1887

Abstract

Circoviruses are present worldwide in birds and pigs but their occurrence in fish has not yet been reported. Recently, increased mortality was observed in barbel fry () in Hungary. This paper reports the detection of previously unknown circular viral DNA genomes in barbels by the use of a circovirus-specific wide-range nested PCR. The analysis of two complete genomes (Barbel circovirus, BaCV1 and BaCV2) indicated that they belonged into a new genetic group within the family , distinct from known circoviruses and circovirus-like genomes. Their genome size was 1957 bases and contained two major ORFs similar to the capsid and replication-associated protein genes of circoviruses. A connection between the presence of the virus and clinical manifestations of the infection could not be proved.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.031344-0
2011-08-01
2019-12-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/92/8/1817.html?itemId=/content/journal/jgv/10.1099/vir.0.031344-0&mimeType=html&fmt=ahah

References

  1. Allan G. M. , Ellis J. A. . ( 2000; ). Porcine circoviruses: a review. . J Vet Diagn Invest 12:, 3–14.[PubMed].[CrossRef]
    [Google Scholar]
  2. Allan G. , Meehan B. , Todd D. , Kennedy S. , McNeilly F. , Ellis J. , Clark E. G. , Harding J. , Espuna E. et al. ( 1998; ). Novel porcine circoviruses from pigs with wasting disease syndromes. . Vet Rec 142:, 467–468.[PubMed].[CrossRef]
    [Google Scholar]
  3. Altschul S. F. , Madden T. L. , Schäffer A. A. , Zhang J. , Zhang Z. , Miller W. , Lipman D. J. . ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef].[PubMed].
    [Google Scholar]
  4. Blinkova O. , Victoria J. , Li Y. , Keele B. F. , Sanz C. , Ndjango J. B. , Peeters M. , Travis D. , Lonsdorf E. V. et al. ( 2010; ). Novel circular DNA viruses in stool samples of wild-living chimpanzees. . J Gen Virol 91:, 74–86. [CrossRef].[PubMed].
    [Google Scholar]
  5. Cságola A. , Kecskeméti S. , Kardos G. , Kiss I. , Tuboly T. . ( 2006; ). Genetic characterization of type 2 porcine circoviruses detected in Hungarian wild boars. . Arch Virol 151:, 495–507. [CrossRef].[PubMed].
    [Google Scholar]
  6. Ellis J. , Spinato M. , Yong C. , West K. , McNeilly F. , Meehan B. , Kennedy S. , Clark E. , Krakowka S. , Allan G. . ( 2003; ). Porcine circovirus 2-associated disease in Eurasian wild boar. . J Vet Diagn Invest 15:, 364–368.[PubMed].[CrossRef]
    [Google Scholar]
  7. Farkas S. L. , Gál J. . ( 2009; ). Adenovirus and mycoplasma infection in an ornate box turtle (Terrapene ornata ornata) in Hungary. . Vet Microbiol 138:, 169–173. [CrossRef].[PubMed].
    [Google Scholar]
  8. Fauquet C. M. , Mayo M. A. , Maniloff J. , Desselberger U. , Ball L. A. . ( 2005; ). Virus Taxonomy: VIIIth Report of the International Committee on Taxonomy of Viruses. Amsterdam:: Academic Press;.
    [Google Scholar]
  9. Finn R. D. , Tate J. , Mistry J. , Coggill P. C. , Sammut S. J. , Hotz H.-R. , Ceric G. , Forslund K. , Eddy S. R. et al. ( 2008; ). The Pfam protein families database. . Nucleic Acids Res 36:, D281–D288. [CrossRef]
    [Google Scholar]
  10. Halami M. Y. , Nieper H. , Müller H. , Johne R. . ( 2008; ). Detection of a novel circovirus in mute swans (Cygnus olor) by using nested broad-spectrum PCR. . Virus Res 132:, 208–212. [CrossRef].[PubMed].
    [Google Scholar]
  11. Kappe E. C. , Halami M. Y. , Schade B. , Alex M. , Hoffmann D. , Gangl A. , Meyer K. , Dekant W. , Schwarz B. A. et al. ( 2010; ). Bone marrow depletion with haemorrhagic diathesis in calves in Germany: characterization of the disease and preliminary investigations on its aetiology. . Berl Munch Tierarztl Wochenschr 123:, 31–41 (in German).[PubMed].
    [Google Scholar]
  12. Li L. , Kapoor A. , Slikas B. , Bamidele O. S. , Wang C. , Shaukat S. , Masroor M. A. , Wilson M. L. , Ndjango J. B. et al. ( 2010; ). Multiple diverse circoviruses infect farm animals and are commonly found in human and chimpanzee feces. . J Virol 84:, 1674–1682. [CrossRef].[PubMed].
    [Google Scholar]
  13. Lőrincz M. , Cságola A. , Biksi I. , Szeredi L. , Dán Á. , Tuboly T. . ( 2010; ). Detection of porcine circovirus in rodents - short communication. . Acta Vet Hung 58:, 265–268 (in Hungarian). [CrossRef].[PubMed].
    [Google Scholar]
  14. Nayar G. P. S. , Hamel A. L. , Lin L. H. , Sachvie C. , Grudeski E. , Spearman G. . ( 1999; ). Evidence for circovirus in cattle with respiratory disease and from aborted bovine fetuses. . Can Vet J 40:, 277–278.[PubMed].
    [Google Scholar]
  15. Ng T. F. , Manire C. , Borrowman K. , Langer T. , Ehrhart L. , Breitbart M. . ( 2009; ). Discovery of a novel single-stranded DNA virus from a sea turtle fibropapilloma by using viral metagenomics. . J Virol 83:, 2500–2509. [CrossRef].[PubMed].
    [Google Scholar]
  16. Rosario K. , Duffy S. , Breitbart M. . ( 2009; a). Diverse circovirus-like genome architectures revealed by environmental metagenomics. . J Gen Virol 90:, 2418–2424. [CrossRef].[PubMed].
    [Google Scholar]
  17. Rosario K. , Nilsson C. , Lim Y. W. , Ruan Y. , Breitbart M. . ( 2009; b). Metagenomic analysis of viruses in reclaimed water. . Environ Microbiol 11:, 2806–2820. [CrossRef].[PubMed].
    [Google Scholar]
  18. Rozen S. , Skaletsky H. J. . ( 2000; ). Primer3 on the WWW for general users and for biologist programmers. . In Bioinformatics Methods and Protocols: Methods in Molecular Biology, pp. 365–386. Edited by Krawetz S. , Misener S. . . Totowa, NJ:: Humana Press;.
    [Google Scholar]
  19. Tischer I. , Rasch R. , Tochtermann G. . ( 1974; ). Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines. . Zentralbl Bakteriol [Orig A] 226:, 153–167 (in German).[PubMed].
    [Google Scholar]
  20. Tischer I. , Gelderblom H. , Vettermann W. , Koch M. A. . ( 1982; ). A very small porcine virus with circular single-stranded DNA. . Nature 295:, 64–66. [CrossRef].[PubMed].
    [Google Scholar]
  21. Todd D. . ( 2000; ). Circoviruses: immunosuppressive threats to avian species: a review. . Avian Pathol 29:, 373–394. [CrossRef].[PubMed].
    [Google Scholar]
  22. Todd D. . ( 2004; ). Avian circovirus diseases: lessons for the study of PMWS. . Vet Microbiol 98:, 169–174. [CrossRef].[PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.031344-0
Loading
/content/journal/jgv/10.1099/vir.0.031344-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error