RT Journal Article SR Electronic(1) A1 Roy, Soumitra A1 Clawson, David S. A1 Adam, Virginie S. A1 Medina, Angelica A1 Wilson, James M.YR 2011 T1 Construction of gene transfer vectors based on simian adenovirus 7 JF Journal of General Virology, VO 92 IS 8 SP 1749 OP 1753 DO https://doi.org/10.1099/vir.0.032300-0 PB Microbiology Society, SN 1465-2099, AB The complete nucleotide sequence of an isolate of simian adenovirus 7 (SAdV-7) was determined. The genome organization of this isolate was found to be similar to that of other primate adenoviruses with two principal notable points: severe truncation of the E1A and E1B 19K proteins and an E3 region encoding only the 12.5K homologue. The viral gene products of SAdV-7 are most closely related to simian adenovirus 1 (SAdV-1), and like SAdV-1, are related to the human adenovirus species HAdV-F, such as the enteric adenoviruses HAdV-40 and HAdV-41 and the recently defined HAdV-G (HAdV-52). Two kinds of gene transfer vectors were made: a replication-competent SAdV-7-based vector with no genomic deletion, and a standard replication-incompetent vector deleted for E1. Importantly, the E1-deleted vector could be propagated to high titre by trans-complementation in human HEK 293 cells., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.032300-0