RT Journal Article SR Electronic(1) A1 Bartolomei, Giody A1 Cevik, Recep Emrah A1 Marcello, AlessandroYR 2011 T1 Modulation of hepatitis C virus replication by iron and hepcidin in Huh7 hepatocytes JF Journal of General Virology, VO 92 IS 9 SP 2072 OP 2081 DO https://doi.org/10.1099/vir.0.032706-0 PB Microbiology Society, SN 1465-2099, AB Several clinical observations point to an intricate crosstalk between iron (Fe) metabolism and chronic hepatitis C virus (HCV) infection. In this study, we wanted to investigate the molecular control that Fe levels exert on HCV replication at the hepatocyte level. In keeping with previous observations we confirmed that supra-physiological intracellular Fe induced by haemin treatment down-modulated HCV replication from subgenomic replicons. We also found that RNAi-mediated knockdown of the key Fe modulator hepcidin increased intracellular ferritin and inhibited HCV replication. Conversely, HCV replication did not modulate ferritin content in hepatocytes. Finally, we demonstrated that hepcidin is modulated at the mRNA level by alpha interferon through STAT3. We propose that in Huh7 cells hepcidin modulation leads to an unfavourable intracellular environment for HCV replication. These data may therefore contribute to a better understanding of the complex interplay between HCV and cellular physiology during infection., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.032706-0