1887

Abstract

Glycoprotein J (gJ) of infectious laryngotracheitis virus (ILTV) represents a major viral antigen and is dispensable for replication in cell culture and chickens. We generated gJ deletion mutants derived from the United States Department of Agriculture standard challenge strain (USDA-ch), a GFP-expressing mutant GΔgJ, a gJ deletion mutant void of any foreign DNA insertion (BΔgJ) and a gJ rescue mutant gJR with US5 restored. GΔgJ, BΔgJ and gJR were characterized in cell culture and embryonated eggs. Entry kinetic assays showed that the gJ deletion mutants did not differ in their entry kinetics from gJR. Replication kinetics strongly indicated that gJ plays an important role during egress of the virus. Differences in the abilities of the mutants to replicate in chorioallantoic membranes of chicken embryos and to release infectious virus into the allantoic fluid supported a function of gJ during the egress of ILTV from infected cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.031443-0
2011-11-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/jgv/92/11/2586.html?itemId=/content/journal/jgv/10.1099/vir.0.031443-0&mimeType=html&fmt=ahah

References

  1. Bagust T. J., Jones R. C., Guy J. S.. ( 2000; ). Avian infectious laryngotracheitis. . Rev Sci Tech 19:, 483–492.[PubMed]
    [Google Scholar]
  2. Davison A. J., Eberle R., Ehlers B., Hayward G. S., McGeoch D. J., Minson A. C., Pellett P. E., Roizman B., Studdert M. J., Thiry E.. ( 2009; ). The order Herpesvirales. . Arch Virol 154:, 171–177. [CrossRef] [PubMed]
    [Google Scholar]
  3. Devlin J. M., Browning G. F., Gilkerson J. R.. ( 2006a; ). A glycoprotein I- and glycoprotein E-deficient mutant of infectious laryngotracheitis virus exhibits impaired cell-to-cell spread in cultured cells. . Arch Virol 151:, 1281–1289. [CrossRef] [PubMed]
    [Google Scholar]
  4. Devlin J. M., Browning G. F., Hartley C. A., Kirkpatrick N. C., Mahmoudian A., Noormohammadi A. H., Gilkerson J. R.. ( 2006b; ). Glycoprotein G is a virulence factor in infectious laryngotracheitis virus. . J Gen Virol 87:, 2839–2847. [CrossRef] [PubMed]
    [Google Scholar]
  5. Dufour-Zavala L.. ( 2008; ). Epizootiology of infectious laryngotracheitis and presentation of an industry control program. . Avian Dis 52:, 1–7. [CrossRef] [PubMed]
    [Google Scholar]
  6. Fuchs W., Ziemann K., Teifke J. P., Werner O., Mettenleiter T. C.. ( 2000; ). The non-essential UL50 gene of avian infectious laryngotracheitis virus encodes a functional dUTPase which is not a virulence factor. . J Gen Virol 81:, 627–638.[PubMed]
    [Google Scholar]
  7. Fuchs W., Wiesner D., Veits J., Teifke J. P., Mettenleiter T. C.. ( 2005; ). In vitro and in vivo relevance of infectious laryngotracheitis virus gJ proteins that are expressed from spliced and nonspliced mRNAs. . J Virol 79:, 705–716. [CrossRef] [PubMed]
    [Google Scholar]
  8. Fuchs W., Veits J., Helferich D., Granzow H., Teifke J. P., Mettenleiter T. C.. ( 2007; ). Molecular biology of avian infectious laryngotracheitis virus. . Vet Res 38:, 261–279. [CrossRef] [PubMed]
    [Google Scholar]
  9. Isomura H., Stinski M. F., Murata T., Nakayama S., Chiba S., Akatsuka Y., Kanda T., Tsurumi T.. ( 2010; ). The human cytomegalovirus UL76 gene regulates the level of expression of the UL77 gene. . PLoS ONE 5:, e11901. [CrossRef] [PubMed]
    [Google Scholar]
  10. Pavlova S. P., Veits J., Blohm U., Maresch C., Mettenleiter T. C., Fuchs W.. ( 2010; ). In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus. . J Gen Virol 91:, 847–857. [CrossRef] [PubMed]
    [Google Scholar]
  11. Thureen D. R., Keeler C. L. Jr. ( 2006; ). Psittacid herpesvirus 1 and infectious laryngotracheitis virus: comparative genome sequence analysis of two avian alphaherpesviruses. . J Virol 80:, 7863–7872. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.031443-0
Loading
/content/journal/jgv/10.1099/vir.0.031443-0
Loading

Data & Media loading...

Supplements

vol. , part 11, pp. 2586 - 2589

Oligonucleotides used for the different approaches

Map of ILTV genome

Expression of gJ in a baculovirus expression system

Indirect double-immunofluorescence of LMH cells infected with ILTV

Western blot analysis of gC and gJ expression of ILTV mutants [Single PDF file](728 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error