RT Journal Article SR Electronic(1) A1 Günther, Patrick S. A1 Mikeler, Elfriede A1 Hamprecht, Klaus A1 Schneider-Schaulies, Jürgen A1 Jahn, Gerhard A1 Dennehy, Kevin M.YR 2011 T1 CD209/DC-SIGN mediates efficient infection of monocyte-derived dendritic cells by clinical adenovirus 2C isolates in the presence of bovine lactoferrin JF Journal of General Virology, VO 92 IS 8 SP 1754 OP 1759 DO https://doi.org/10.1099/vir.0.030965-0 PB Microbiology Society, SN 1465-2099, AB Adenovirus often causes respiratory infection in immunocompromised patients, but relevant attachment receptors have largely not been defined. We show that the antiviral protein bovine lactoferrin enhances infection of monocyte-derived dendritic cells (MDDC) by adenovirus species C serotype 2 (2C) isolates. Under the same conditions infection of MDDC by human cytomegalovirus was reduced. Adenoviral infection was prominently enhanced by bovine but not human lactoferrin, and was not prominently enhanced using blood monocyte-derived macrophages, suggesting that the relevant receptor is expressed on MDDC. Infection of MDDC in the presence of bovine lactoferrin was blocked by mannan, and an antibody to CD209/DC-SIGN but not isotype control or CD46 antibodies. Lastly, U937 macrophages ectopically expressing CD209/DC-SIGN, but not parental U937 cells, were efficiently infected by adenovirus 2C in the presence of bovine lactoferrin. These results may provide a tool, given the high efficiency of infection, to dissect responses by myeloid cells to clinical adenovirus isolates., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.030965-0