1887

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Volume 92, Issue 8

Three-dimensional reconstruction of RNA virus by electron cryo-microscopy Free

Abstract

RNA virus is a non-enveloped icosahedral ssRNA virus infectious to the harmful bloom-forming dinoflagellate, , and which is assumed to be the major natural agent controlling the host population. The viral capsid is constructed from a single gene product. Electron cryo-microscopy revealed that the virus has a diameter of 34 nm and  = 3 symmetry. The 180 quasi-equivalent monomers have an unusual arrangement in that each monomer contributes to a ‘bump’ on the surface of the protein. Though the capsid protein probably has the classic ‘jelly roll’ β-sandwich fold, this is a new packing arrangement and is distantly related to the other positive-sense ssRNA virus capsid proteins. The handedness of the structure has been determined by a novel method involving high resolution scanning electron microscopy of the negatively stained viruses and secondary electron detection.

  • Received:
  • Accepted:
  • Published Online:
© 2011 SGM
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2011-08-01
2023-05-28
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Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by electron cryo-microscopy
J. Gen. Virol. 92, 1960 (2011); https://doi.org/10.1099/vir.0.031211-0
/content/journal/jgv/10.1099/vir.0.031211-0
/content/journal/jgv/10.1099/vir.0.031211-0
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