1887

Abstract

Members of the family have been identified in a number of chlamydial species infecting humans (phage CPAR39 in ), other mammals (φCPG1 in , Chp2 in and Chp3 in ) and birds (Chp1 in ). This study describes the identification and genome sequencing of Chp4, an icosahedral, 4530 bp, ssDNA phage in . Chp4 is predicted to contain eight ORFs, six of which could be assigned putative functions based on sequence similarity to characterized bacteriophage. Gene order and content were highly conserved amongst chlamydiaphage, with the highest sequence variability occurring in the IN5 and INS variable regions of the VP1 major coat protein, which has been associated with host cell recognition and binding. Phylogenetic analysis of VP1 indicated that Chp4 is a member of the , and is most closely related to phage φCPG1 and CPAR39.

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2011-07-01
2019-08-25
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vol. , part 7, pp. 1733–1737

Sequence alignment of translated VP1 major viral coat proteins. Oligonucleotide primers used for DNA sequencing of Chp4.

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