebroer T. , Fouchier R. A. et al. ( 2010; ). Severity of pneumonia due to new H1N1 influenza virus in ferrets is intermediate between that due to seasonal H1N1 virus and highly pathogenic avian influenza H5N1 virus. . J Infect Dis 201:, 993–999. [CrossRef].[PubMed]
    [Google Scholar]
  15. van den Brand J. M. , Kreijtz J. H. , Bodewes R. , Stittelaar K. J. , van Amerongen G. , Kuiken T. , Simon J. , Fouchier R. A. , Del Giudice G. et al. ( 2011; ). Efficacy of vaccination with different combinations of MF59-adjuvanted and nonadjuvanted seasonal and pandemic influenza vaccines against pandemic H1N1 (2009) influenza virus infection in ferrets. . J Virol 85:, 2851–2858. [CrossRef].[PubMed]
    [Google Scholar]
  16. van Riel D. , Munster V. J. , de Wit E. , Rimmelzwaan G. F. , Fouchier R. A. , Osterhaus A. D. , Kuiken T. . ( 2007; ). Human and avian influenza viruses target different cells in the lower respiratory tract of humans and other mammals. . Am J Pathol 171:, 1215–1223. [CrossRef].[PubMed]
    [Google Scholar]
  17. Zhou B. , Li Y. , Belser J. A. , Pearce M. B. , Schmolke M. , Subba A. X. , Shi Z. , Zaki S. R. , Blau D. M. , García-Sastre A. . ( 2010; ). NS-based live attenuated H1N1 pandemic vaccines protect mice and ferrets. . Vaccine 28:, 8015–8025. [CrossRef].[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.032805-0
Loading
/content/journal/jgv/10.1099/vir.0.032805-0
Loading

Data & Media loading...

Supplements

vol. , part 8, pp. 1854 - 1858

Graph depicting the mean virus loads of oropharyngeal swabs and nose swabs

An anaesthetized and influenza virus (pandemic H1N1)-infected ferret is monitored for pneumonic (lung) changes by means of CT scan

Scanning and necropsy schedule of the ferrets ( =8) before and after influenza virus challenge [Single PDF file](181 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error