%0 Journal Article %A Kaneko, Hisatoshi %A Aoki, Koki %A Ishida, Susumu %A Ohno, Shigeaki %A Kitaichi, Nobuyoshi %A Ishiko, Hiroaki %A Fujimoto, Tsuguto %A Ikeda, Yoshifumi %A Nakamura, Masako %A Gonzalez, Gabriel %A Koyanagi, Kanako O. %A Watanabe, Hidemi %A Suzutani, Tatsuo %T Recombination analysis of intermediate human adenovirus type 53 in Japan by complete genome sequence %D 2011 %J Journal of General Virology, %V 92 %N 6 %P 1251-1259 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.030361-0 %I Microbiology Society, %X Human adenovirus type 53 (HAdV-53) has commonly been detected in samples from epidemic keratoconjunctivitis (EKC) patients in Japan since 1996. HAdV-53 is an intermediate virus, containing hexon-chimeric, penton base and fiber structures similar to HAdV-22 and -37, HAdV-37 and HAdV-8, respectively. HAdV-53-like intermediate strains were first isolated from EKC samples in Japan in the 1980s. Here, the complete genome sequences of three such HAdV-53-like intermediate strains (870006C, 880249C and 890357C) and four HAdV-53 strains were determined, and their relationships were analysed. The seven HAdV strains were classified into three groups, 870006C/880249C, 890357C and the four HAdV-53 strains, on the basis of phylogenetic analyses of the partial and complete genome sequences. HAdV strains within the same group showed the highest nucleotide identities (99.87–100.00 %). Like HAdV-53, the hexon loop 1 and 2 regions of 870006C, 880249C and 890357C showed the highest identity with HAdV-22. However, these strains did not show a hexon-chimeric structure similar to HAdV-22 and -37, or a penton base similar to HAdV-37. The fiber genes of 870006C and 880249C were identical to that of HAdV-37, but not HAdV-8. Thus, the three intermediate HAdVs isolated in the 1980s were similar to each other but not to HAdV-53. The recombination breakpoints were inferred by the Recombination Detection Program (rdp) using whole-genome sequences of these seven HAdV and of 12 HAdV-D strains from GenBank. HAdV-53 may have evolved from intermediate HAdVs circulating in the 1980s, and from HAdV-8, -22 and -37, by recombination of sections cut at the putative breakpoints. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.030361-0