1887

Abstract

An aborted mid-gestational male Steller sea lion fetus with an attached placenta was recovered on the floor of an open floating capture trap located off Norris Rock near Denman Island, British Columbia. Viral culture of the placenta demonstrated cytopathic effect. Although no specific signal was obtained in microarray experiments using RNA obtained from viral culture, elution and sequence analysis revealed the presence of a reovirus. Complete genome pyrosequencing led to the identification of an orthoreovirus that we have tentatively named Steller sea lion reovirus (SSRV). Phylogenetic analysis revealed similarities between SSRV and orthoreoviruses of birds, bats and other mammals that suggests potential for interspecies transmission.

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2011-11-01
2019-09-16
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vol. , part 11, pp. 2558–2565

Electron micrograph of negative-stained Steller sea lion reovirus. Phylogenetic analysis of the Lambda-2 ORFs  of the genera and . Phylogenetic analysis of the Lambda-3 Mu-2, Mu-3  ORFs of the genera and . Phylogenetic analysis of the Sigma-B and Sigma-NS ORFs of the genera and . Phylogenetic analysis of the Sigma-C, p10 and p17 ORFs of the Avian and Nelson Bay reoviruses. Protein λA: the 1303 aa λA protein (143.4 kDa, pI=6.70) is the only protein product from the L1 gene, which functions as the core shell scaffold. Protein λB: the 1259 aa RNA dependent RNA polymerase λB protein (140 kDa, pI=8.37) is encoded by the L2 segment. Protein λC: the L3 segment encodes the λC protein (142.7 kDa, pI=5.92), which extends from the inner core to the outer capsid of the avian reovirion and is comparable to the λ2 protein of MRV. . Protein μA: the 737 aa M1-encoded μA protein (83.3 kDa, pI=8.68) is a minor component of the inner capsid. . Protein σC: encoded by the 3'-proximal cistron of the S1 gene, the σC (34.5 kDa, pI=4.74) is the viral cell attachment protein. . Protein σB: encoded by the S3 segment, σB (40.8 kDa, pI=6.75) is a major component of the reovirion outer capsid.

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