1887

Abstract

Transmission of (OMMV) is facilitated by (Wor.) Dang. An OMMV mutant (OMMVL11) containing two changes in the coat protein (CP), asparagine to tyrosine at position 189 and alanine to threonine at position 216, has been shown not to be -transmissible owing to inefficient attachment of virions to zoospores. In this study, these amino acid changes were separately introduced into the OMMV genome through site-directed mutagenesis, and the asparagine-to-tyrosine change was shown to be largely responsible for the loss of transmission. Analysis of the structure of OMMV CP by comparative modelling approaches showed that this change is located in the interior of the virus particle and the alanine-to-threonine change is exposed on the surface. The asparagine-to-tyrosine change may indirectly affect attachment via changes in the conformation of viral CP subunits, altering the receptor binding site and thus preventing binding to the fungal zoospore.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.032284-0
2011-09-01
2024-10-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/92/9/2209.html?itemId=/content/journal/jgv/10.1099/vir.0.032284-0&mimeType=html&fmt=ahah

References

  1. Andret-Link P., Fuchs M. 2005; Transmission specificity of plant viruses by vectors. J Plant Pathol 87:153–165
    [Google Scholar]
  2. Campbell R. N. 1996; Fungal transmission of plant viruses. Annu Rev Phytopathol 34:87–108 [View Article][PubMed]
    [Google Scholar]
  3. Cardoso J. M. S., Félix M. R., Clara M. I. E., Oliveira S. 2005; The complete genome sequence of a new necrovirus isolated from Olea europaea L. Arch Virol 150:815–823 [View Article][PubMed]
    [Google Scholar]
  4. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–483 [View Article][PubMed]
    [Google Scholar]
  5. DeLano W. 2002 The PyMOL Graphics System Palo Alto, CA: DeLano Scientific;
    [Google Scholar]
  6. Félix M. R., Varanda C. M. R., Cardoso J. M. S., Clara M. I. E. 2006; Plant root uptake of Olive latent virus 1 and Olive mild mosaic virus in single and mixed infections. Proceedings of the 12th Congress of the Mediterranean Phytopathological Union, Greece516–517
    [Google Scholar]
  7. Kakani K., Sgro J.-Y., Rochon D. 2001; Identification of specific cucumber necrosis virus coat protein amino acids affecting fungus transmission and zoospore attachment. J Virol 75:5576–5583 [View Article][PubMed]
    [Google Scholar]
  8. Kakani K., Reade R., Rochon D. 2004; Evidence that vector transmission of a plant virus requires conformational change in virus particles. J Mol Biol 338:507–517 [View Article][PubMed]
    [Google Scholar]
  9. Mochizuki T., Ohnishi J., Ohki T., Kanda A., Tsuda S. 2008; Amino acid substitution in the coat protein of Melon necrotic spot virus causes loss of binding to the surface of Olpidium bornovanus zoospores. J Gen Plant Pathol 74:176–181 [View Article]
    [Google Scholar]
  10. Oda Y., Saeki K., Takahashi Y., Maeda T., Naitow H., Tsukihara T., Fukuyama K. 2000; Crystal structure of tobacco necrosis virus at 2.25 Å resolution. J Mol Biol 300:153–169 [View Article][PubMed]
    [Google Scholar]
  11. Robbins M. A., Reade R. D., Rochon D. M. 1997; A cucumber necrosis virus variant deficient in fungal transmissibility contains an altered coat protein shell domain. Virology 234:138–146 [View Article][PubMed]
    [Google Scholar]
  12. Sali A., Blundell T. L. 1993; Comparative protein modelling by satisfaction of spatial restraints. J Mol Biol 234:779–815 [View Article][PubMed]
    [Google Scholar]
  13. Temmink J. H. M., Campbell R. N., Smith P. R. 1970; Specificity and site of in vitro acquisition of tobacco necrosis virus by zoopoores of Olpidium brassicae. J Gen Virol 9:201–213 [View Article]
    [Google Scholar]
  14. Varanda C., Cardoso J. M. S., Félix M. R., Oliveira S., Clara M. I. 2010; Multiplex RT-PCR for detection and identification of three necroviruses that infect olive trees. Eur J Plant Pathol 127:161–164 [View Article]
    [Google Scholar]
  15. Varanda C. M. R., Silva M. S. M. R., Félix M. R., Clara M. I. E. 2011; Evidence of Olive mild mosaic virus transmission by Olpidium brassicae.. J Plant Pathol 130:165–172 [View Article]
    [Google Scholar]
/content/journal/jgv/10.1099/vir.0.032284-0
Loading
/content/journal/jgv/10.1099/vir.0.032284-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error