@article{mbs:/content/journal/jgv/10.1099/vir.0.030593-0, author = "Köhler, Anja and Gottschling, Marc and Manning, Kizzie and Lehmann, Mandy D. and Schulz, Eric and Krüger-Corcoran, Daniela and Stockfleth, Eggert and Nindl, Ingo", title = "Genomic characterization of ten novel cutaneous human papillomaviruses from keratotic lesions of immunosuppressed patients", journal= "Journal of General Virology", year = "2011", volume = "92", number = "7", pages = "1585-1594", doi = "https://doi.org/10.1099/vir.0.030593-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.030593-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Viral warts from immunosuppressed organ transplant recipients (OTR) persist over years and may progress into non-melanoma skin cancer. The types of human papillomaviruses (HPV) in such lesions are different from that seen in the general population. A subset of these lesions is not infected with the classical wart-associated HPV types. In order to gain a better understanding of the HPV types in those lesions, we isolated ten novel HPVs from persisting keratotic lesions of immunosuppressed OTRs by rolling circle amplification and subsequent long-template PCR. Additionally, we sequenced and characterized the whole genome of the ten novel HPV types. Phylogenetic analyses revealed that nine HPV types belonged to the genus Gammapapillomavirus (γ-PV) and one to the genus Betapapillomavirus. In a phylogenetic analysis using L1 fragments of human and non-human PV types, primate papillomaviruses and our novel HPV types nested within the genus γ-PV in a highly polyphyletic pattern. This study significantly broadens the knowledge concerning the diversity and evolution of the poorly known γ-PV types.", }