1887

Abstract

The polydnaviruses (PDVs) are a family of DNA viruses that are symbiotically associated with parasitoid wasps. The transcription of particular genes or gene-family members have been reported for several PDVs, but no studies have characterized the spatio-temporal patterns of expression for the entire complement of predicted genes in the encapsidated genome of any PDV isolate. The braconid wasp carries the PDV bracovirus (MdBV) and parasitizes larval stage () . The encapsidated genome consists of 15 genomic segments with 51 predicted ORFs encoding proteins ≥100 aa. A majority of these ORFs form four multimember gene families (, , and ) while the remaining ORFs consist of single copy () genes. Here we used RT-PCR and quantitative real-time PCR methods to profile the encapsidated transcriptome of MdBV in and . Our results indicate that most predicted genes are expressed in . Spatial patterns of expression in differed among genes, but temporal patterns of expression were generally similar, with transcript abundance progressively declining between 24 and 120 h. A subset of , and genes were also expressed in adult female but not male . Only one encapsidated gene () was expressed in all life stages of , albeit at much lower levels than in . However, another encapsidated gene () was expressed in adult at similar levels to those detected in .

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2011-09-01
2019-10-20
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vol. , part 9, pp. 2060–2071

Primers used to amplify predicted and single copy ( ) genes in RT-PCR and qRT-PCR assays.

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